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CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects
Open accessAbstract
Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like "genome editing" are vital for modernizing crop breeding to provide game-changing solutions to some of the "must needed" traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.
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“Research is exploring the potential of multiplex-CRISPR gene editing constructs to accelerate genetic gains in underutilized crops, focusing on traits that enhance tolerance to abiotic stresses (Sharma et al., 2022).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Sharma et al., 2022
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Stable Expression of mtlD Gene Imparts Multiple Stress Tolerance in Finger Millet
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Improved plant cytosine base editors with high editing activity, purity, and specificity
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“So far, most of the success has been achieved in monocots (Li and Xia, 2020a; Hua et al., 2020; Xu et al., 2020; Ren et al., 2021), with tomato as an exception among the dicots where editing of three genes ALS2, and PDS1 was achieved at a frequency of 6.”
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Genomics-assisted breeding for pigeonpea improvement
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Genome Editing for Sustainable Agriculture in Africa
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Genome editing opens a new era of genetic improvement in polyploid crops
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Genome Editing for Global Food Security
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Highly efficient and genotype-independent barley gene editing based on anther culture
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“This utility of the technique also needs to be extrapolated to staple food crops (Ji et al., 2020; Chennakesavulu et al., 2021).”
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Modification of cereal plant architecture by genome editing to improve yields
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A short overview of CRISPR-Cas technology and its application in viral disease control
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Harnessing genetic resources and progress in plant genomics for fonio (Digitaria spp.) improvement
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Gene tagging via CRISPR-mediated homology-directed repair in cassava
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Genome editing for crop improvement: A perspective from India
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Genomics for greater efficiency in pigeonpea hybrid breeding
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Pigeonpea (Cajanus cajan L. Millsp.)
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Self-citationVirus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System
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Bambara Groundnut [Vigna subterranea (L.) Verdc. (Fabaceae)] Usage in Human Health
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Agrobacterium: A Genome-Editing Tool-Delivery System
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Climate smart crops for food and nutritional security for semi-arid zones of Zimbabwe
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Genome editing in food and agriculture: from regulations to consumer perspectives
Atimango, A. O., Wesana, J., Kalule, S. W., Verbeke, W., & Steur, H. D. (2024) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2024.103127 · Cited by 48
Emerging applications of gene editing technologies for the development of climate-resilient crops
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Current trends and prospects in quinoa research: An approach for strategic knowledge areas
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Metadata
- Authors
- Sharma, K. K., Palakolanu, S. R., Bhattacharya, J., Shankhapal, A. R., & Bhatnagar-Mathur, P.
- Affiliations
- Energy and Resources Institute (IN)
- International Crops Research Institute for the Semi-Arid Tropics (IN)
- University of Nottingham (GB)
- Corresponding author
- Sharma, Kiran K.
- Venue
- Frontiers in Genetics
- Publisher
- Frontiers Media SA
- Published
- 2022-10-06
- Volume
- 13
- Pages
- 999207-999207
- ISSN
- 1664-8021 (ISSN-L)
- PMC
- PMC9582247
- OpenAlex
- W4303192285
- Cited by
- 24
- References
- 209
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 5 · 2023: 2 · 2022: 1
- FWCI
- 0.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Genetics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Open Access Repository of ICRISAT (International Crops Research Institute for the Semi-Arid Tropics) (acceptedVersion)
- Rothamsted Repository (Rothamsted Repository) (acceptedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Consortium of International Agricultural Research Centers
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Agricultural Productivity and Crop Improvement
- Keywords
- CRISPR/Cas genome editing, rainfed agriculture, genetic gain, base editing, prime editing, multiplex genome editing, millet, Eragrostis tef, fonio, quinoa, Vigna subterranea, Cajanus cajan, cassava, stress tolerance, nutritional improvement, yield improvement, food security
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