Characterizing the role of monocytes in T cell cancer immunotherapy using a 3d microfluidic model
Open accessAbstract
In the hepatitis-B virus-related hepatocellular carcinoma tumor microenvironment (TME), monocytes reportedly impede natural T cell functions via PD-L1/PD-1 signaling. However, it remains unclear if T cell receptor (TCR)-redirected T cells are similarly inhibited. We thus developed a 3D intrahepatic TME microfluidic model to investigate the immunosuppressive potential of monocytes toward HBV-specific TCR T cells and the role of PD-L1/PD-1 signaling. Interestingly, in our 3D static microfluidic model, we observed that monocytes suppressed only retrovirally transduced (Tdx) TCR T cell cytotoxicity toward cancer cells via PD-L1/PD-1 while mRNA electroporated (EP) TCR T cell cytotoxicity was not affected by the presence of monocytes. Importantly, when co-cultured in 2D, both Tdx and EP TCR T cell cytotoxicity toward cancer cells were not suppressed by monocytes, suggesting our 3D model as a superior tool compared to standard 2D assays for predicting TCR T cell efficacy in a pre-clinical setting, that can thus be used to improve current immunotherapy strategies.
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Adding monocytes to the 3D hydrogel with tumor spheroids suppressed the cytotoxic effect of retrovirally transduced TCR T cells.
“Moreover, adding monocytes to the 3D hydrogel with tumor spheroids suppressed the cytotoxic effect of retrovirally transduced TCR T cells [110].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [110]
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PD-1 and PD-L1 Immune Checkpoint Blockade to Treat Breast Cancer
Hartkopf, A. D., Taran, F., Wallwiener, M., Walter, C. B., Krämer, B., Grischke, E., & Brucker, S. Y. (2016) · Breast Care · doi:10.1159/000453569 · Cited by 30,989 · see citing works
Adverse Renal Effects of Immune Checkpoint Inhibitors: A Narrative Review
Wanchoo, R., Karam, S., Uppal, N. N., Barta, V. S., Deray, G., Devoe, C., Launay‐Vacher, V., Jhaveri, K. D., & on behalf of Cancer and Kidney International Network Workgroup on Immune Checkpoint Inhibitors (2017) · American Journal of Nephrology · doi:10.1159/000455014 · Cited by 15,314 · see citing works
Chimeric Antigen Receptor T Cells for Sustained Remissions in Leukemia
Maude, S. L., Frey, N., Shaw, P. A., Aplenc, R., Barrett, D. M., Bunin, N. J., Chew, A., Gonzalez, V. E., Zheng, Z., Lacey, S. F., et al. (2014) · New England Journal of Medicine · doi:10.1056/nejmoa1407222 · Cited by 5,413 · see citing works
Macrophage Diversity Enhances Tumor Progression and Metastasis
Qian, B., & Pollard, J. W. (2010) · Cell · doi:10.1016/j.cell.2010.03.014 · Cited by 5,208 · see citing works
Monocyte and macrophage heterogeneity
Gordon, S., & Taylor, P. R. (2005) · Nature Reviews Immunology · doi:10.1038/nri1733 · Cited by 5,190 · see citing works
Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion
Dong, H., Strome, S. E., Salomao, D. R., Tamura, H., Hirano, F., Flies, D. B., Roche, P. C., Lu, J., Zhu, G., Tamada, K., et al. (2002) · Nature Medicine · doi:10.1038/nm730 · Cited by 5,025 · see citing works
The future of immune checkpoint therapy
Sharma, P., & Allison, J. P. (2015) · Science · doi:10.1126/science.aaa8172 · Cited by 4,878 · see citing works
Nivolumab plus Ipilimumab in Advanced Melanoma
Wolchok, J. D., Kluger, H., Callahan, M. K., Postow, M. A., Rizvi, N. A., Lesokhin, A. M., Segal, N. H., Ariyan, C. E., Gordon, R.-A., Reed, K., et al. (2013) · New England Journal of Medicine · doi:10.1056/nejmoa1302369 · Cited by 4,230 · see citing works
Chimeric Antigen Receptor–Modified T Cells for Acute Lymphoid Leukemia
Grupp, S. A., Kalos, M., Barrett, D., Aplenc, R., Porter, D. L., Rheingold, S. R., Teachey, D. T., Chew, A., Hauck, B., Wright, J. F., et al. (2013) · New England Journal of Medicine · doi:10.1056/nejmoa1215134 · Cited by 3,509 · see citing works
Safety and Tumor Responses with Lambrolizumab (Anti–PD-1) in Melanoma
Hamid, O., Robert, C., DAUD, A. I., Hodi, F. S., Hwu, W., Kefford, R. F., Wolchok, J. D., Hersey, P., Joseph, R. W., Weber, J. S., et al. (2013) · New England Journal of Medicine · doi:10.1056/nejmoa1305133 · Cited by 3,373 · see citing works
Lee, D. W., Kochenderfer, J. N., Stetler-Stevenson, M., Cui, Y. K., Delbrook, C., Feldman, S. A., Fry, T. J., Orentas, R., Sabatino, M., Shah, N. N., et al. (2015) · The Lancet · doi:10.1016/s0140-6736(14)61403-3 · Cited by 2,898 · see citing works
T cell exclusion, immune privilege, and the tumor microenvironment
Joyce, J. A., & Fearon, D. T. (2015) · Science · doi:10.1126/science.aaa6204 · Cited by 2,494 · see citing works
Adoptive cell transfer as personalized immunotherapy for human cancer
Rosenberg, S. A., & Restifo, N. P. (2015) · Science · doi:10.1126/science.aaa4967 · Cited by 2,492 · see citing works
Distinct role of macrophages in different tumor microenvironments
Lewis, C. E., & Pollard, J. W. (2006) · Cancer Research · doi:10.1158/0008-5472.can-05-4005 · Cited by 2,225 · see citing works
Brentjens, R. J., Davila, M. L., Riviere, I., Park, J., Wang, X., Cowell, L. G., Bartido, S., Stefanski, J., Taylor, C., Olszewska, M., et al. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3005930 · Cited by 2,063 · see citing works
Robbins, P. F., Morgan, R. A., Feldman, S. A., Yang, J. C.-H., Sherry, R. M., Dudley, M. E., Wunderlich, J. R., Nahvi, A. V., Helman, L. J., Mackall, C. L., et al. (2011) · Journal of Clinical Oncology · doi:10.1200/jco.2010.32.2537 · Cited by 1,635 · see citing works
Kochenderfer, J. N., Dudley, M. E., Kassim, S. H., Somerville, R. P. T., Carpenter, R. O., Stetler-Stevenson, M., Yang, J. C., Phan, G. Q., Hughes, M. S., Sherry, R. M., et al. (2015) · Journal of Clinical Oncology · doi:10.1200/jco.2014.56.2025 · Cited by 1,595 · see citing works
Rooney, C. M., Rooney, C. M., Ng, C. Y. C., et al. (1995) · The Lancet · doi:10.1016/s0140-6736(95)91150-2 · Cited by 1,199 · see citing works
Immune cell promotion of metastasis
Kitamura, T., Qian, B.-Z., & Pollard, J. W. (2015) · Nature Reviews Immunology · doi:10.1038/nri3789 · Cited by 1,158 · see citing works
The inflammatory micro-environment in tumor progression: the role of tumor-associated macrophages.
Allavena, P., Sica, A., Solinas, G., Porta, C., & Mantovani, A. (2008) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2007.07.004 · Cited by 1,044 · see citing works
Kuang, D., Zhao, Q., Peng, C., Xu, J., Zhang, J., Wu, C. Y., & Zheng, L. (2009) · The Journal of Experimental Medicine · doi:10.1084/jem.20082173 · Cited by 897 · see citing works
Engineering tumors with 3D scaffolds
Fischbach, C., Chen, R., Matsumoto, T., Schmelzle, T., Brugge, J. S., Polverini, P. J., & Mooney, D. J. (2007) · Nature Methods · doi:10.1038/nmeth1085 · Cited by 823 · see citing works
Gao, Q., Wang, X., Qiu, S., Yamato, I., Sho, M., Nakajima, Y., Zhou, J., Li, B., Shi, Y., Xiao, Y., et al. (2009) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-08-1608 · Cited by 810 · see citing works
The use of 3-d cultures for high-throughput screening: the multicellular spheroid model.
Kunz-Schughart, L. A., Freyer, J. P., Hofstaedter, F., & Ebner, R. (2004) · SLAS DISCOVERY · doi:10.1177/1087057104265040 · Cited by 797 · see citing works
Chen, D. S., Irving, B. A., & Hodi, F. S. (2012) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-12-1362 · Cited by 648 · see citing works
Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels
Shin, Y., Han, S., Jeon, J. S., Yamamoto, K., Zervantonakis, I. K., Sudo, R., Kamm, R. D., & Chung, S. (2012) · Nature Protocols · doi:10.1038/nprot.2012.051 · Cited by 617 · see citing works
Self-citationWestin, J. R., Chu, F., Zhang, M., Fayad, L., Kwak, L. W., Fowler, N. H., Romaguera, J. E., Hagemeister, F. B., Fanale, M. A., Samaniego, F., et al. (2013) · The Lancet Oncology · doi:10.1016/s1470-2045(13)70551-5 · Cited by 528 · see citing works
Kinter, A. L., Godbout, E. J., McNally, J. P., Sereti, I., Roby, G. A., O’Shea, M. A., & Fauci, A. S. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.10.6738 · Cited by 513 · see citing works
Shi, F., Shi, M., Zeng, Z., Qi, R., Liu, Z., Zhang, J., Yang, Y., Tien, P., & Wang, F. (2010) · International Journal of Cancer · doi:10.1002/ijc.25397 · Cited by 466 · see citing works
Adriani, G., Ma, D., Pavesi, A., Kamm, R. D., & Goh, E. L. K. (2016) · Lab on a Chip · doi:10.1039/c6lc00638h · Cited by 440 · see citing works
Self-citationZhou, G., Sprengers, D., Boor, P. P. C., Doukas, M., Schutz, H. M., Mancham, S., Pedroza‐González, A., Polak, W. G., de Jonge, J., Gaspersz, M. P., et al. (2017) · Gastroenterology · doi:10.1053/j.gastro.2017.06.017 · Cited by 412 · see citing works
Adoptive cellular therapy: A race to the finish line
June, C. H., Riddell, S. R., & Schumacher, T. N. (2015) · Science Translational Medicine · doi:10.1126/scitranslmed.aaa3643 · Cited by 402 · see citing works
Huang, R., Francois, A., McGray, A. R., Miliotto, A. J., & Odunsi, K. O. (2016) · OncoImmunology · doi:10.1080/2162402x.2016.1249561 · Cited by 388 · see citing works
Cellular Constituents of Immune Escape within the Tumor Microenvironment
Kerkar, S. P., & Restifo, N. P. (2012) · Cancer Research · doi:10.1158/0008-5472.can-11-4094 · Cited by 371 · see citing works
Kuang, D., Wu, Y., Chen, N., Chéng, J., Zhuang, S., & Zheng, L. (2007) · Blood · doi:10.1182/blood-2007-01-068031 · Cited by 256 · see citing works
Penny, H. L., Sieow, J. L., Adriani, G., Yeap, W. H., See, P., Luis, B. S., Lee, B. T. K., Lee, T. K., Mak, S. Y., Ho, Y. S., et al. (2016) · OncoImmunology · doi:10.1080/2162402x.2016.1191731 · Cited by 255 · see citing works
Self-citationA 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors
Pavesi, A., Tan, A. T., Koh, S., Chia, A., Colombo, M., Antonecchia, E., Miccolis, C., Ceccarello, E., Adriani, G., Raimondi, M. T., et al. (2017) · JCI Insight · doi:10.1172/jci.insight.89762 · Cited by 238 · see citing works
Self-citationMicrofluidics: A New Tool for Modeling Cancer–Immune Interactions
Boussommier-Calleja, A., Li, R., Chen, M. B., Wong, S. C., & Kamm, R. D. (2016) · Trends in Cancer · doi:10.1016/j.trecan.2015.12.003 · Cited by 224 · cited 1× · see citing works
“Prompted by our null observations in a 2D system, we moved toward a 3D microfluidic tumor model, which, among several other advantages, is a physiological improvement from the 2D in vitro system (43, 45).”
MethodSelf-citationPD-1 upregulation is associated with HBV-specific T cell dysfunction in chronic hepatitis B patients
Peng, G., Li, S., Wu, W., Tan, X., Chen, Y., & Chen, Z. (2007) · Molecular Immunology · doi:10.1016/j.molimm.2007.07.038 · Cited by 220 · see citing works
Gehring, A. J., Xue, S., Ho, Z. Z., et al. (2010) · Journal of Hepatology · doi:10.1016/j.jhep.2010.10.025 · Cited by 217 · see citing works
Qasim, W., Brunetto, M. R., Gehring, A. J., et al. (2014) · Journal of Hepatology · doi:10.1016/j.jhep.2014.10.001 · Cited by 207 · see citing works
Antonios, J. P., Soto, H., Everson, R. G., Moughon, D., Orpilla, J. R., Shin, N. P., Sedighim, S., Treger, J., Odesa, S., Tucker, A., et al. (2017) · Neuro-Oncology · doi:10.1093/neuonc/now287 · Cited by 206 · see citing works
Markwick, L. J., Riva, A., Ryan, J. M., Cooksley, H., Palma, E., Tranah, T. H., Vijay, G. K. M., Vergis, N., Thursz, M. R., Evans, A., et al. (2014) · Gastroenterology · doi:10.1053/j.gastro.2014.11.041 · Cited by 189 · see citing works
Biochemical Signaling of PD-1 on T Cells and Its Functional Implications
Boussiotis, V. A., Chatterjee, P., & Li, L. (2014) · The Cancer Journal · doi:10.1097/ppo.0000000000000059 · Cited by 186 · see citing works
Screening therapeutic EMT blocking agents in a three-dimensional microenvironment
Aref, A. R., Huang, R. Y., Yu, W., Chua, K. N., Sun, W., Tu, T., Bai, J., Sim, W.-J., Zervantonakis, I. K., Thiery, J. P., et al. (2012) · Integrative Biology · doi:10.1039/c2ib20209c · Cited by 183 · see citing works
Self-citationPD-1 expression on human CD8 T cells depends on both state of differentiation and activation status
Sauce, D., Almeida, J. R., Larsen, M., Haro, L., Autran, B., Freeman, G. J., & Appay, V. (2007) · AIDS · doi:10.1097/qad.0b013e3282eee548 · Cited by 173 · see citing works
Extensive double humanization of both liver and hematopoiesis in FRGN mice
Wilson, E. M., Bial, J., Tarlow, B., Bial, G., Jensen, B., Greiner, D. L., Brehm, M. A., & Grompe, M. (2014) · Stem Cell Research · doi:10.1016/j.scr.2014.08.006 · Cited by 136 · see citing works
Affoué, M., Laurent, L., Ploton, D., Poupon, M., Jc, J., & Bobichon, H. (2002) · Clinical & Experimental Metastasis · doi:10.1023/a:1014594825502 · Cited by 134 · see citing works
Zhang, Y., Kang, S., Shen, J., He, J., Jiang, L., Wang, W., Guo, Z., Peng, G., Chen, G., He, J., et al. (2015) · Medicine · doi:10.1097/md.0000000000000515 · Cited by 114 · see citing works
Bai, J., Adriani, G., Dang, T.-M., Tu, T.-Y., Leong Penny, H.-X., Wong, S.-C., Kamm, R. D., & Thiery, J.-P. (2015) · Oncotarget · doi:10.18632/oncotarget.4716 · Cited by 110 · see citing works
Self-citationAdvances in microfluidics in combating infectious diseases
Tay, A. K. P., Pavesi, A., Yazdi, S. R., Lim, C. T., & Warkiani, M. E. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.02.002 · Cited by 101 · see citing works
Self-citationKoh, S., Shimasaki, N., Suwanarusk, R., et al. (2013) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2013.43 · Cited by 97 · see citing works
Tu, T., Wang, Z., Bai, J., et al. (2013) · Advanced Healthcare Materials · doi:10.1002/adhm.201300151 · Cited by 95 · see citing works
Tumor Cell Programmed Death Ligand 1-Mediated T Cell Suppression Is Overcome by Coexpression of CD80
Haile, S. T., Bosch, J. J., Agu, N., Zeender, A. M., Somasundaram, P., Srivastava, M. K., Britting, S., Wolf, J. B., Ksander, B. R., & Ostrand‐Rosenberg, S. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1003682 · Cited by 84 · see citing works
Engineering a 3D microfluidic culture platform for tumor-treating field application
Pavesi, A., Adriani, G., Tay, A. K. P., Warkiani, M. E., Yeap, W. H., Wong, S. C., & Kamm, R. D. (2016) · Scientific Reports · doi:10.1038/srep26584 · Cited by 84 · see citing works
Self-citationRodenhizer, D., Dean, T., D'Arcangelo, E., & McGuigan, A. P. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701174 · Cited by 80 · see citing works
Scheffel, M. J., Scurti, G. M., Simms, P. E., Garrett-Mayer, E. S., Mehrotra, S., Nishimura, M. I., & Voelkel‐Johnson, C. (2016) · Cancer Research · doi:10.1158/0008-5472.can-16-0587 · Cited by 75 · see citing works
Intrahepatic CD206+ macrophages contribute to inflammation in advanced viral-related liver disease
Tan-Garcia, A., Wai, L.-E., Zheng, D., Ceccarello, E., Jo, J., Banu, N., Khakpoor, A., Chia, A., Tham, C. Y. L., Tan, A. T., et al. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.04.023 · Cited by 68 · see citing works
Self-citationMicrofluidic models for adoptive cell-mediated cancer immunotherapies
Adriani, G., Pavesi, A., Tan, A. T., Bertoletti, A., Thiery, J. P., & Kamm, R. D. (2016) · Drug Discovery Today · doi:10.1016/j.drudis.2016.05.006 · Cited by 67 · see citing works
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Ho, Y. T., Adriani, G., Beyer, S., Nhan, P.-T., Kamm, R. D., & Kah, J. C. Y. (2017) · Scientific Reports · doi:10.1038/s41598-017-00750-3 · Cited by 67 · see citing works
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Moreno-Cubero, E., & Larrubia, J. R. (2016) · World Journal of Gastroenterology · doi:10.3748/wjg.v22.i28.6469 · Cited by 60 · see citing works
Lai, Y., Gelfanov, V. M., Gelfanova, V., Kulik, L. N., Chu, C., JENG, S.-W., & Liao, N. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.11.5843 · Cited by 59 · see citing works
Lai, Y. G., Gelfanov, V. M., Gelfanova, V., et al. (1999) · PubMed · Cited by 56 · see citing works
Miyazaki, Y., Fujiwara, H., Asai, H., et al. (2013) · Blood · doi:10.1182/blood-2012-11-465971 · Cited by 42 · see citing works
Görlach, A., Herter, P., Hentschel, H., Frosch, P. J., & Acker, H. (1994) · International Journal of Cancer · doi:10.1002/ijc.2910560218 · Cited by 34 · see citing works
Modeling the Blood-Brain Barrier in a 3D triple co-culture microfluidic system
Adriani, G., Ma, D., Pavesi, A., Goh, E. L. K., & Kamm, R. D. (2015) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc.2015.7318368 · Cited by 31 · see citing works
Self-citationManzo, T., Sturmheit, T., Basso, V., Petrozziello, E., Michelini, R. H., Riba, M., Freschi, M., Elia, A. R., Grioni, M., Curnis, F., et al. (2016) · Cancer Research · doi:10.1158/0008-5472.can-16-0725 · Cited by 25 · see citing works
Teng, Y. (2015) · World Journal of Gastroenterology · doi:10.3748/wjg.v22.i1.300 · Cited by 23 · see citing works
Nivolumab (anti-PD-1, BMS-936558, ONO-4538) in patients with advanced non-small cell lung cancer.
Rajan, A., & Gulley, J. L. (2014) · PubMed · doi:10.3978/j.issn.2218-6751.2014.09.02 · Cited by 20 · see citing works
Using microfluidics to investigate tumor cell extravasation and T-cell immunotherapies
Pavesi, A., Tan, A. T., Chen, M. B., Adriani, G., Bertoletti, A., & Kamm, R. D. (2015) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc.2015.7318742 · Cited by 20 · see citing works
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(2016) · Macrophage · doi:10.14800/macrophage.1222 · Cited by 14 · see citing works
Monocyte Subsets and Their Role in Tumor Progression
Doseff, A. I., & Parihar, A. (2012) · InTech eBooks · doi:10.5772/32615 · Cited by 6 · see citing works
Low dose IL-15 induces snap arming of CD44low T lymphocytes in the absence of antigen
Tamang, D., Alves, B. N., Elliott, V., et al. (2008) · Cellular Immunology · doi:10.1016/j.cellimm.2008.04.007 · Cited by 3 · see citing works
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- Authors
- Lee, S. W. L., Adriani, G., Ceccarello, E., Pavesi, A., Tan, A. T., Bertoletti, A., Kamm, R. D., & Wong, S. C.
- Affiliations
- Agency for Science, Technology and Research (SG)
- Singapore-MIT Alliance for Research and Technology (SG)
- Duke-NUS Medical School (SG)
- Corresponding author
- Kamm, Roger Dale
- Wong, Siew Cheng
- ORCID
- Venue
- Frontiers in Immunology
- Publisher
- Frontiers Media SA
- Published
- 2018-03-06
- Volume
- 9
- Issue
- MAR
- Pages
- 416-416
- ISSN
- 1664-3224 (ISSN-L)
- PMC
- PMC5845585
- OpenAlex
- W2789945528
- Cited by
- 109
- References
- 73
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 13 · 2023: 16 · 2022: 14 · 2021: 24 · 2020: 18 · 2019: 10 · 2018: 3
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Update notices
- corrigendum (2018-07-24)
- Open access
- gold
- doi.org
- Locations
- Frontiers in Immunology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- HAL (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- monocytes, hepatocellular carcinoma, hepatitis B virus, tumor microenvironment, T cell receptor T cells, TCR gene transfer, electroporation, T cell cytotoxicity, immunosuppression
- MeSH terms
- Cells, Cultured, Cytotoxicity, Immunologic, Hepatitis B, Hepatitis B virus, Carcinoma, Hepatocellular, Humans, Liver Neoplasms, Monocytes, Receptors, Antigen, T-Cell, T-Lymphocytes, Signal Transduction, Immunotherapy, Adoptive, Electroporation, Coculture Techniques, Cancer Vaccines, Microfluidics, Hep G2 Cells, Tumor Microenvironment, B7-H1 Antigen, Programmed Cell Death 1 Receptor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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