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Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models

Hedegaard, Anne, Stodolak, Szymon, James, William S., Cowley, Sally A.·2020·Frontiers in Immunology
DOI 10.3389/fimmu.2020.614972PMC PMC7753623
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Abstract

Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models.

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Figure 1 Different culture environments convey different levels of experimental control and physiological relevance, with mono-cultures at one end of the spectrum, and transplantation into intact brains at the other. The experimental question being investigated should guide the choice of culture-system.
Figure 1 Different culture environments convey different levels of experimental control and physiological relevance, with mono-cultures at one end of the spectrum, and transplantation into intact brains at the other. The experimental question being investigated should guide the choice of culture-system.CC BY · PubMed Central

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Metadata

Authors
Hedegaard, A., Stodolak, S., James, W. S., & Cowley, S. A.
Venue
Frontiers in Immunology
Publisher
Frontiers Media SA
Published
2020-12-08
Volume
11
ISSN
1664-3224
DOI
10.3389/fimmu.2020.614972
PMC
PMC7753623
Cited by
29
References
21
Licence
creativecommons.org/licenses/by/4.0/ (vor)
Funders
  • Oxford Martin School, University of Oxford
  • Biotechnology and Biological Sciences Research Council
  • Eli Lilly and Company

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