Engineering Scalable Manufacturing of High-Quality Stem Cell-Derived Cardiomyocytes for Cardiac Tissue Repair
Open accessAbstract
Recent advances in the differentiation and production of human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) have stimulated development of strategies to use these cells in human cardiac regenerative therapies. A prerequisite for clinical trials and translational implementation of hPSC-derived CMs is the ability to manufacture safe and potent cells on the scale needed to replace cells lost during heart disease. Current differentiation protocols generate fetal-like CMs that exhibit proarrhythmogenic potential. Sufficient maturation of these hPSC-derived CMs has yet to be achieved to allow these cells to be used as a regenerative medicine therapy. Insights into the native cardiac environment during heart development may enable engineering of strategies that guide hPSC-derived CMs to mature. Specifically, considerations must be made in regard to developing methods to incorporate the native intercellular interactions and biomechanical cues into hPSC-derived CM production that are conducive to scale-up.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
hPSCs enable recapitulation of important developmental steps in vitro to produce specific cardiac cell types with relative ease, high purity, and in large amounts.
“Human pluripotent stem cells (hPSCs) enable us to recapitulate important developmental steps in vitro to produce specific cardiac cell types with relative ease, high purity, and in large amounts5–7.”
From Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease (2021) · cites this paper as [5]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Adult Cardiac Stem Cells Are Multipotent and Support Myocardial Regeneration
Beltrami, A. P., Barlucchi, L., Torella, D., Baker, M., Limana, F., Chimenti, S., Kasahara, H., Rota, M., Musso, E., Urbanek, K., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00687-1 · Cited by 3,528 · see citing works
Laflamme, M. A., Chen, K. Y., Naumova, A. V., Muskheli, V., Fugate, J. A., Dupras, S. K., Reinecke, H., Xu, C., Hassanipour, M., Police, S., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1327 · Cited by 2,207 · see citing works
Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843 · cited 1× · see citing works
“In addition, hESC-derived pancreatic progenitors differentiated into mature insulin-producing β-cells that expressed prohormone convertase enzymes upon implantation below the left kidney of immune-deficient mice (46, 47).”
BackgroundKehat, I., Kenyagin‐Karsenti, D., Snir, M., Segev, H., Amit, M., Gepstein, A., Livne, E., Binah, O., Itskovitz‐Eldor, J., & Gepstein, L. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12131 · Cited by 1,814 · see citing works
Lian, X., Zhang, J., Azarin, S. M., Zhu, K., Hazeltine, L. B., Bao, X., Hsiao, C., Kamp, T. J., & Palecek, S. P. (2013) · Nature Protocols · doi:10.1038/nprot.2012.150 · Cited by 1,748 · see citing works
Self-citationLian, X., Hsiao, C., Wilson, G., Zhu, K., Hazeltine, L. B., Azarin, S. M., Raval, K. K., Zhang, J., Kamp, T. J., & Palecek, S. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1200250109 · Cited by 1,717 · see citing works
Self-citationChemically defined generation of human cardiomyocytes
Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2999 · Cited by 1,606 · see citing works
Kehat, I., Kenyagin‐Karsenti, D., Snir, M., et al. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200112131 · Cited by 1,536 · see citing works
Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts
Chong, J. J. H., Yang, X., Don, C. W., Minami, E., Liu, Y.-W., Weyers, J. J., Mahoney, W. M., Biber, B. P. V., Cook, S. M., Palpant, N. J., et al. (2014) · Nature · doi:10.1038/nature13233 · Cited by 1,404 · see citing works
Revisiting Cardiac Cellular Composition
Pinto, A. R., Ilinykh, A., Ivey, M. J., Kuwabara, J. T., D’Antoni, M. L., Debuque, R., Chandran, A., Wang, L., Arora, K., Rosenthal, N. A., et al. (2016) · Circulation Research · doi:10.1161/circresaha.115.307778 · Cited by 1,400 · see citing works
Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population
Yang, L., Soonpaa, M. H., Adler, E. D., Roepke, T. K., Kattman, S. J., Kennedy, M., Henckaerts, E., Bonham, K., Abbott, G. W., Linden, R. M., et al. (2008) · Nature · doi:10.1038/nature06894 · Cited by 1,389 · see citing works
Laflamme, M. A., & Murry, C. E. (2011) · Nature · doi:10.1038/nature10147 · Cited by 1,231 · see citing works
Kattman, S. J., Witty, A. D., Gagliardi, M., Dubois, N. C., Niapour, M., Hotta, A., Ellis, J., & Keller, G. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2010.12.008 · Cited by 1,159 · see citing works
Klug, M. G., Soonpaa, M. H., Koh, G. Y., & Field, L. J. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118769 · Cited by 1,135 · see citing works
Tohyama, S., Hattori, F., Sano, M., Hishiki, T., Nagahata, Y., Matsuura, T., Hashimoto, H., Suzuki, T., Yamashita, H., Satoh, Y., et al. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.09.013 · Cited by 1,045 · see citing works
Moretti, A., Caron, L., Nakano, A., Lam, J. T., Bernshausen, A., Chen, Y., Qyang, Y., Bu, L., Sasaki, M., Martı́n-Puig, S., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.029 · Cited by 1,037 · see citing works
Souders, C. A., Bowers, S., & Baudino, T. A. (2009) · Circulation Research · doi:10.1161/circresaha.109.209809 · Cited by 978 · see citing works
Characterization and Enrichment of Cardiomyocytes Derived From Human Embryonic Stem Cells
Xu, C., Police, S., Rao, N., & Carpenter, M. K. (2002) · Circulation Research · doi:10.1161/01.res.0000035254.80718.91 · Cited by 902 · see citing works
Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts
Shiba, Y., Gomibuchi, T., Seto, T., Wada, Y., Ichimura, H., Tanaka, Y., Ogasawara, T., Okada, K., Shiba, N., Sakamoto, K., et al. (2016) · Nature · doi:10.1038/nature19815 · Cited by 840 · see citing works
Cardiac fibroblasts, fibrosis and extracellular matrix remodeling in heart disease
Fan, D., Takawale, A., Lee, J., & Kassiri, Z. (2012) · Fibrogenesis & Tissue Repair · doi:10.1186/1755-1536-5-15 · Cited by 814 · see citing works
He, J.-Q., Ma, Y., Lee, Y., Thomson, J. A., & Kamp, T. J. (2003) · Circulation Research · doi:10.1161/01.res.0000080317.92718.99 · Cited by 800 · see citing works
Structural and Functional Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells
Lundy, S. D., Zhu, W.-Z., Regnier, M., & Laflamme, M. A. (2013) · Stem Cells and Development · doi:10.1089/scd.2012.0490 · Cited by 738 · see citing works
Cardiomyocyte proliferation contributes to heart growth in young humans
Mollova, M., Bersell, K. R., Walsh, S., Savla, J. J., Das, L. T., Park, S., Silberstein, L. E., dos Remedios, C. G., Graham, D. A., Colan, S. D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1214608110 · Cited by 728 · see citing works
Growth of engineered human myocardium with mechanical loading and vascular coculture
Tulloch, N. L., Muskheli, V., Razumova, M. V., Korte, F. S., Regnier, M., Hauch, K. D., Pabon, L., Reinecke, H., & Murry, C. E. (2011) · Circulation Research · doi:10.1161/circresaha.110.237206 · Cited by 666 · see citing works
Adult Cardiac Sca-1-positive Cells Differentiate into Beating Cardiomyocytes
Matsuura, K., Nagai, T., Nishigaki, N., Oyama, T., Nishi, J., Wada, H., Sano, M., Toko, H., Akazawa, H., Sato, T., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310822200 · Cited by 638 · see citing works
Lopaschuk, G. D., & Jaswal, J. S. (2010) · Journal of Cardiovascular Pharmacology · doi:10.1097/fjc.0b013e3181e74a14 · Cited by 636 · see citing works
Rezania, A., Bruin, J. E., Riedel, M. J., Mojibian, M., Asadi, A., Xu, J., Gauvin, R., Narayan, K., Karanu, F., O’Neil, J. J., et al. (2012) · Diabetes · doi:10.2337/db11-1711 · Cited by 582 · see citing works
Tissue engineering of vascularized cardiac muscle from human embryonic stem cells
Caspi, O., Lesman, A., Basevitch, Y., Gepstein, A., Arbel, G., Habib, I. H. M., Gepstein, L., & Levenberg, S. (2007) · Circulation Research · doi:10.1161/01.res.0000257776.05673.ff · Cited by 558 · see citing works
Dubois, N. C., Craft, A. M., Sharma, P., Elliott, D. A., Stanley, E. G., Elefanty, A. G., Gramolini, A. O., & Keller, G. M. (2011) · Nature Biotechnology · doi:10.1038/nbt.2005 · Cited by 545 · see citing works
Interspecies Chimerism with Mammalian Pluripotent Stem Cells
Wu, J., Platero-Luengo, A., Sakurai, M., Sugawara, A., Gil, M. A., Yamauchi, T., Suzuki, K., Bogliotti, Y. S., Cuello, C., Valencia, M. M., et al. (2017) · Cell · doi:10.1016/j.cell.2016.12.036 · Cited by 500 · see citing works
Ribeiro, A. J. S., Ang, Y.-S., Fu, J., Rivas, R. N., Mohamed, T., Higgs, G., Srivastava, D., & Pruitt, B. L. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1508073112 · Cited by 479 · see citing works
Narazaki, G., Uosaki, H., Teranishi, M., Okita, K., Kim, B., Matsuoka, S., Yamanaka, S., & Yamashita, J. K. (2008) · Circulation · doi:10.1161/circulationaha.108.769562 · Cited by 475 · see citing works
Yang, X., Rodriguez, M. L., Pabon, L., Fischer, K. A., Reinecke, H., Regnier, M., Sniadecki, N. J., Ruohola‐Baker, H., & Murry, C. E. (2014) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2014.04.005 · Cited by 430 · see citing works
Stevens, K. R., Kreutziger, K. L., Dupras, S. K., Korte, F. S., Regnier, M., Muskheli, V., Nourse, M. B., Bendixen, K., Reinecke, H., & Murry, C. E. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908381106 · Cited by 423 · see citing works
Shadrin, I. Y., Allen, B. W., Qian, Y., Jackman, C. P., Carlson, A. L., Juhas, M. E., & Bursac, N. (2017) · Nature Communications · doi:10.1038/s41467-017-01946-x · Cited by 420 · see citing works
Nongenetic method for purifying stem cell–derived cardiomyocytes
Hattori, F., Chen, H., Yamashita, H., Tohyama, S., Satoh, Y.-S., Yuasa, S., Li, W., Yamakawa, H., Tanaka, T., Onitsuka, T., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1403 · Cited by 418 · see citing works
Lavine, K. J., Yu, K., White, A. C., Zhang, X.-Q., Smith, C. S., Partanen, J., & Ornitz, D. (2005) · Developmental Cell · doi:10.1016/j.devcel.2004.12.002 · Cited by 414 · see citing works
Lee, J. H., Protze, S. I., Laksman, Z., Backx, P. H., & Keller, G. M. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.07.003 · Cited by 404 · see citing works
Drouin, E., Charpentier, F., Gauthier, C., Laurent, K., & Marec, H. L. (1995) · Journal of the American College of Cardiology · doi:10.1016/0735-1097(95)00167-x · Cited by 388 · see citing works
Sekine, H., Shimizu, T., Hobo, K., Sekiya, S., Yang, J., Yamato, M., Kurosawa, H., Kobayashi, E., & Okano, T. (2008) · Circulation · doi:10.1161/circulationaha.107.757286 · Cited by 373 · see citing works
Quantitative electron microscopic description of heart muscle cells
Page, E., & McCallister, L. P. (1973) · The American Journal of Cardiology · doi:10.1016/0002-9149(73)91030-8 · Cited by 370 · see citing works
Parikh, S. S., Blackwell, D. J., Gómez‐Hurtado, N., Frisk, M., Wang, L., Kim, K., Dahl, C. P., Fiane, A. E., Tønnessen, T., Kryshtal, D. O., et al. (2017) · Circulation Research · doi:10.1161/circresaha.117.311920 · Cited by 370 · see citing works
Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy
Luxán, G., Casanova, J. C., Martı́nez-Poveda, B., Prados, B., D’Amato, G., MacGrogan, D., González-Rajal, Á., Dobarro, D., Torroja, C., Martínez, F., et al. (2013) · Nature Medicine · doi:10.1038/nm.3046 · Cited by 354 · see citing works
Distinct Compartments of the Proepicardial Organ Give Rise to Coronary Vascular Endothelial Cells
Katz, T. C., Singh, M. K., Degenhardt, K. R., Rivera‐Feliciano, J., Johnson, R. L., Epstein, J. A., & Tabin, C. J. (2012) · Developmental Cell · doi:10.1016/j.devcel.2012.01.012 · Cited by 352 · see citing works
Snir, M., Kehat, I., Gepstein, A., Coleman, R., Itskovitz‐Eldor, J., Livne, E., & Gepstein, L. (2003) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00020.2003 · Cited by 336 · see citing works
Cryopreservation of Human Stem Cells for Clinical Application: A Review
Hunt, C. J. (2011) · Transfusion Medicine and Hemotherapy · doi:10.1159/000326623 · Cited by 331 · see citing works
Structural remodeling of cardiac myocytes in patients with ischemic cardiomyopathy
Gerdes, A. M., Kellerman, S. E., Moore, J. A., Muffly, K. E., Clark, L. C., Reaves, P. Y., Malec, K. B., McKeown, P. P., & Schocken, D. D. (1992) · Circulation · doi:10.1161/01.cir.86.2.426 · Cited by 307 · see citing works
Uosaki, H., Fukushima, H., Takeuchi, A., Matsuoka, S., Nakatsuji, N., Yamanaka, S., & Yamashita, J. K. (2011) · PLoS ONE · doi:10.1371/journal.pone.0023657 · Cited by 299 · see citing works
Giacomelli, E., Bellin, M., Sala, L., van Meer, B. J., Tertoolen, L. G. J., Orlova, V. V., & Mummery, C. L. (2017) · Development · doi:10.1242/dev.143438 · Cited by 280 · see citing works
Identification and selection of cardiomyocytes during human embryonic stem cell differentiation
Huber, I., Itzhaki, I., Caspi, O., Arbel, G., Tzukerman, M., Gepstein, A., Habib, M., Yankelson, L., Kehat, I., & Gepstein, L. (2007) · The FASEB Journal · doi:10.1096/fj.05-5711com · Cited by 258 · see citing works
Herron, T. J., Rocha, A. M. D., Campbell, K. F., Ponce-Balbuena, D., Willis, B. C., Guerrero-Serna, G., Liu, Q., Klos, M., Musa, H., Zarzoso, M., et al. (2016) · Circulation: Arrhythmia and Electrophysiology · doi:10.1161/circep.113.003638 · Cited by 257 · see citing works
Mihic, A., Li, J., Miyagi, Y., Gagliardi, M., Li, S.-H., Zu, J., Weisel, R. D., Keller, G., & Li, R.-K. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.12.052 · Cited by 248 · see citing works
Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues
Feric, N. T., & Radisic, M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.04.019 · Cited by 247 · see citing works
An emerging consensus on cardiac regeneration
van Berlo, J. H., & Molkentin, J. D. (2014) · Nature Medicine · doi:10.1038/nm.3764 · Cited by 244 · see citing works
Choo, A. B. H., Tan, H. L., Ang, S. N., Fong, W. J., Chin, A. C. P., Lo, J. C. Y., Zheng, L., Hentze, H., Philp, R. J., Oh, S., et al. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0576 · Cited by 244 · see citing works
Correia, C., Koshkin, A., Duarte, P., Hu, D., Teixeira, A. P., Domian, I. J., Serra, M., & Alves, P. M. (2017) · Scientific Reports · doi:10.1038/s41598-017-08713-4 · Cited by 237 · see citing works
Bedada, F. B., Chan, S. S.-K., Metzger, S. K., Zhang, L., Zhang, J. J., Garry, D. J., Kamp, T. J., Kyba, M., & Metzger, J. M. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.07.012 · Cited by 229 · see citing works
Angst, B. D., Khan, L. U. R., Severs, N. J., Whitely, K., ROTHERY, S. M., Thompson, R. P., Magee, A. I., & Gourdie, R. G. (1997) · Circulation Research · doi:10.1161/01.res.80.1.88 · Cited by 224 · see citing works
Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches
Miki, K., Endo, K., Takahashi, S., Funakoshi, S., Takei, I., Katayama, S., Toyoda, T., Kotaka, M., Takaki, T., Umeda, M., et al. (2015) · Cell stem cell · doi:10.1016/j.stem.2015.04.005 · Cited by 221 · see citing works
In Vitro Electrophysiological Drug Testing Using Human Embryonic Stem Cell Derived Cardiomyocytes
Caspi, O., Itzhaki, I., Kehat, I., Gepstein, A., Arbel, G., Huber, I., Satin, J., & Gepstein, L. (2008) · Stem Cells and Development · doi:10.1089/scd.2007.0280 · Cited by 221 · see citing works
Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells
Birket, M., Ribeiro, M. C., Verkerk, A., Ward, D., Leitoguinho, A. R. C., den Hartogh, S. D., Orlova, V., Devalla, H., Schwach, V., Bellin, M., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3271 · Cited by 205 · see citing works
Electrical Stimulation Promotes Maturation of Cardiomyocytes Derived from Human Embryonic Stem Cells
Chan, Y.-C., Ting, S., Lee, Y.-K., Ng, K., Zhang, J., Chen, Z., Siu, C., Oh, S. K. W., & Tse, H. (2013) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-013-9510-z · Cited by 203 · see citing works
Naturally Engineered Maturation of Cardiomyocytes
Scuderi, G. J., & Butcher, J. T. (2017) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2017.00050 · Cited by 200 · see citing works
Funakoshi, S., Miki, K., Takaki, T., Okubo, C., Hatani, T., Chonabayashi, K., Nishikawa, M., Takei, I., Oishi, A., Narita, M., et al. (2016) · Scientific Reports · doi:10.1038/srep19111 · Cited by 197 · see citing works
Palpant, N. J., Pabon, L., Friedman, C. E., Redd, M. A., Hadland, B., Zaunbrecher, R. J., Bernstein, I. D., Zheng, Y. Y., & Murry, C. E. (2016) · Nature Protocols · doi:10.1038/nprot.2016.153 · Cited by 195 · see citing works
Generation of the epicardial lineage from human pluripotent stem cells
Witty, A. D., Mihic, A., Tam, R. Y., Fisher, S. A., Mikryukov, A., Shoichet, M. S., Li, R.-K., Kattman, S. J., & Keller, G. (2014) · Nature Biotechnology · doi:10.1038/nbt.3002 · Cited by 180 · see citing works
Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes
Eng, G. M., Lee, B. W., Protas, L., Gagliardi, M., Brown, K. R., Kass, R. S., Keller, G. M., Robinson, R. B., & Vunjak‐Novakovic, G. (2016) · Nature Communications · doi:10.1038/ncomms10312 · Cited by 178 · see citing works
Lee, P., Klos, M., Bollensdorff, C., Hou, L., Ewart, P., Kamp, T. J., Zhang, J., Bizy, A., Guerrero‐Serna, G., Kohl, P. K., et al. (2012) · Circulation Research · doi:10.1161/circresaha.111.262535 · Cited by 175 · see citing works
Chen, V. C., Ye, J., Shukla, P., Hua, G., Chen, D., Lin, Z., Liu, J., Chai, J., Gold, J., Wu, J. C., et al. (2015) · Stem Cell Research · doi:10.1016/j.scr.2015.08.002 · Cited by 174 · see citing works
Kim, C., Majdi, M., Xia, P., Wei, K. A., Talantova, M. V., Spiering, S., Nelson, B., Mercola, M. A., & Chen, H.-S. V. (2009) · Stem Cells and Development · doi:10.1089/scd.2009.0349 · Cited by 174 · see citing works
Lalit, P. A., Salick, M. R., Nelson, D. O., Squirrell, J. M., Shafer, C. M., Patel, N. G., Saeed, I., Schmuck, E. G., Markandeya, Y. S., Wong, R., et al. (2016) · Cell stem cell · doi:10.1016/j.stem.2015.12.001 · Cited by 173 · see citing works
Design of Injectable Materials to Improve Stem Cell Transplantation
Marquardt, L. M., & Heilshorn, S. C. (2016) · Current Stem Cell Reports · doi:10.1007/s40778-016-0058-0 · Cited by 170 · see citing works
Small Molecules Enable Cardiac Reprogramming of Mouse Fibroblasts with a Single Factor, Oct4
Wang, H., Cao, N., Spencer, C. I., Nie, B., Ma, T., Xu, T., Zhang, Y., Wang, X., Srivastava, D., & Ding, S. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.01.038 · Cited by 166 · see citing works
Global Expression Profile of Highly Enriched Cardiomyocytes Derived from Human Embryonic Stem Cells
Xu, X. Q., Soo, S. Y., Sun, W. Y., & Zweigerdt, R. (2009) · Stem Cells · doi:10.1002/stem.166 · Cited by 164 · see citing works
Cardiac differentiation of human pluripotent stem cells in scalable suspension culture
Kempf, H., Kropp, C., Olmer, R., Martin, U., & Zweigerdt, R. (2015) · Nature Protocols · doi:10.1038/nprot.2015.089 · Cited by 161 · see citing works
Lieu, D. K., Liu, J., Siu, C.-W., McNerney, G. P., Tse, H.-F., Abu-Khalil, A., Huser, T., & Li, R. A. (2009) · Stem Cells and Development · doi:10.1089/scd.2009.0052 · Cited by 160 · see citing works
Poplawsky, J. D., Guo, W. H., Paudel, N. R., Ng, A., More, K. L., Leonard, D. N., & Yan, Y. (2016) · Nature Communications · doi:10.1038/ncomms12831 · Cited by 160 · see citing works
Chemically defined, albumin-free human cardiomyocyte generation
Lian, X., Bao, X., Zilberter, M., Westman, M., Fisahn, A., Hsiao, C., Hazeltine, L. B., Dunn, K. K., Kamp, T. J., & Palecek, S. P. (2015) · Nature Methods · doi:10.1038/nmeth.3448 · Cited by 159 · see citing works
Self-citationHuman-induced pluripotent stem cell-derived cardiomyocytes exhibit temporal changes in phenotype
Ivashchenko, C. Y., Pipes, G. C. T., Lozinskaya, I. M., Lin, Z., Xu, X., Needle, S., Grygielko, E. T., Hu, E., Toomey, J. R., Lepore, J. J., et al. (2013) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00819.2012 · Cited by 153 · see citing works
Pedrotty, D. M., Klinger, R. Y., Kirkton, R. D., & Bursac, N. (2009) · Cardiovascular Research · doi:10.1093/cvr/cvp164 · Cited by 150 · see citing works
Fong, A. H., Romero-López, M., Heylman, C. M., Keating, M., Tran, D. D., Sobrino, A., Tran, A. Q., Pham, H. H., Fimbres, C., Gershon, P. D., et al. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0027 · Cited by 140 · see citing works
Iyer, D., Gambardella, L., Bernard, W. G., Serrano, F., Mascetti, V. L., Pedersen, R. A., Talasila, A., & Sinha, S. (2015) · Development · doi:10.1242/dev.119271 · Cited by 137 · see citing works
Ruan, J.-L., Tulloch, N. L., Saiget, M., Paige, S. L., Razumova, M. V., Regnier, M., Tung, K. W. C., Keller, G. M., Pabon, L., Reinecke, H., et al. (2015) · Stem Cells · doi:10.1002/stem.2036 · Cited by 134 · see citing works
Cardiac progenitor cells for heart repair
Le, T. Y. L., & Chong, J. (2016) · Cell Death Discovery · doi:10.1038/cddiscovery.2016.52 · Cited by 132 · see citing works
Prolonged human neural stem cell maturation supports recovery in injured rodent CNS
Lu, P., Ceto, S., Wang, Y., Graham, L., Wu, D., Kumamaru, H., Staufenberg, E., & Tuszynski, M. H. (2017) · Journal of Clinical Investigation · doi:10.1172/jci92955 · Cited by 129 · see citing works
Expandable Cardiovascular Progenitor Cells Reprogrammed from Fibroblasts
Zhang, Y., Cao, N., Huang, Y., Spencer, C. I., Fu, J., Chen, Y., Liu, K., Nie, B., Xu, T., Li, K., et al. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.02.001 · Cited by 128 · see citing works
Kempf, H., Olmer, R., Haase, A., Franke, A., Bolesani, E., Schwanke, K., Diaz, D. R., Coffee, M., Göhring, G., Dräger, G., et al. (2016) · Nature Communications · doi:10.1038/ncomms13602 · Cited by 128 · see citing works
Tohyama, S., Fujita, J., Fujita, C., Yamaguchi, M., Kanaami, S., Ohno, R., Sakamoto, K., Kodama, M., Kurokawa, J., Kanazawa, H., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.08.025 · Cited by 128 · see citing works
Misfeldt, A. M., Boyle, S. C., Tompkins, K. L., Bautch, V. L., Labosky, P. A., & Baldwin, H. S. (2009) · Developmental Biology · doi:10.1016/j.ydbio.2009.06.033 · Cited by 123 · see citing works
Ravenscroft, S., Pointon, A., Williams, A. W., Cross, M. J., & Sidaway, J. E. (2016) · Toxicological Sciences · doi:10.1093/toxsci/kfw069 · Cited by 113 · see citing works
Cardiomyocytes derived from embryonic stem cells resemble cardiomyocytes of the embryonic heart tube
Fijnvandraat, A. C. (2003) · Cardiovascular Research · doi:10.1016/s0008-6363(03)00282-7 · Cited by 110 · see citing works
Power output is linearly related to MyHC content in rat skinned myocytes and isolated working hearts
Korte, F. S., Herron, T. J., Rovetto, M. J., & McDonald, K. S. (2005) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01227.2004 · Cited by 110 · see citing works
Park, S.-W., Koh, Y. J., Jeon, J., Cho, Y.-H., Jang, M.-J., Kang, Y., Kim, M., Choi, C., Cho, Y. S., Chung, H., et al. (2010) · Blood · doi:10.1182/blood-2010-04-280719 · Cited by 108 · see citing works
Krüger, M., Sachse, C., Zimmermann, W., Thomas Eschenhagen, A., Klede, S., & Linke, W. A. (2007) · Circulation Research · doi:10.1161/circresaha.107.162719 · Cited by 108 · see citing works
Lui, K. O., Zangi, L., Silva, E. A., Bu, L., Sahara, M., Li, R. A., Mooney, D. J., & Chien, K. R. (2013) · Cell Research · doi:10.1038/cr.2013.112 · Cited by 108 · see citing works
Bao, X., Lian, X. L., Hacker, T. A., Schmuck, E. G., Qian, T., Bhute, V. J., Han, T., Shi, M., Drowley, L., Plowright, A. T., et al. (2016) · Nature Biomedical Engineering · doi:10.1038/s41551-016-0003 · Cited by 107 · see citing works
Self-citationFunctional profiling of human atrial and ventricular gene expression
Barth, A. S., Merk, S., Arnoldi, E., et al. (2005) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-005-1404-8 · Cited by 103 · see citing works
Efficient Generation and Cryopreservation of Cardiomyocytes Derived from Human Embryonic Stem Cells
Xu, C., Police, S., Hassanipour, M., Li, Y., Chen, Y., Priest, C. A., O’Sullivan, C., Laflamme, M. A., Zhu, W., Biber, B. P. V., et al. (2010) · Regenerative Medicine · doi:10.2217/rme.10.91 · Cited by 101 · see citing works
Lee, D. S., Chen, J.-H., Lundy, D. J., Liu, C.-H., Hwang, S.-M., Pabon, L., Shieh, R.-C., Chen, C.-C., Wu, S.-N., Yan, Y.-T., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.08.042 · Cited by 91 · see citing works
Ting, S., Chen, A., Reuveny, S., & Oh, S. K. W. (2014) · Stem Cell Research · doi:10.1016/j.scr.2014.06.002 · Cited by 85 · see citing works
Kadota, S., Minami, I., Morone, N., Heuser, J. E., Игоревич, А. К., & Nakatsuji, N. (2012) · European Heart Journal · doi:10.1093/eurheartj/ehs418 · Cited by 82 · see citing works
Bao, X., Lian, X. L., Dunn, K. K., Shi, M., Han, T., Qian, T., Bhute, V. J., Canfield, S. G., & Palecek, S. P. (2015) · Stem Cell Research · doi:10.1016/j.scr.2015.05.004 · Cited by 73 · see citing works
Self-citationNguyen, D. C., Hookway, T. A., Wu, Q., Jha, R., Preininger, M. K., Chen, X., Easley, C. A., Spearman, P. W., Deshpande, S. R., Maher, K. O., et al. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.06.002 · Cited by 72 · see citing works
Marchionni, M. A. (1995) · Nature · doi:10.1038/378334a0 · Cited by 66 · see citing works
Lux, M., Andrée, B., Horváth, T., Nosko, A., Manikowski, D., Hilfiker‐Kleiner, D., Haverich, A., & Hilfiker, A. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.11.006 · Cited by 66 · see citing works
Pekkanen-Mattila, M., Chapman, H., Kerkelä, E., Suuronen, R., Skottman, H., Koivisto, A., & Aalto‐Setälä, K. (2010) · Experimental Biology and Medicine · doi:10.1258/ebm.2010.009345 · Cited by 64 · see citing works
Goldring, C. E., Antoine, D. J., Bonner, F. W., Crozier, J., Denning, C. N., Fontana, R. J., Hanley, N. A., Hay, D. C., Ingelman‐Sundberg, M., Juhila, S., et al. (2016) · Hepatology · doi:10.1002/hep.28886 · Cited by 58 · see citing works
Iyer, D., Gambardella, L., Bernard, W. G., Serrano, F., Mascetti, V. L., Pedersen, R. A., Sinha, S., & Talasila, A. (2016) · Development · doi:10.1242/dev.136143 · Cited by 57 · see citing works
Tavakoli, T., Xu, X. Z. S., Derby, E. G., et al. (2009) · BMC Cell Biology · doi:10.1186/1471-2121-10-44 · Cited by 52 · see citing works
Bax, N. A. M., van Marion, M. H., Shah, B., Goumans, M., Bouten, C. V. C., & van der Schaft, D. W. J. (2012) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2012.07.003 · Cited by 46 · see citing works
Suhaeri, M., Subbiah, R., Kim, S.-H., Kim, C.-H., Oh, S. J., Kim, S.-H., & Park, K. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b14020 · Cited by 44 · see citing works
Correia, C., Koshkin, A., Carido, M., Espinha, N., Šarić, T., Lima, P. A., Serra, M., & Alves, P. M. (2016) · Stem Cells Translational Medicine · doi:10.5966/sctm.2015-0238 · Cited by 42 · see citing works
Iglesias‐García, O., Baumgartner, S., Macrí-Pellizzeri, L., Rodríguez-Madoz, J. R., Abizanda, G., Guruceaga, E., Albiasu, E., Corbacho, D., Benavides, C., Soriano‐Navarro, M., et al. (2014) · Stem Cells and Development · doi:10.1089/scd.2014.0211 · Cited by 39 · see citing works
Bhute, V. J., Bao, X., Dunn, K. K., Knutson, K. R., McCurry, E. C., Jin, G., Russell, W. L., Lewis, S., Ikeda, A., & Palecek, S. P. (2017) · Theranostics · doi:10.7150/thno.19390 · Cited by 38 · see citing works
Self-citationBao, X., Bhute, V. J., Han, T., Qian, T., Lian, X. L., & Palecek, S. P. (2017) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10062 · Cited by 37 · see citing works
Self-citationPasquier, J., Gupta, R., Rioult, D., Hoarau-Véchot, J., Courjaret, R. J., Machaca, K., Suwaidi, J. M. S. A., Stanley, E. G., Rafii, S., Elliott, D. A., et al. (2017) · The Journal of Heart and Lung Transplantation · doi:10.1016/j.healun.2017.01.001 · Cited by 30 · see citing works
Kosmidis, G., Bellin, M., Ribeiro, M. C., van Meer, B. J., Oostwaard, D. W., Passier, R. C., Tertoolen, L. G. J., Mummery, C. L., & Casini, S. (2015) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2015.10.026 · Cited by 28 · see citing works
Vuorenpää, H., Penttinen, K., Heinonen, T., Pekkanen-Mattila, M., Sarkanen, J., Ylikomi, T. J., & Aalto‐Setälä, K. (2017) · Cytotechnology · doi:10.1007/s10616-017-0088-1 · Cited by 27 · see citing works
A new era of ventricular assist device surgery: less invasive procedures.
Rojas, S. V., Avsar, M., Uribarri, A., et al. (2015) · PubMed · Cited by 25 · see citing works
Portable bioreactor for perfusion and electrical stimulation of engineered cardiac tissue
Tandon, N. M., Taubman, A., Cimetta, E., Saccenti, L., & Vunjak‐Novakovic, G. (2013) · Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) · doi:10.1109/embc.2013.6610974 · Cited by 23 · see citing works
Design of large-scale manufacturing of induced pluripotent stem cell derived cardiomyocytes
Darkins, C. L., & Mandenius, C. (2013) · Process Safety and Environmental Protection · doi:10.1016/j.cherd.2013.08.021 · Cited by 22 · see citing works
Heterocellular signalling and crosstalk in the heart in ischaemia and heart failure
Hirsch, E., Nagai, R., & Thum, T. (2014) · Cardiovascular Research · doi:10.1093/cvr/cvu073 · Cited by 19 · see citing works
Li, J., Minami, I., Yu, L., Tsuji, K., Nakajima, M., Qiao, J., Suzuki, M., Shimono, K., Nakatsuji, N., Kotera, H., et al. (2016) · Stem Cells International · doi:10.1155/2016/2634013 · Cited by 19 · see citing works
Synnergren, J., Heins, N., Brolén, G., Eriksson, G., Lindahl, A. H., Hyllner, J., Olsson, B. E., Sartipy, P., & Björquist, P. (2009) · Stem Cells and Development · doi:10.1089/scd.2009.0220 · Cited by 18 · see citing works
Finding Expandable Induced Cardiovascular Progenitor Cells
Chen, I. Y., & Wu, J. C. (2016) · Circulation Research · doi:10.1161/circresaha.116.308679 · Cited by 11 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Lewis-Israeli, Y. R., Wasserman, A. H., Gabalski, M. A., Volmert, B. D., Ming, Y., Ball, K. A., Yang, W., Zou, J., Ni, G., Pajares, N., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25329-5 · Cited by 536 · see citing works
Kuckelkorn, C., Aksoy, E., Stojanovic, N., Oulahyane, L., Ritter, M., Pfannkuche, K., & Fischer, H. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202403897 · Cited by 3 · see citing works
- Authors
- Dunn, K. K., & Palecek, S. P.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Palecek, Sean P.
- ORCID
- Palecek, Sean P.
- Venue
- Frontiers in Medicine
- Publisher
- Frontiers Media SA
- Published
- 2018-04-24
- Volume
- 5
- Pages
- 110-110
- ISSN
- 2296-858X (ISSN-L)
- PMC
- PMC5928319
- OpenAlex
- W2799794650
- Cited by
- 41
- References
- 125
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 11 · 2020: 13 · 2019: 7 · 2018: 1
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Medicine (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Institutes of Health (R01EB007534, T32 GM008505)
- National Science Foundation (1547225, 1743346, 1648035)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- human pluripotent stem cell-derived cardiomyocytes, cardiomyocyte maturation, cardiac regeneration, cardiac tissue engineering, scale-up manufacturing, biomechanical cues, cell-cell interactions, heart development, regenerative medicine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
