Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber
Abstract
For cardiac tissue engineering, much attention has been given to the artificial cardiac microenvironment in which anisotropic design of scaffold and extracellular matrix (ECM) are the major cues. Here we propose poly(l-lactide-co-caprolactone) and fibroblast-derived ECM (PLCL/FDM), a hybrid scaffold that combines aligned electrospun PLCL fibers and FDM. Fibroblasts were grown on the PLCL fibers for 5-7 days and subsequently decellularized to produce PLCL/FDM. Various analyses confirmed aligned, FDM-deposited PLCL fibers. Compared to fibronectin (FN)-coated electrospun PLCL fibers (control), H9c2 cardiomyoblast differentiation was significantly effective, and neonatal rat cardiomyocyte (CM) phenotype and maturation was improved on PLCL/FDM. Moreover, a coculture platform was created using multilayer PLCL/FDM in which two different cells make indirect or direct cell-cell contacts. Such coculture platforms demonstrate their feasibility in terms of higher cell viability, efficiency of target cell harvest (>95% in noncontact; 85% in contact mode), and molecular diffusion through the PLCL/FDM layer. Coculture of primary CMs and fibroblasts exhibited much better CM phenotype and improvement of CM maturity upon either direct or indirect interactions, compared to the conventional coculture systems (transwell insert and tissue culture plate (TCP)). Taken together, our platform should be very useful and have significant contributions in investigating some scientific or practical issues of crosstalks between multiple cell types.
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Stroma-coupled aligned electrospun fiber derived from fibroblasts provides good growth environment for cardiomyocytes, and neonatal rat cardiomyocytes grown on new platform have better maturity and cell viability.
“This novel platform can provide a good growth environment for cardiomyocytes, and neonatal rat cardiomyocytes grown on the new platform have better maturity and cell viability [173].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [173]
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- Authors
- Suhaeri, M., Subbiah, R., Kim, S.-H., Kim, C.-H., Oh, S. J., Kim, S.-H., & Park, K.
- Affiliations
- Korea University of Science and Technology (KR)
- Korea Institute of Science and Technology (KR)
- Corresponding author
- Park, Kwideok
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-12-20
- Volume
- 9
- Issue
- 1
- Pages
- 224-235
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2566033726
- Cited by
- 44
- References
- 53
- Citations by year
- 2026: 6 · 2024: 3 · 2023: 10 · 2022: 9 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 2
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (2015R1A2A2A04004469)
- Korea Institute of Science and Technology (2E26640)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- decellularization, poly(L-lactide-co-ε-caprolactone), cardiac tissue engineering, cardiomyocyte maturation, neonatal rat cardiomyocytes, cell-cell contact, hybrid scaffolds, cell-cell crosstalk
- MeSH terms
- Animals, Cells, Cultured, Fibroblasts, Polyesters, Coculture Techniques, Tissue Engineering, Myocytes, Cardiac, Rats, Tissue Scaffolds, Nanofibers
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