Insights into the microbiology of the chaotropic brines of Salar de Atacama, Chile
Open accessAbstract
Microbial life inhabiting hypersaline environments belong to a limited group of extremophile or extremotolerant taxa. Natural or artificial hypersaline environments are not limited to high concentrations of NaCl, and under such conditions, specific adaptation mechanisms are necessary to permit microbial survival and growth. Argentina, Bolivia and Chile include three large salars (salt flats) which are represent the largest lithium reserves globally, and that are commonly referred to as the Lithium Triangle zone. To date, a large amount of information has been generated regarding chemical, geological, meteorological and economical perspectives of these salars. However, there is a remarkable lack of information regarding the biology of these unique environments. Here we report the presence of two bacterial strains from one of most hypersaline lithium-dominated man-made environments (55.6%) reported to date. Both isolates were classified to the Bacillus genera, but displayed differences in 16S rRNA gene and fatty acid profiles. Our results also revealed that isolates are lithium-tolerant and that they are phylogenetically differentiated to Bacillus associated with high NaCl concentration environments, and form a new clade from the “Lithium Triangle” zone. To determine osmoadaptation strategies in these microorganisms, both isolates were characterized using morphological, metabolic and physiological attributes. We suggest that our characterization of bacterial isolates from an environment highly enriched in lithium has revealed that even at such extreme salinities, scope for microbial life exists. These conditions have previously been considered such as to limit the development of life, and extends the window of life beyond high concentrations of MgCl2, as previously reported, to LiCl. Our results will support the future understanding of resistance mechanisms to saline stress beyond NaCl, and likely have value as study models for the understanding of putative extra-terrestrial (e.g. Martian) life.
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In salt flat environments, aquifers of varying salinity are hydrodynamically interrelated, sustaining systems of lagoons, meadows and peatlands (high Andean wetlands).
“In these environments, aquifers of varying salinity are hydrodynamically interrelated, sustaining systems of lagoons, meadows and peatlands (high Andean wetlands), some of which have been recognized as national parks, reserves and Ramsar sites (Dorador et al., 2009(Dorador et al., , 2018;;Thiel et al., 2010;Cubillos et al., 2019;Marazuela et al., 2019).”
From Water or mineral resource? Legal interpretations and hydrosocial configurations of lithium mining in Chile (2023) · cites this paper as Cubillos et al., 2019
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
Edgar, R. C. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh340 · Cited by 44,662 · see citing works
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
Kumar, S., Stecher, G., & Tamura, K. (2016) · Molecular Biology and Evolution · doi:10.1093/molbev/msw054 · Cited by 43,923 · see citing works
Brock Biology of Microorganisms
Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (1996) · Cited by 5,759 · see citing works
4 Lipid and Cell-Wall Analysis in Bacterial Systematics
Komagata, K., & Suzuki, K. (1988) · Methods in microbiology · doi:10.1016/s0580-9517(08)70410-0 · Cited by 3,118 · see citing works
Resistance ofBacillusEndospores to Extreme Terrestrial and Extraterrestrial Environments
Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J., & Setlow, P. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.3.548-572.2000 · Cited by 2,041 · see citing works
Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal
Vreeland, R. H., Rosenzweig, W. D., & Powers, D. W. (2000) · Nature · doi:10.1038/35038060 · Cited by 828 · see citing works
Ash, C., Farrow, J. A. E., Wallbanks, S., & Collins, M. D. (2008) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1991.tb00608.x · Cited by 697 · see citing works
(1997) · Wiley-VCH eBooks · Cited by 639 · see citing works
Toxicity of lithium to humans and the environment—A literature review
Aral, H., & Vecchio-Sadus, A. (2008) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2008.02.026 · Cited by 546 · see citing works
Recovery of lithium from Uyuni salar brine
An, J. W., Kang, D. J., Tran, K. T., Kim, M. J., Lim, T., & Tran, T. (2012) · Hydrometallurgy · doi:10.1016/j.hydromet.2012.02.008 · Cited by 509 · cited 1× · see citing works
“These natural Li reservoirs have been widely characterized from geological, hydrological, meteorological and economical viewpoints (Garret, 2004; Vila, 2010; An et al., 2012; Nacif and Lacabana, 2015).”
BackgroundPotentially biogenic carbon preserved in a 4.1 billion-year-old zircon
Bell, E. A., Boehnke, P., Harrison, T. M., & Mao, W. L. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1517557112 · Cited by 474 · see citing works
Microbial diversity of soda lakes
Jones, B. E., Grant, W. D., Duckworth, A. W., & Owenson, G. G. (1998) · Extremophiles · doi:10.1007/s007920050060 · Cited by 460 · see citing works
Enceladus’s measured physical libration requires a global subsurface ocean
Thomas, P. C., Tajeddine, R., Tiscareno, M. S., Burns, J. A., Joseph, J., Loredo, T. J., Helfenstein, P., & Porco, C. C. (2015) · Icarus · doi:10.1016/j.icarus.2015.08.037 · Cited by 413 · see citing works
Heipieper, H. J., Diefenbach, R., & Keweloh, H. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.6.1847-1852.1992 · Cited by 404 · see citing works
Fatty acids of the genus Bacillus: an example of branched-chain preference
Kaneda, T. (1977) · Bacteriological Reviews · doi:10.1128/br.41.2.391-418.1977 · Cited by 377 · see citing works
Rasmussen, K., Palacios, D. M., Calambokidis, J., Saborío, M. T., Rosa, L. D., Secchi, E. R., Steiger, G. H., Allen, J. M., & Stone, G. S. (2007) · Biology Letters · doi:10.1098/rsbl.2007.0067 · Cited by 363 · see citing works
Cavosie, A. J., Valley, J. W., Wilde, S. A., & E.I.M.F. (2005) · Earth and Planetary Science Letters · doi:10.1016/j.epsl.2005.04.028 · Cited by 360 · see citing works
The Lithium future—Resources, recycling, and the environment
Wanger, T. C. (2011) · Conservation Letters · doi:10.1111/j.1755-263x.2011.00166.x · Cited by 345 · see citing works
Is there a common water-activity limit for the three domains of life?
Stevenson, A. W., Cray, J. A., Williams, J. P., Santos, R., Sahay, R., Neuenkirchen, N., McClure, C. D., Grant, I. R., Houghton, J. D. R., Quinn, J. P., et al. (2014) · The ISME Journal · doi:10.1038/ismej.2014.219 · Cited by 332 · see citing works
Adaptation of the Membrane Lipids of a Deep-Sea Bacterium to Changes in Hydrostatic Pressure
DeLong, E. F., & Yayanos, A. A. (1985) · Science · doi:10.1126/science.3992247 · Cited by 331 · see citing works
Limits of life in MgCl 2 ‐containing environments: chaotropicity defines the window
Hallsworth, J. E., Yakimov, M. M., Golyshin, P. N., Gillion, J. L. M., D’Auria, G., Alves, F. D. L., Cono, V. L., Genovese, M., McKew, B. A., Hayes, S. L., et al. (2007) · Environmental Microbiology · doi:10.1111/j.1462-2920.2006.01212.x · Cited by 286 · see citing works
Diversity, adaptation and activity of the bacterial flora in saline environments
Zahran, H. H. (1997) · Biology and Fertility of Soils · doi:10.1007/s003740050306 · Cited by 282 · see citing works
Unique amino acid composition of proteins in halophilic bacteria
Fukuchi, S., Yoshimune, K., Wakayama, M., Moriguchi, M., & Nishikawa, K. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00150-5 · Cited by 277 · see citing works
Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes
Yoshimura, F., & Nikaido, H. (1982) · Journal of Bacteriology · doi:10.1128/jb.152.2.636-642.1982 · Cited by 276 · see citing works
Life in high salt and solute concentrations: halophilic bacteria
Kushner, D. J. (1978) · Medical Entomology and Zoology · Cited by 261 · see citing works
Spectral index maps of the radio halos in Abell 665 and Abell 2163
Feretti, L., Orrù, E., Brunetti, G., Giovannini, G., Kassim, N. E., & Setti, G. (2004) · Astronomy and Astrophysics · doi:10.1051/0004-6361: · Cited by 243 · see citing works
The Hadean-Archaean Environment
Sleep, N. H. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a002527 · Cited by 228 · see citing works
Rodríguez‐Valera, F., Ventosa, A., Juez, G., & Imhoff, J. F. (1985) · Microbial Ecology · doi:10.1007/bf02010483 · Cited by 220 · see citing works
Halobacterium mediterranei spec, nov., a New Carbohydrate-Utilizing Extreme Halophile
Rodríguez‐Valera, F., Juez, G., & Kushner, D. J. (1983) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(83)80021-6 · Cited by 213 · see citing works
Marion, G. M., Fritsen, C. H., Eicken, H. S., & Payne, M. C. (2003) · Astrobiology · doi:10.1089/153110703322736105 · Cited by 213 · see citing works
Nemeth, J., Oesch, G., & Kuster, S. P. (2014) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dku379 · Cited by 210 · see citing works
A universal measure of chaotropicity and kosmotropicity
Cray, J. A., Russell, J. T., Timson, D. J., Singhal, R. S., & Hallsworth, J. E. (2012) · Environmental Microbiology · doi:10.1111/1462-2920.12018 · Cited by 208 · see citing works
Regulation of the desaturation of fatty acids and its role in tolerance to cold and salt stress
Sakamoto, T., & Murata, N. (2002) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(02)00306-5 · Cited by 208 · see citing works
Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms
Cray, J. A., Stevenson, A. W., Ball, P., Bankar, S. B., Eleuthério, E. C. A., Ezeji, T. C., Singhal, R. S., Thevelein, J. M., Timson, D. J., & Hallsworth, J. E. (2015) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2015.02.010 · Cited by 202 · see citing works
Kaneda, T. (1967) · Journal of Bacteriology · doi:10.1128/jb.93.3.894-903.1967 · Cited by 200 · see citing works
Filippidou, S., Wunderlin, T., Junier, T., Jeanneret, N., Dorador, C., Molina, V., Johnson, D. R., & Junier, P. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01707 · Cited by 200 · see citing works
Self-citationImhoff, J. F., Sahl, H. G., Soliman, G. S. H., & Trüper, H. G. (1979) · Geomicrobiology Journal · doi:10.1080/01490457909377733 · Cited by 198 · see citing works
Alcaraz, L. D., Moreno‐Hagelsieb, G., Eguiarte, L. E., Souza, V., Herrera‐Estrella, L., & Olmedo, G. M. (2010) · BMC Genomics · doi:10.1186/1471-2164-11-332 · Cited by 196 · see citing works
Halophilic and halotolerant microorganisms-an overview and historical perspective
Larsen, H. (1986) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1986.tb01835.x · Cited by 184 · see citing works
Turk, M., Méjanelle, L., Šentjurc, M., Grimalt, J. O., Gunde‐Cimerman, N., & Plemenitaš, A. (2003) · Extremophiles · doi:10.1007/s00792-003-0360-5 · Cited by 181 · see citing works
Living at the Frontiers of Life: Extremophiles in Chile and Their Potential for Bioremediation
Orellana, R., Macaya, C. C., Bravo, G., Dorochesi, F., Cumsille, A., Albornoz, R. G. V., Rojas, C., & Seeger, M. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02309 · Cited by 177 · see citing works
Lithium in Jack Hills zircons: Evidence for extensive weathering of Earth's earliest crust
Ushikubo, T., Kita, N. T., Cavosie, A. J., Wilde, S. A., Rudnick, R. L., & Valley, J. W. (2008) · Earth and Planetary Science Letters · doi:10.1016/j.epsl.2008.05.032 · Cited by 176 · see citing works
Limits of life in hostile environments: no barriers to biosphere function?
Williams, J. P., & Hallsworth, J. E. (2009) · Environmental Microbiology · doi:10.1111/j.1462-2920.2009.02079.x · Cited by 171 · see citing works
Chaotropic solutes cause water stress in Pseudomonas putida
Hallsworth, J. E., Heim, S., & Timmis, K. N. (2003) · Environmental Microbiology · doi:10.1111/j.1462-2920.2003.00478.x · Cited by 169 · see citing works
Europa's surface color suggests an ocean rich with sodium chloride
Hand, K. P., & Carlson, R. W. (2015) · Geophysical Research Letters · doi:10.1002/2015gl063559 · Cited by 168 · see citing works
Extremophiles: from abyssal to terrestrial ecosystems and possibly beyond
Canganella, F., & Wiegel, J. (2011) · Die Naturwissenschaften · doi:10.1007/s00114-011-0775-2 · Cited by 160 · see citing works
Role of fatty acids in Bacillus environmental adaptation
Diomandé, S. E., Nguyen‐The, C., Guinebretière, M., Broussolle, V., & Brillard, J. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00813 · Cited by 150 · see citing works
Link, L. S., Sawyer, J., Venkateswaran, K. J., & Nicholson, W. L. (2003) · Microbial Ecology · doi:10.1007/s00248-003-1029-4 · Cited by 148 · see citing works
Robert, F. (2001) · Science · doi:10.1126/science.1064051 · Cited by 147 · see citing works
Ionic Strength Is a Barrier to the Habitability of Mars
Fox‐Powell, M. G., Hallsworth, J. E., Cousins, C. R., & Cockell, C. S. (2016) · Astrobiology · doi:10.1089/ast.2015.1432 · Cited by 143 · see citing works
Lapierre, L., Undeland, P., & Cox, L. J. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)77866-7 · Cited by 134 · see citing works
Modulation of lipid phase behavior by kosmotropic and chaotropic solutes
Koynova, R. D., Brankov, J. G., & Tenchov, B. G. (1997) · European Biophysics Journal · doi:10.1007/s002490050038 · Cited by 131 · see citing works
Halobaculum gomorrense gen. nov., sp. nov., a Novel Extremely Halophilic Archaeon from the Dead Sea
Oren, A., GUREVICH, P., Gemmell, R. T., & Teske, A. (1995) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-45-4-747 · Cited by 128 · see citing works
Yakimov, M. M., Cono, V. L., Spada, G. L., Bortoluzzi, G., Messina, E., Smedile, F., Arcadi, E., Borghini, M., Ferrer, M., Schmitt‐Kopplin, P., et al. (2014) · Environmental Microbiology · doi:10.1111/1462-2920.12587 · Cited by 124 · see citing works
Plant single-cell and single-cell-type metabolomics
Misra, B. B., Assmann, S. M., & Chen, S. (2014) · Trends in Plant Science · doi:10.1016/j.tplants.2014.05.005 · Cited by 120 · see citing works
Acid saline lake systems give clues about past environments and the search for life on Mars
Benison, K. C., & Bowen, B. B. (2006) · Icarus · doi:10.1016/j.icarus.2006.02.018 · Cited by 117 · see citing works
(2004) · Springer eBooks · doi:10.1007/978-3-662-07656-9 · Cited by 114 · see citing works
Ecology and habitats of extremophiles
Kristj�nsson, J. K., & Hreggviðsson, G. Ó. (1995) · World Journal of Microbiology and Biotechnology · doi:10.1007/bf00339134 · Cited by 112 · see citing works
Dickson, J. L., Head, J. W., III, Levy, J. S., & Marchant, D. R. (2013) · Scientific Reports · doi:10.1038/srep01166 · Cited by 103 · see citing works
BACTERIA FROM PALEOZOIC SALT DEPOSITS
Dombrowski, H. (1963) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1963.tb13400.x · Cited by 101 · see citing works
Johnson, D. B. (2008) · Transactions of Nonferrous Metals Society of China · doi:10.1016/s1003-6326(09)60010-8 · Cited by 98 · see citing works
NaCl-saturated brines are thermodynamically moderate, rather than extreme, microbial habitats
Lee, C. J. D., McMullan, P. E., O’Kane, C. J., Stevenson, A. W., Santos, I. C., Roy, C., Ghosh, W., Mancinelli, R. L., Mormile, M. R., McMullan, G., et al. (2018) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuy026 · Cited by 83 · see citing works
Cavosie, A. J., Valley, J. W., & Wilde, S. A. (2007) · Developments in Precambrian Geology · doi:10.1016/s0166-2635(07)15025-8 · Cited by 82 · see citing works
Frangipani, E., Visaggio, D., Heeb, S., Kaever, V., Cámara, M., VISCA, P., & Imperi, F. (2014) · Iris (Roma Tre University) · doi:10.1111/1462-2920 · Cited by 82 · see citing works
High production of poly(3-hydroxybutyrate) from a wildBacillus megateriumBolivian strain
Rodríguez‐Contreras, A., Koller, M., Dias, M. M.-D. S., Calafell, M., Braunegg, G., & Marqués-Calvo, M. S. (2013) · Journal of Applied Microbiology · doi:10.1111/jam.12151 · Cited by 79 · see citing works
Diversity of Bacillus-like organisms isolated from deep-sea hypersaline anoxic sediments
Sass, A. M., McKew, B. A., Sass, H., Fichtel, J., Timmis, K. N., & McGenity, T. J. (2008) · Saline Systems · doi:10.1186/1746-1448-4-8 · Cited by 79 · see citing works
Removal and recovery of lithium using various microorganisms
Tsuruta, T. (2005) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.100.562 · Cited by 77 · see citing works
Ornitholimnology: Effects of grazing by the Andean flamingo ( Phoenicoparrus andinus )
Hurlbert, S. H., & Chang, C. C. Y. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.15.4766 · Cited by 77 · see citing works
Diversity of Microorganisms Isolated from Amber
Greenblatt, C. L., Davis, A., Clement, B., Kitts, C. L., Cox, T., & Cano, R. J. (1999) · Microbial Ecology · doi:10.1007/s002489900153 · Cited by 72 · see citing works
Toxicity of Lithium to Three Freshwater Organisms and the Antagonistic Effect of Sodium
Kszos, L. A., Beauchamp, J. J., & Stewart, A. J. (2003) · Ecotoxicology · doi:10.1023/a:1026160323594 · Cited by 71 · see citing works
Media and Conditions for the Growth of Halophilic and Halotolerant Bacteria and Archaea
Schneegurt, M. A. (2012) · Springer eBooks · doi:10.1007/978-94-007-5539-0_2 · Cited by 69 · see citing works
Adaptive strategies in the double-extremophilic prokaryotes inhabiting soda lakes
Banciu, H. L., & Muntyan, M. S. (2015) · Current Opinion in Microbiology · doi:10.1016/j.mib.2015.05.003 · Cited by 66 · see citing works
Tiquia-Arashiro, S. M., Davis, D. A., Hadid, H., Kasparian, S., Ismail, M., Ahly, S., Shim, J., Singh, S., & Murray, K. S. (2007) · Environmental Technology · doi:10.1080/09593332808618789 · Cited by 65 · see citing works
Deep Phylogeny--How a Tree Can Help Characterize Early Life on Earth
Gaucher, E. A., Kratzer, J. T., & Randall, R. N. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a002238 · Cited by 62 · see citing works
Microbial Diversity and Its Relationship to Planetary Protection
Crawford, R. L. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.8.4163-4168.2005 · Cited by 61 · see citing works
The immunostimulating and antimicrobial properties of lithium and antidepressants
Lieb, J. (2004) · Journal of Infection · doi:10.1016/j.jinf.2004.03.006 · Cited by 59 · see citing works
Longevity of microorganisms in natural environments.
Gest, H., & Mandelstam, J. (1987) · PubMed · Cited by 57 · see citing works
Microbial diversity of the hypersaline and lithium-rich Salar de Uyuni, Bolivia
Haferburg, G., Gröning, J. A. D., Schmidt, N., Kummer, N.-A., Erquicia, J. C., & Schlömann, M. (2017) · Microbiological Research · doi:10.1016/j.micres.2017.02.007 · Cited by 57 · see citing works
Halotolerant Bacillus diversity in hypersaline environments
Garabito, M. J., Márquez, M. C., & Ventosa, A. (1998) · Canadian Journal of Microbiology · doi:10.1139/w97-125 · Cited by 56 · see citing works
Osmoregulation in a New Haloalkaliphilic Bacillus from the Wadi Natrun (Egypt)
Weisser, J., & Trüper, H. G. (1985) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(85)80003-5 · Cited by 55 · see citing works
Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China
Lim, J.-M., Jeon, C. O., Lee, S.-M., Lee, J.-C., Xu, L., Jiang, C., & Kim, C. (2006) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.63678-0 · Cited by 54 · see citing works
Algo-bacterial communities of the Kulunda steppe (Altai Region, Russia) Soda Lakes
Samylina, O. S., Сапожников, Ф. В., Gainanova, O. Y., Ryabova, A. V., Nikitin, M. A., & Sorokin, D. Y. (2014) · Microbiology · doi:10.1134/s0026261714060162 · Cited by 54 · see citing works
Rubin, S., Marı́n, I., Gómez, M. J., Morales, E. A., Zekker, I., Martín‐Úriz, P. S., Rodríguez, N., & Amils, R. (2017) · Environmental Microbiology · doi:10.1111/1462-2920.13876 · Cited by 51 · see citing works
Cubillos, C. F., Aguilar, P., Grágeda, M., & Dorador, C. (2018) · Journal of Geophysical Research: Biogeosciences · doi:10.1029/2018jg004621 · Cited by 51 · see citing works
Self-citationGanguly, A., Chen, C., Lai, Y., Kuo, C.-C., Hsu, C., Chen, K., & Chen, L. (2009) · Journal of Materials Chemistry · doi:10.1039/b816556d · Cited by 49 · see citing works
Nicholson, W. L., & Schuerger, A. C. (2005) · Astrobiology · doi:10.1089/ast.2005.5.536 · Cited by 48 · see citing works
Halovenus aranensis gen. nov., sp. nov., an extremely halophilic archaeon from Aran-Bidgol salt lake
Makhdoumi, A., Amoozegar, M. A., & Ventosa, A. (2011) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.031419-0 · Cited by 37 · see citing works
Bacillus iranensis sp. nov., a moderate halophile from a hypersaline lake
Bagheri, M., Didari, M., Amoozegar, M. A., Schümann, P., Sánchez‐Porro, C., Mehrshad, M., & Ventosa, A. (2011) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.030874-0 · Cited by 36 · see citing works
Occurrence of viable, red-pigmented haloarchaea in the plumage of captive flamingoes
Yim, K. J., Kwon, J., Cha, I.-T., Oh, K.-S., Song, H. S., Lee, H.-W., Rhee, J., Song, E., Rho, J. R., Seo, M. L., et al. (2015) · Scientific Reports · doi:10.1038/srep16425 · Cited by 36 · see citing works
Novel Poly[(R)-3-Hydroxybutyrate]-Producing Bacterium Isolated from a Bolivian Hypersaline Lake
Marqués-Calvo, M. S., Dias, M. M. D. S., Calafell, M., Rodríguez‐Contreras, A., Braunegg, G., & Koller, M. (2013) · Hrčak Portal of scientific journals of Croatia (University Computing Centre) · Cited by 35 · see citing works
Using the Pareto Distribution to Improve Estimates of Topcoded Earnings
Armour, P., Burkhauser, R. V., & Larrimore, J. (2014) · National Bureau of Economic Research · doi:10.3386/w19846 · Cited by 35 · see citing works
Distribution pattern of soil microbial population in salt-affected soils
Ragab, M. (1993) · Tasks for vegetation science · doi:10.1007/978-94-011-1858-3_48 · Cited by 33 · see citing works
Pollen has a microbiome: implications for plant reproduction, insect pollination and human allergies
ZASLOFF, M. A. (2017) · Environmental Microbiology · doi:10.1111/1462-2920.13661 · Cited by 32 · see citing works
Navarro-González, R., Navarro, K. F., Coll, P., McKay, C. P., Stern, J. C., Sutter, B., Archer, P. D., Buch, A., Cabane, M., Conrad, P. G., et al. (2018) · Journal of Geophysical Research Planets · doi:10.1029/2018je005852 · Cited by 31 · see citing works
Geoquimica del Salar de Atacama, parte 1: origen de los componentes y balance salino
Alonso, H., & Risacher, F. (1996) · Andean geology · doi:10.5027/andgeov23n2-a01 · Cited by 30 · see citing works
IMMERSION REFRACTOMETRY OF SOME HALOPHILIC BACTERIA
Payne, J. I., SEHGAL, S. N., & Gibbons, N. E. (1960) · Canadian Journal of Microbiology · doi:10.1139/m60-002 · Cited by 29 · see citing works
Kemp, B. L., Tabish, E., Wolford, A., Jones, D., Butler, J. K., & Baxter, B. K. (2018) · Diversity · doi:10.3390/d10040124 · Cited by 28 · see citing works
Salar del Hombre Muerto, source of lithium-tolerant bacteria
Martínez, F. L., Orce, I. G., Rajal, V. B., & Irazusta, V. P. (2018) · Environmental Geochemistry and Health · doi:10.1007/s10653-018-0148-2 · Cited by 28 · see citing works
Filippidou, S., Junier, T., Wunderlin, T., Kooli, W. M., Palmieri, I., Al-Dourobi, A., Molina, V., Lienhard, R., Spangenberg, J. E., Johnson, S. L., et al. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00102 · Cited by 28 · see citing works
Self-citationBenison, K. C. (2019) · Journal of Sedimentary Research · doi:10.2110/jsr.2019.9 · Cited by 28 · see citing works
Amoozegar, M. A., Bagheri, M., Didari, M., Mehrshad, M., Schümann, P., Spröer, C., Sánchez‐Porro, C., & Ventosa, A. (2014) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.065375-0 · Cited by 27 · see citing works
Ravel, J., & Fraser, C. M. (2005) · Trends in Microbiology · doi:10.1016/j.tim.2005.01.004 · Cited by 25 · see citing works
Belfiore, C., Curia, M. V., & Farı́as, M. E. (2017) · Revista Argentina de Microbiología · doi:10.1016/j.ram.2017.07.005 · Cited by 22 · see citing works
Halophilic and halotolerant microorganisms-an overview and historical perspective
Larsen, H. (1986) · FEMS Microbiology Letters · doi:10.1016/0378-1097(86)90053-4 · Cited by 20 · see citing works
Oceanobacillus halophilus sp. nov., a novel moderately halophilic bacterium from a hypersaline lake
Amoozegar, M. A., Bagheri, M., Makhdoumi, A., Nikou, M. M., Fazeli, S. A. S., Schümann, P., Spröer, C., Sánchez‐Porro, C., & Ventosa, A. (2016) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijsem.0.000952 · Cited by 19 · see citing works
Maheshwari, D. K., & Saraf, M. S. (2015) · Sustainable development and biodiversity · doi:10.1007/978-3-319-14595-2 · Cited by 19 · see citing works
Environmental Influences on Tobacco Use Among Asian American and Pacific Islander Youth
Tanjasiri, S. P., Lew, R., Mouttapa, M., Lipton, R., Lew, L. S., Has, S., & Wong, M. (2013) · Health Promotion Practice · doi:10.1177/1524839913484762 · Cited by 17 · see citing works
Kamekura, M., & Onishi, H. (1982) · Canadian Journal of Microbiology · doi:10.1139/m82-020 · Cited by 17 · see citing works
Warburg, R., Moir, A., & SMITH, D. A. (1985) · Microbiology · doi:10.1099/00221287-131-2-221 · Cited by 16 · see citing works
Searching for biomolecules on Mars: Considerations for operation of a life marker chip instrument
Sephton, M., Sims, M., Court, R., Lương, D., & Cullen, D. (2013) · Planetary and Space Science · doi:10.1016/j.pss.2013.04.016 · Cited by 15 · see citing works
Life And Death Around Acid-Saline Lakes
Benison, K. C. (2008) · Palaios · doi:10.2110/palo.2008.s05 · Cited by 14 · see citing works
Kamekura, M., & Ōnishi, H. (1982) · Canadian Journal of Microbiology · doi:10.1139/m82-136 · Cited by 14 · see citing works
A lithium-rich stellar explosion
Hernanz, M. (2015) · Nature · doi:10.1038/518307a · Cited by 12 · see citing works
Dickson, J. L., Head, J. W., III, Levy, J. S., & Marchant, D. R. (2013) · Scientific Reports · doi:10.1038/srep01424 · Cited by 12 · see citing works
KURITA, N., & Funabashi, M. (1984) · Agricultural and Biological Chemistry · doi:10.1271/bbb1961.48.887 · Cited by 12 · see citing works
Ecogenomics of Deep-Ocean Microbial Bathytypes
Peoples, L. M., & Bartlett, D. (2017) · Springer eBooks · doi:10.1007/978-3-319-51686-8_2 · Cited by 10 · see citing works
Clarkson, P. B., & Morales, L. B. (2014) · Diálogo andino · doi:10.4067/s0719-26812014000200005 · Cited by 5 · see citing works
Effect of Lithium on Growth Process of Environmental Microorganism by Microcalorimetry and SEM
Li, H. R., Liu, W., Cheng, S. J., & Jiang, Y. (2014) · Advanced materials research · doi:10.4028/www.scientific.net/amr.955-959.445 · Cited by 5 · see citing works
Halotolerant Bacillus diversity in hypersaline environments
Garabito, M. J., Márquez, M. C., & Ventosa, A. (1998) · Canadian Journal of Microbiology · doi:10.1139/cjm-44-2-95 · Cited by 5 · see citing works
Kurita, N., & Funabashi, M. (1984) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1984.10866259 · Cited by 5 · see citing works
First insight into microbial diversity and ion concentration in the Uyuni salt flat, Bolivia
Pérez-Fernández, C. A., Iriarte, M., Hinojosa-Delgadillo, W., Veizaga-Salinas, A., Cano, R. J., Rivera-Perez, J., & Toranzos, G. A. (2016) · Caribbean Journal of Science · doi:10.18475/cjos.v49i1.a6 · Cited by 4 · see citing works
Sugahara, I., Hayashi, K., Kimura, T., & Jinno, C. (1983) · Institutional Repositories DataBase (IRDB) · Cited by 4 · see citing works
Wiegel, J., & Canganella, F. (2002) · Encyclopedia of Life Sciences · doi:10.1038/npg.els.0000392 · Cited by 4 · see citing works
Vilo, C., Salazar‐Ardiles, C., Caimanque, T. N., Dong, Q., Flores, N., Galetović, A., Araya, J. E., & Gómez‐Silva, B. (2019) · Microbiology Resource Announcements · doi:10.1128/mra.01549-18 · Cited by 3 · see citing works
Estudo da transferência de oxigênio em cultivo de bacillus megaterium
Lorenzini, G. C. (2012) · Lume (Universidade Federal do Rio Grande do Sul) · Cited by 2 · see citing works
A Flamboyance of (Flamingo) Microbes
Jude, B. A. (2018) · Limnology and Oceanography Bulletin · doi:10.1002/lob.10267 · Cited by 1 · see citing works
(2004) · Elsevier eBooks · doi:10.1533/9781855739062.469 · Cited by 1 · see citing works
Postigo, A. D. U., Fatkhullin, T. A., Jóhannesson, G., et al. (2007) · Max Planck Digital Library · doi:10.1051/0004-6361
Droxler (1983) · doi:10.1130/0091-7613(1983)11
Flagged first, then by citations
Research works printed but not linked: 7
- Cavoise A. Valley J. Wilde S. (1983). Evolutionary and geologic consequences of organic carbon fixing in the primitive anoxic ocean. Geology 11 141– 145. 10.1130/0091-7613198311<141:EAGCOO>2.0.CO;2 · DOI
- Garret D. E. (2004). Handbook of Lithium and Natural Calcium Chloride, Their Deposits, Processing, Uses and Properties. Amsterdam: Elsevier.
- Logan N. A. De Vos P. (2015). “ Bacillus,” in Bergey’s Manual of Systematics of Archaea and Bacteria, ed. Whitman W. B. (Hoboken, NJ: John Wiley & Sons).
- Maheshwari D. K. Saraf M. (2015). Halophiles: Biodiversity and Sustainable Exploitation. Berlin: Springer.
- Stackebrandt E. Liesack W. (1993). “ Nucleic acids and classification,” in Handbook of new Bacterial Systematics, eds Goodfellow M. O’Donnell A. G. (London: Academic Press).
- Valley J. W. Peck W. H. King E. M. Wilde S. A. (2002). A cool early Earth. Geology 30 351– 354. 10.1130/0091-76132002030<0351:ACEE>2.0.CO;2 · DOI
- Zeigler D. R. Perkins J. B. (2015). 27 The Genus Bacillus. New York, NY: CRC Press.
Cited by
Sorted by citations
Oren, A. (2024) · npj Biodiversity · doi:10.1038/s44185-024-00050-w · Cited by 75 · see citing works
Investigating Europa’s Habitability with the Europa Clipper
Vance, S. D., Craft, K. L., Shock, E. L., Schmidt, B. E., Lunine, J. I., Hand, K. P., McKinnon, W. B., Spiers, E. M., Chivers, C. J., Lawrence, J., et al. (2023) · Space Science Reviews · doi:10.1007/s11214-023-01025-2 · Cited by 80 · see citing works
Scientific novelty beyond the experiment
Hallsworth, J. E., Udaondo, Z., Pedrós‐Alió, C., Höfer, J., Benison, K. C., Lloyd, K. G., Cordero, R. J. B., de Campos, C. B. L., Yakimov, M. M., & Amils, R. (2023) · Microbial Biotechnology · doi:10.1111/1751-7915.14222 · Cited by 62 · see citing works
The elements of life: A biocentric tour of the periodic table
(2023) · Advances in Microbial Physiology · doi:10.1016/bs.ampbs.2022.11.001 · Cited by 71 · see citing works
Microbial unknowns at the saline limits for life
Hallsworth, J. E. (2019) · Nature Ecology & Evolution · doi:10.1038/s41559-019-1021-0 · Cited by 30 · see citing works
Flores Fernández, C., & Alba, R. (2023) · Frontiers in Water · doi:10.3389/frwa.2023.1075139 · Cited by 28 · see citing works
Klempay, B., Arandia‐Gorostidi, N., Dekas, A., Bartlett, D. H., Carr, C. E., Doran, P. T., Dutta, A., Erazo, N. G., Fisher, L. A., Glass, J. B., et al. (2021) · Environmental Microbiology · doi:10.1111/1462-2920.15440 · Cited by 26 · see citing works
Stable Brine Layers beneath Europa’s Chaos
Chivers, C. J., Buffo, J., & Schmidt, B. E. (2023) · The Planetary Science Journal · doi:10.3847/psj/acea75 · Cited by 23 · see citing works
Brine Volume Fraction as a Habitability Metric for Europa's Ice Shell
Wolfenbarger, N. S., Fox‐Powell, M. G., Buffo, J., Soderlund, K. M., & Blankenship, D. D. (2022) · Geophysical Research Letters · doi:10.1029/2022gl100586 · Cited by 20 · see citing works
Bruna, N., Galliani, E., Oyarzún, P., Bravo, D., Fuentes, F., & Perez-Donoso, J. M. (2022) · Biological Research · doi:10.1186/s40659-022-00382-6 · Cited by 19 · see citing works
Microbial diversity in polyextreme salt flats and their potential applications
Abdallah, M. B., Chamkha, M., Karray, F., & Sayadi, S. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-023-31644-9 · Cited by 13 · see citing works
Salazar‐Ardiles, C., Caimanque, T. N., Galetović, A., Vilo, C., Araya, J. E., Flores, N., & Gómez‐Silva, B. (2020) · Microorganisms · doi:10.3390/microorganisms8050668 · Cited by 8 · see citing works
Lithium Mining in the Salar de Atacama—Accounting Practices for Water Footprinting
Link, A., Marinova, S., Roche, L., Coroamă, V., Hinkers, L., Borchardt, D., & Finkbeiner, M. (2025) · Water · doi:10.3390/w17111670 · Cited by 6 · see citing works
Link, A., Marinova, S., Roche, L., Coroamă, V., Hinkers, L., Borchardt, D., & Finkbeiner, M. (2025) · Water · doi:10.3390/w17223311 · Cited by 6 · see citing works
Hoepfner, C., Guzmán, D., Vidal‐Veuthey, B., Foronda, V., Beggs, A., Cárdenas, J. P., Vargas, V. A., & Alfaro, F. D. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1624058 · Cited by 5 · see citing works
ФГБУН «ФИЦ питания, Б. И. Б. П., Galchenko, A. V., Sherstneva, A. A., & России», Ф. В. «. М. И. И. М. С. М. (2021) · Trace Elements in Medicine (Moscow) · doi:10.19112/2413-6174-2021-22-2-3-16 · Cited by 5 · see citing works
Weingarten, E. A., Zee, P. C., & Jackson, C. R. (2022) · Astrobiology · doi:10.1089/ast.2021.0132 · Cited by 4 · see citing works
Pereira, I. O., Herrera‐León, S., Campos‐Medina, F., & Córdova, C. R. (2026) · Sustainability Science · doi:10.1007/s11625-026-01835-7 · Cited by 3 · see citing works
Hernández-Rojas, M., Estévez, R. A., Romero, C., Pérez, S., & Labra, F. A. (2025) · Sustainability · doi:10.3390/su17188139 · Cited by 3 · see citing works
Microbial biogeochemistry and extreme environments
King, G. M., & Fenchel, T. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-15883-4.00001-3 · Cited by 1 · see citing works
Joseph, A. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-95717-5.00017-7 · Cited by 1 · see citing works
Demergasso, C. S., Avendaño, P., Escuti, C., Véliz, R., Chong, G., & Pedrós‐Alió, C. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.31.629924
Demergasso, C. S., Carvajal, P. Y. A., Escuti, C., Cruz, R. J. V., Chong, G., & Pedrós‐Alió, C. (2025) · Qeios · doi:10.32388/fgiaxm
Bruna, N., Galliani, E., Oyarzún, P., Bravo, D., Fuentes, F., & Pérez-Donoso, J. M. (2022) · Figshare · doi:10.6084/m9.figshare.19373362
Xu, R., Du, S., Liu, Z., Hu, M., Wu, J., & Zhu, H. (2026) · Current Microbiology · doi:10.1007/s00284-026-04824-2
- Authors
- Cubillos, C. F., Paredes, A., Yáñez, C., Palma, J., Severino, E., Vejar, D., Grágeda, M., & Dorador, C.
- Affiliations
- Universidad de Antofagasta (CL)
- Pontificia Universidad Católica de Valparaíso (CL)
- Corresponding author
- Cubillos, Carolina F.
- Dorador, Cristina
- ORCID
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2019-07-11
- Volume
- 10
- Pages
- 1611-1611
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC6637823
- OpenAlex
- W2960959213
- Cited by
- 25
- References
- 127
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 6 · 2022: 4 · 2021: 2 · 2020: 1 · 2019: 1
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica
- Topics
- Physical Sciences › Environmental Science › Ecology › Polar Research and Ecology
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- hypersaline brines, Salar de Atacama, lithium brines, extremophiles, Bacillus, lithium tolerance, osmoadaptation, lithium triangle, salt pans, 16S rRNA phylogeny, fatty acid composition, Mars analog, extraterrestrial life
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