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Cited by 17Plant Pathogens and Fungal DiseasesFungal and yeast genetics researchFungal Plant Pathogen Control

Aabrm1-mediated melanin synthesis is essential to growth and development, stress adaption, and pathogenicity in Alternaria alternata

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Li, Rong, Li, Yongcai, Xu, Wenyi, Liu, Wenjuan, Xu, Xiaobin, Bi, Yang, Prusky, Dov·2024·Frontiers in Microbiology
DOI 10.3389/fmicb.2023.1327765PMC PMC10808324
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Abstract

Scytalone dehydratase (brm1) is one of the key enzymes in 1, 8-dihydroxynaphthalene (DHN) melanin synthesis, which mediates melanin biosythesis and regulates cell biological process of plant fungi, but its function in Alternaria alternata, the causal agent of pear black spot, is unclear. Brm1 in A. alternata was cloned, identified, and named as Aabrm1. An Aabrm1-deletion mutant was generated and revealed that the deletion of Aabrm1 leads to a significant decrease in melanin production and forms orange colony smooth spores. In addition, the deletion of Aabrm1 gene impaired infection structure information and penetration. The external stress resistance of Δ Aabrm1 was significantly weakened, and, in particular, it is very sensitive to oxidative stress, and the contents of H 2 O 2 and O 2.- in Δ Aabrm1 were significantly increased. Virulence of Δ Aabrm1 was reduced in non-wound-inoculated pear leaves but not changed in wound-inoculated pear fruit. These results indicated that Aabrm1-mediated melanin synthesis plays an important role in the pathogenicity of A. alternata.

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  • Deletion of BRM1 in Alternaria alternata significantly reduces the production of alternariol toxin (ATX), suggesting a functional correlation between melanin biosynthesis and mycotoxin synthesis.

    “Deletion of BRM1 significantly reduces the production of alternariol toxin (ATX), suggesting a functional correlation between melanin biosynthesis and mycotoxin synthesis.”

    From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [63]

    The Introduction reports that deleting brm1 in A. alternata reduced production of altertoxin (ATX), and the Results section reports reduced melanin after Aabrm1 deletion. This supports the suggested link, but the source calls ATX altertoxin, not alternariol, and does not say the ATX reduction was statistically significant. The ATX finding is reported from Gao et al. (2022), not measured in this paper. (LLM, conf=0.88)

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  1. Bell A. A. Wheeler M. H. (1986). Biosynthesis and functions of fungal melanins. Annu. Rev. Phytopathol. 24, 411– 451. doi: 10.1146/annurev.py.24.090186.002211 · DOI · Paper page
  2. Butler M. Day A. W. Money N. P. (2001). Pathogenic properties of fungal melanin. Mycologia 93, 1– 8. doi: 10.1080/00275514.2001.12061273 · DOI
  3. Casadevall A. Cordero R. J. B. Bryan R. Nosanchuk J. Dadachova E. (2017). Melanin, radiation, and energy transduction in Fungi. Microbiol. Spectr. 5, 1– 6. doi: 10.1128/microbiolspec.FUNK-0037-2016, PMID: 28256187 PMC11687467 · DOI · Paper page
  4. Chen X. Zhu C. X. Na Y. T. Ren D. D. Zhang C. H. He Y. F.. (2021). Compartmentalization of melanin biosynthetic enzymes contributes to self-defense against intermediate compound scytalone in Botrytis cinerea. MBio 12, e00007– e00021. doi: 10.1128/mBio.00007-21, PMID: 33758088 PMC8092192 · DOI · Paper page
  5. Cho Y. Srivastava A. Ohm R. A. Lawrence C. B. Wang K. H. Grigoriev I. V.. (2012). Transcription factor Amr1 induces melanin biosynthesis and suppresses virulence in Alternaria brassicicola. PLoS Pathog. 8: e1002974. doi: 10.1371/journal.ppat.1002974, PMID: 23133370 PMC3486909 · DOI · Paper page
  6. Chumley F. G. Valent B. (1990). Genetic analysis of melanin-deficient, nonpathogenic mutants of Magnaporthe grisea. Mol. Plant-Microbe Interact. 3, 135– 143. doi: 10.1094/MPMI-3-135 · DOI · Paper page
  7. Cordero R. J. B. Casadevall A. (2017). Functions of fungal melanin beyond virulence. Fungal Biol. Rev. 31, 99– 112. doi: 10.1016/j.fbr.2016.12.003, PMID: 31649746 PMC6812541 · DOI
  8. Derbyshire M. C. Gohari A. M. Mehrabi R. Kilaru S. Steinberg G. Ali S.. (2019). Phosphopantetheinyl transferase (Ppt)-mediated biosynthesis of lysine, but not siderophores or DHN melanin, is required for virulence of Zymoseptoria tritici on wheat. Sci. Rep. 8: 17069. doi: 10.1038/s41598-018-35223-8, PMID: 30459352 PMC6244202 · DOI · Paper page
  9. Fetzner R. Seither K. Wenderoth M. Herr A. Fischer R. (2014). Alternaria alternata transcription factor cmrA controls melanization and spore development. Microbiology 160, 1845– 1854. doi: 10.1099/mic.0.079046-0, PMID: 24972701 · DOI
  10. Gao J. X. Jing J. Chen J. (2015). Elementary coordinated expression research on genes related to the synthesis of pathogenesis-related melanin and toxin in Cochliobolus lunatus. J. Shanghai Jiaotong Univ. 33, 53– 58. doi: 10.1094/MPMI-08-20-0235-R · DOI
  11. Gao J. Wenderoth M. Doppler M. Schuhmacher R. Marko D. Fischer R. (2022). Fungal melanin biosynthesis pathway as source for fungal toxins. MBio 13: e0021922. doi: 10.1128/mbio.00219-22 35475649 PMC9239091 · DOI
  12. Gao S. G. Zeng R. Xu L. H. Song Z. W. Gao P. Dai F. M. (2020). Genome sequence and spore germination-associated transcriptome analysis of Corynespora cassiicola from cucumber. BMC Microbiol. 20: 199. doi: 10.1186/s12866-020-01873-w, PMID: 32641051 PMC7346487 · DOI
  13. Goyet V. Billard E. Pouvreau J. B. Lechat M. M. Pelletier S. Bahut M.. (2017). Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal. J. Exp. Bot. 68, 5539– 5552. doi: 10.1093/jxb/erx359, PMID: 29069455 PMC5853424 · DOI
  14. Hao Y. B. Li H. X. Zhang S. Liu Y. Z. Cao Z. Y. Dong J. H. (2022). Identification and functional analysis of StSCD family in Setosphaeria turcica. Sci. Agric. Sin. 55, 3134– 3143. doi: 10.3864/j.issn.0578-1752.2022.16.006 (in Chinese) · DOI
  15. He P. Wang Y. Wang X. Zhang X. Tian C. (2017). The mitogen-activated protein kinase CgMK1 governs appressorium formation, melanin synthesis, and plant infection of Colletotrichum gloeosporioides. Front. Microbiol. 8: 2216. doi: 10.3389/fmicb.2017.02216, PMID: 29176970 PMC5686099 · DOI
  16. Helmut S. (2015). Oxidative stress: a concept in redox biology and medicine. Redox Biol. 4, 180– 183. doi: 10.1016/j.redox.2015.01.002, PMID: 25588755 PMC4309861 · DOI
  17. Huang Y. Li Y. C. Li D. M. Bi Y. Prusky D. B. Dong Y. P.. (2020). Phospholipase C from Alternaria alternata is induced by physiochemical cues on the pear fruit surface that dictate infection structure differentiation and pathogenicity. Front. Microbiol. 11: 1279. doi: 10.3389/fmicb.2020.01279, PMID: 32695073 PMC7339947 · DOI
  18. Jia S. Chi Z. Chen L. Liu G. Hu Z. Chi Z. (2021). Genomics molecular evolution and regulation of DHN melanin-related gene clusters are closely related to adaptation of different melanin-producing fungi. Genomics 113, 1962– 1975. doi: 10.1016/j.ygeno.2021.04.034, PMID: 33901575 · DOI
  19. Jiang Q. Q. Li Y. C. Mao R. Y. Bi Y. Liu Y. X. Zhang M.. (2023). AaCaMKs positively regulate development, infection structure differentiation and pathogenicity in Alternaria alternata, causal agent of pear black spot. Int. J. Mol. Sci. 24: 1381. doi: 10.3390/ijms24021381, PMID: 36674895 PMC9865007 · DOI
  20. Kawamura C. Moriwaki J. Kimura N. Fujita Y. Fuji S. Hirano T.. (1997). The melanin biosynthesis genes of Alternaria alternata can restore pathogenicity of the melanin-deficient mutants of Magnaporthe grisea. Mol. Plant-Microbe Interact. 10, 446– 453. doi: 10.1094/MPMI.1997.10.4.446, PMID: 9150594 · DOI
  21. Kheder A. A. Akagi Y. Akamatsu H. Yanaga K. Maekawa N. Otani H.. (2012). Functional analysis of the melanin biosynthesis genes ALM1 and BRM2-1 in the tomato pathotype of Alternaria alternata. J. Gen. Plant Pathol. 78, 30– 38. doi: 10.1007/s10327-011-0356-4 · DOI
  22. Kihara J. Moriwaki A. Tanaka N. Tanaka C. Ueno M. Arase S. (2008). Characterization of the BMR1 gene encoding a transcription factor for melanin biosynthesis genes in the phytopathogenic fungus Bipolaris oryzae. FEMS Microbiol. Lett. 281, 221– 227. doi: 10.1111/j.1574-6968.2008.01101.x, PMID: 18312572 · DOI
  23. Li R. Li Y. C. Xu W. Y. Zhang M. Jiang Q. Q. Liu Y. X.. (2022). Transcription factor AacmrA mediated melanin synthesis regulates the growth, appressorium formation, stress response and pathogenicity of pear fungal Alternaria alternata. Fungal Biol. 126, 687– 695. doi: 10.1016/j.funbio.2022.08.008, PMID: 36116900 · DOI
  24. Li R. Xu W. Y. Zong Y. Y. Wang X. J. Li Y. C. Prusky D. (2023). Melanin synthesis gene Aapks contributes to appressorium formation, stress response, cell well integrity and virulence in Alternaria alternata. Postharvest Biol. Technol. 198: 112247. doi: 10.1016/j.postharvbio.2023.112247 · DOI
  25. Li H. Zhang Z. Q. He C. Qin G. Z. Tian S. P. (2016). Comparative proteomics reveals the potential targets of BcNoxR, a putative regulatory subunit of NADPH oxidase of Botrytis cinerea. Mol. Plant Microbe Interact. 29, 990– 1003. doi: 10.1094/MPMI-11-16-0227-R, PMID: 27898285 · DOI
  26. Li J. Zhou L. Yin C. Zhang D. Klosterman S. J. Wang B. L.. (2019). The Verticillium dahliae Sho1-MAPK pathway regulates melanin biosynthesis and is required for cotton infection. Environ. Microbiol. 21, 4852– 4874. doi: 10.1111/1462-2920.14846, PMID: 31667948 PMC6916341 · DOI · Paper page
  27. Liang Y. Xiong W. Steinkellner S. Feng J. (2018). Deficiency of the melanin biosynthesis genes SCD1 and THR1 affects sclerotial development and vegetative growth, but not pathogenicity, in Sclerotinia sclerotiorum. Mol. Plant Pathol. 19, 1444– 1453. doi: 10.1111/mpp.12627, PMID: 29024255 PMC6638068 · DOI · Paper page
  28. Liu Y. X. Li Y. C. Ma L. Deng H. W. Huang Y. Jiang Q. Q.. (2021). The transmembrane protein AaSho1 is essential for appressorium formation and secondary metabolism but dispensable for vegetative growth in pear fungal Alternaria alternata. Fungal Biol. 126, 139– 148. doi: 10.1016/j.funbio.2021.11.006 35078585 · DOI
  29. Liva K. J. Schmittgen T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2_ΔΔCt method. Methods 25, 402– 408. doi: 10.1006/meth.2001.1262 11846609 · DOI · Paper page
  30. Luo X. M. Mao H. Q. Wei Y. M. Cai J. Xie C. J. Sui A. P.. (2016). The fungal-specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae. Mol. Plant Pathol. 17, 1364– 1381. doi: 10.1111/mpp.12367, PMID: 26857810 PMC6638448 · DOI
  31. Luo C. W. Zhao H. Z. Yang X. X. Qiang C. C. Cheng J. S. Xie J. T.. (2018). Functional analysis of the melanin-associated gene CmMR1 in Coniothyrium minitans. Front. Microbiol. 9: 2658. doi: 10.3389/fmicb.2018.02658, PMID: 30467498 PMC6237101 · DOI
  32. Meena M. Gupta S. K. Swapnil P. Zehra A. Dubey M. K. Upadhyay R. S. (2017). Alternaria toxins: potential virulence factors and genes related to pathogenesis. Front. Microbiol. 8: 1451. doi: 10.3389/fmicb.2017.01451, PMID: 28848500 PMC5550700 · DOI
  33. Misaghi I. J. Grogan R. G. Duniway J. M. Kimble K. A. (1977). Influence of environment and culture media on spore morphology of Alternaria alternata. Phytopathology 68, 29– 34. doi: 10.1094/PHYTO-68-29 · DOI
  34. Nosanchuk J. D. Casadevall A. (2006). Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds. Antimicrob. Agents Chemother. 50, 3519– 3528. doi: 10.1128/AAC.00545-06, PMID: 17065617 PMC1635213 · DOI
  35. Prusky D. (1996). Pathogen quiescence in postharvest diseases. Annu. Rev. Phytopathol. 34, 413– 434. doi: 10.1146/annurev.phyto.34.1.413, PMID: 15012550 · DOI
  36. Steiner U. Oerke E. C. (2017). Localized melanization of appressoria is required for pathogenicity of Venturia inaequalis. Phytopathology 97, 1222– 1230. doi: 10.1094/PHYTO-97-10-1222, PMID: 18943680 · DOI
  37. Sun Y. Y. Wang Y. Xu Y. Chen T. Li B. Q. Zhang Z. Q.. (2021). Application and mechanism of benzyl-isothiocyanate, a natural antimicrobial agent from cruciferous vegetables, in controlling postharvest decay of strawberry. Postharvest Biol. Technol. 180: 111604. doi: 10.1016/j.postharvbio.2021.111604 · DOI
  38. Tang Y. Li Y. C. Bi Y. Wang Y. (2017). Role of pear fruit cuticular wax and surface hydrophobicity in regulating the prepenetration phase of Alternaria alternata infection. J. Phytopathol. 165, 313– 322. doi: 10.1111/jph.12564 · DOI
  39. Toledo A. V. Franco M. E. E. Lopez S. M. Y. Troncozo M. I. Saparrat M. C. N. Balatti P. A. (2017). Physiological and molecular plant pathology Melanins in fungi: types, localization and putative biological roles. Physiol. Mol. Plant Pathol. 99, 2– 6. doi: 10.1016/j.pmpp.2017.04.004 · DOI
  40. Tsuji G. Kenmochi Y. Takano Y. Sweigard J. Farrall L. Furusawa I.. (2000). Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn(II)2Cys6 binuclear cluster DNA-binding motifs and regulate transcription of melanin biosynthesis genes in a developmentally specific manner. Mol. Microbiol. 38, 940– 954. doi: 10.1046/j.1365-2958.2000.02181.x 11123670 · DOI · Paper page
  41. Wang B. He X. F. Han Z. H. Zong Y. Y. Xue H. L. William O.. (2023). PacC mediated ammonia synthesis contributes to the pathogenicity of Trichothecium roseum by regulating reactive oxygen species and extracellular enzymes. Postharvest Biol. Technol. 201: 112361. doi: 10.1016/j.postharvbio.2023.112361 · DOI
  42. Wang Y. Hu X. Fang Y. Anchieta A. Goldman P. H. Hernandez G.. (2018). Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae. Microbiology 164, 685– 696. doi: 10.1099/mic.0.000633, PMID: 29485393 PMC5982140 · DOI · Paper page
  43. Wang Z. Y. Jenkinson J. M. Holcombe L. J. Soanes D. M. Veneault-Fourrey C. Bhambra G. K.. (2005). The molecular biology of appressorium turgor generation by the rice blast fungus Magnaporthe grisea. Biochem. Soc. Trans. 33, 384– 388. doi: 10.1042/BST0330384 15787612 · DOI
  44. Wang X. L. Lu D. X. Tian C. M. (2021). Analysis of melanin biosynthesis in the plant pathogenic fungus Colletotrichum gloeosporioides. Fungal Biol. 125, 679– 692. doi: 10.1016/j.funbio.2021.04.004, PMID: 34420695 · DOI · Paper page
  45. Wang T. Ren D. D. Guo H. Chen X. Zhu P. K. Nie H. Z.. (2020). CgSCD1 is essential for melanin biosynthesis and pathogenicity of Colletotrichum gloeosporioides. Pathogens 9: 141. doi: 10.3390/pathogens9020141, PMID: 32093195 PMC7169410 · DOI · Paper page
  46. Wang Y. M. Zhou J. Y. Zhong J. Luo D. Li Z. M. Yang J.. (2018). A novel Cys2His2 zincfinger transcription factor Bcaba R1 positively regulates abscisic acid production in Botrytis cinerea. Appl. Environ. Microbiol. 84, 1– 44. doi: 10.1128/AEM.00920-18, PMID: 29959241 PMC6102986 · DOI
  47. Wei W. Xiong Y. Zhu W. Wang N. Yang G. Peng F. (2016). Colletotrichum higginsianum mitogen-activated protein kinase ChMK1: role in growth, cell wall integrity, colony melanization, and pathogenicity. Front. Microbiol. 7: 1212. doi: 10.3389/fmicb.2016.01212 27536296 PMC4971432 · DOI
  48. Xue C. Y. Wu H. Y. Hou M. Y. Ma Q. Z. Guo Y. S. Geng Y. H.. (2022). Functional analysis of the scytalone dehydratase gene CcSCD1 in Corynespora cassiicola from strawberry. Acta Phytopathol. Sin 52, 25– 36. doi: 10.13926/j.cnki.apps.000562 (in Chinese) · DOI
  49. Zhang C. H. He Y. F. Zhu P. K. Chen L. Wang Y. W. Ni B.. (2015). Loss of bcbrn1 and bcpks13 in Botrytis cinerea not only blocks Melanization but also increases vegetative growth and virulence. Mol. Plant Microbe Interact. 28, 1091– 1101. doi: 10.1094/MPMI-04-15-0085-R, PMID: 26035129 · DOI · Paper page
  50. Zhang M. Li Y. C. Wang T. L. Bi Y. Li R. Huang Y.. (2021). AaPKAc regulates differentiation of infection structures induced by physicochemical signals from pear fruit cuticular wax, secondary metabolism, and pathogenicity of Alternaria alternata. Front. Plant Sci. 12: 642601. doi: 10.3389/fpls.2021.642601, PMID: 33968101 PMC8096925 · DOI

Cited by

Sorted by citations

Authors
Li, R., Li, Y., Xu, W., Liu, W., Xu, X., Bi, Y., & Prusky, D.
Affiliations
Gansu Agricultural University (CN)
Corresponding author
Li, Yongcai
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2024-01-11
Volume
14
Pages
1327765-1327765
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2023.1327765
PMC
PMC10808324
OpenAlex
W4390806328
Cited by
17
References
1
Citations by year
2026: 12 · 2025: 4 · 2024: 1
FWCI
3.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China (32372411, 32060567)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
Keywords
Alternaria alternata, scytalone dehydratase, melanogenesis, 1,8-dihydroxynaphthalene, pear black spot, fungal pathogenicity, oxidative stress resistance, hydrogen peroxide, superoxide, spore morphology, infection structures, deletion mutants

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