Cited by 65Neurological Disease Mechanisms and TreatmentsNeurological Disorders and TreatmentsAlzheimer's disease research and treatments

Anti-Inflammatory and Neuroprotective Effects of Co-UltraPEALut in a Mouse Model of Vascular Dementia

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Siracusa, Rosalba, Impellizzeri, Daniela, Cordaro, Marika, Crupi, Rosalia, Esposito, Emanuela, Petrosino, Stefania, Cuzzocrea, Salvatore·2017·Frontiers in Neurology

Abstract

Vascular dementia (VaD), the second most common cause of cognitive impairment in the population, is a disease that results from reduction in regional cerebral blood flow and involves oxidative stress and inflammation. Co-ultramicronized PEALut (co-ultra PEALut) is a new compound with beneficial effects, which include anti-inflammatory and anti-oxidant properties. Recently, co-ultraPEALut has been shown to exhibit neuroprotective effects in models of Parkinson's disease, cerebral ischemia and Alzheimer's disease. However, its effects on VaD remain unknown. Therefore, the purpose of the present study was to highlight the potential neuroprotective actions of co-ultraPEALut containing N-Palmitoylethanolamine (PEA) and the anti-oxidant flavonoid luteolin (Lut) (10:1 by mass) in a mouse model of VaD induced by bilateral carotid arteries occlusion. At 24 hours after VaD induction, mice were orally treated with 1 mg/kg co-ultraPEALut daily for 15 days. On the 15th day, brain tissues were processed for histological, immunohistochemical, western blot and immunofluorescent analysis. Our results clearly demonstrate that co-ultraPEALut improved learning, memory ability, locomotor activity and the reciprocal social interaction. In addition, the mice subjected to VaD and treated with the co-ultraPEALut showed a reorganization of CA1 and CA3 regions of the hippocampus and restored the number of hippocampal neurons as evidenced by NeuN expression, a specific marker of neurons. Furthermore following carotid arteries ligation, mice treated with co-ultraPEALut, showed a modification of pro-inflammatory, pro-apoptotic proteins and of oxidative stress as evidenced by the expression of IκB-α, NF-κB p65, Bax , Bcl-2, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In order, co-ultraPEALut treatment restored VaD-induced loss of brain-derived neurotrophic factor (BDNF) and neurotrophins 3 (NT-3) expression in mice. These results confirmed that the neuroprotective effects of co-ultraPEALut were associated with its anti-inflammatory and anti-oxidant properties.

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Crossref · 10.3389/fneur.2017.00233

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  • Luteolin improves cognitive abilities in a mouse model of vascular dementia by modulating pro-inflammatory and pro-apoptotic proteins and stimulating neurogenesis.

    “luteolin has been demonstrated to improve cognitive abilities in a mouse model of vascular dementia by modulating the expression of pro-inflammatory and pro-apoptotic proteins and by stimulating neurogenesis (Siracusa et al., 2017; Cordaro et al., 2020)”

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Authors
Siracusa, R., Impellizzeri, D., Cordaro, M., Crupi, R., Esposito, E., Petrosino, S., & Cuzzocrea, S.
Affiliations
  • University of Messina (IT)
  • Istituto di Chimica Biomolecolare (IT)
Corresponding author
Cuzzocrea, Salvatore
Venue
Frontiers in Neurology
Published
2017-06-06
Volume
8
Pages
233-233
ISSN
1664-2295 (ISSN-L)
OpenAlex
W2623874973
Cited by
65
References
65
Citations by year
2026: 1 · 2025: 4 · 2024: 8 · 2023: 4 · 2022: 16 · 2021: 10 · 2020: 10 · 2019: 5 · 2018: 6 · 2017: 1
FWCI
3.00
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Neurology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Università degli Studi di Messina
Topics
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Keywords
vascular dementia, N-palmitoylethanolamine, luteolin, neuroprotection, anti-inflammatory activity, oxidative stress, bilateral common carotid artery occlusion, hippocampal neuroprotection, brain-derived neurotrophic factor, neurotrophin-3, NF-κB signaling, apoptosis, learning and memory, social interaction

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