Cited by 167Metabolism, Diabetes, and CancerNatural Antidiabetic Agents StudiesFlavonoids in Medical Research

Quercetin, a Lead Compound against Type 2 Diabetes Ameliorates Glucose Uptake via AMPK Pathway in Skeletal Muscle Cell Line

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Dhanya, R., Arya, A. D., Nisha, P., Jayamurthy, P.·2017·Frontiers in Pharmacology

Abstract

Herein we investigated the molecular mechanism of action of the citrus flavonoid, quercetin in skeletal muscle cells (L6 myotubes). Taking advantage of protein kinase inhibitors, we proved that the effect of quercetin on 2-NBDG uptake in L6 myotubes was not through insulin signaling pathway, but through adenosine monophosphate kinase (AMPK) pathway and its downstream target p38 MAPK. An increase in the cellular AMP to ATP ratio on pretreatment may account for AMPK activation which was coupled with a transient change in mitochondrial membrane potential. In addition, quercetin triggered a rise in intracellular calcium suggesting that calcium-calmodulin mediated protein kinase (CaMKK) may also be involved. Quercetin shared a similar mechanism with the well-known drug metformin, highlighting it as a promising compound for the management of type 2 diabetes. The AMPK signaling pathway could contribute to correction of insulin resistance through bypassing the insulin-regulated system for GLUT4 translocation.

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Authors
Dhanya, R., Arya, A. D., Nisha, P., & Jayamurthy, P.
Affiliations
National Institute for Interdisciplinary Science and Technology (IN)
Corresponding author
Jayamurthy, P.
Venue
Frontiers in Pharmacology
Published
2017-06-08
Volume
8
Pages
336-336
ISSN
1663-9812 (ISSN-L)
OpenAlex
W2617658608
Cited by
167
References
40
Citations by year
2026: 16 · 2025: 23 · 2024: 25 · 2023: 17 · 2022: 23 · 2021: 21 · 2020: 12 · 2019: 17 · 2018: 11 · 2017: 1 · 2012: 1
FWCI
5.03
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Pharmacology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Kerala State Council for Science, Technology and Environment
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
  • Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
Keywords
quercetin, type 2 diabetes, glucose uptake, AMPK signaling, p38 MAPK, CaMKK, L6 myotubes, skeletal muscle, insulin resistance, GLUT4 translocation, metformin, citrus flavonoids, mitochondrial membrane potential, intracellular calcium

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