The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Impact of Biofluid Viscosity on Size and Sedimentation Efficiency of the Isolated Microvesicles
Open accessAbstract
Microvesicles are nano-sized lipid vesicles released by all cells in vivo and in vitro. They are released physiologically under normal conditions but their rate of release is higher under pathological conditions such as tumors. Once released they end up in the systemic circulation and have been found and characterized in all biofluids such as plasma, serum, cerebrospinal fluid, breast milk, ascites, and urine. Microvesicles represent the status of the donor cell they are released from and they are currently under intense investigation as a potential source for disease biomarkers. Currently, the "gold standard" for isolating microvesicles is ultracentrifugation, although alternative techniques such as affinity purification have been explored. Viscosity is the resistance of a fluid to a deforming force by either shear or tensile stress. The different chemical and molecular compositions of biofluids have an effect on its viscosity and this could affect movements of the particles inside the fluid. In this manuscript we addressed the issue of whether viscosity has an effect on sedimentation efficiency of microvesicles using ultracentrifugation. We used different biofluids and spiked them with polystyrene beads and assessed their recovery using the Nanoparticle Tracking Analysis. We demonstrate that MVs recovery inversely correlates with viscosity and as a result, sample dilutions should be considered prior to ultracentrifugation when processing any biofluids.
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Cites
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Cited by
Gurunathan, S., Kang, M.-H., Jeyaraj, M., Qasim, M., & Kim, J.-H. (2019) · Cells · doi:10.3390/cells8040307 · Cited by 1,122
Methodological Guidelines to Study Extracellular Vesicles
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Current methods for the isolation of extracellular vesicles
Momen-Heravi, F., Balaj, L., Alian, S., Mantel, P.-Y., Halleck, A. E., Trachtenberg, A. J., Soria, C. E., Oquin, S., Bonebreak, C. M., Saracoglu, E., et al. (2013) · bchm · doi:10.1515/hsz-2013-0141 · Cited by 578
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Modern isolation and separation techniques for extracellular vesicles
Liangsupree, T., Multia, E., & Riekkola, M.-L. (2021) · Journal of Chromatography A · doi:10.1016/j.chroma.2020.461773 · Cited by 512
Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography
Monguió-Tortajada, M., Gálvez-Montón, C., Bayes-Genis, A., Roura, S., & Borràs, F. E. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03071-y · Cited by 438
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Regulation of exosome production and cargo sorting
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Exosome-mediated delivery of functionally active miRNA-155 inhibitor to macrophages
Momen-Heravi, F., Bala, S., Bukong, T., & Szabo, G. (2014) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2014.03.014 · Cited by 306
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Matrix-Bound Nanovesicles: The Effects of Isolation Method upon Yield, Purity, and Function
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Metadata
- Authors
- Momen-Heravi, F., Balaj, L., Alian, S., Trachtenberg, A. J., Hochberg, F. H., Skog, J., & Kuo, W. P.
- Venue
- Frontiers in Physiology
- Publisher
- Frontiers Media SA
- Published
- 2012
- Volume
- 3
- Pages
- 162-162
- ISSN
- 1664-042X (ISSN-L)
- OpenAlex
- W2116572568
- Cited by
- 257
- References
- 1
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- gold
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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