Exploring the importance of sulfate transporters and ATP sulphurylases for selenium hyperaccumulation—a comparison of Stanleya pinnata and Brassica juncea (Brassicaceae)
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Selenium (Se) hyperaccumulation, the capacity of some species to concentrate Se to levels upwards of 0.1% of dry weight, is an intriguing phenomenon that is only partially understood. Questions that remain to be answered are: do hyperaccumulators have one or more Se-specific transporters? How are these regulated by Se and sulfur (S)? In this study, hyperaccumulator Stanleya pinnata was compared with related non-hyperaccumulator Brassica juncea with respect to S-dependent selenate uptake and translocation, as well as for the expression levels of three sulfate/selenate transporters (Sultr) and three ATP sulphurylases (APS). Selenium accumulation went down ~10-fold with increasing sulfate supply in B. juncea, while S. pinnata only had a 2-3-fold difference in Se uptake between the highest (5 mM) and lowest sulfate (0 mM) treatments. The Se/S ratio was generally higher in the hyperaccumulator than the non-hyperaccumulator, and while tissue Se/S ratio in B. juncea largely reflected the ratio in the growth medium, S. pinnata enriched itself up to 5-fold with Se relative to S. The transcript levels of Sultr1;2 and 2;1 and APS1, 2, and 4 were generally much higher in S. pinnata than B. juncea, and the species showed differential transcript responses to S and Se supply. These results indicate that S. pinnata has at least one transporter with significant selenate specificity over sulfate. Also, the hyperaccumulator has elevated expression levels of several sulfate/selenate transporters and APS enzymes, which likely contribute to the Se hyperaccumulation and hypertolerance phenotype.
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Uptake of Se in the presence of sulfate and phosphate is suppressed.
“Uptake of Se in the presence of sulfate and phosphate is suppressed [34,167,178,179].”
From Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review (2015) · cites this paper as [179]
The Discussion reports that excess sulfate inhibited selenate uptake in S. pinnata, supporting the sulfate portion of the claim under that condition. Our copy does not report suppression of Se uptake by phosphate (searched: phosphate and phosphate-related uptake). No page numbers are available in the indexed passages. (LLM, conf=0.88)
Experimental — accuracy not yet measuredA high-affinity root sulfate transporter was found to be expressed at much higher levels in S. pinnata relative to B. juncea.
“Indeed, a high-affinity root sulfate transporter was found to be expressed at much higher levels in S. pinnata relative to B. juncea [179].”
From Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review (2015) · cites this paper as [179]
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- Authors
- Schiavon, M., Pilon, M., Malagoli, M., & Pilon-Smits, E. A. H.
- Affiliations
- University of Padua (IT)
- Colorado State University (US)
- Corresponding author
- Malagoli, Mario
- Venue
- Frontiers in Plant Science
- Publisher
- Frontiers Media SA
- Published
- 2015-01-23
- Volume
- 6
- Pages
- 2-2
- ISSN
- 1664-462X (ISSN-L)
- PMC
- PMC4304243
- OpenAlex
- W2072921949
- Cited by
- 148
- References
- 48
- Citations by year
- 2026: 10 · 2025: 10 · 2024: 13 · 2023: 9 · 2022: 18 · 2021: 10 · 2020: 30 · 2019: 4 · 2018: 13 · 2017: 12 · 2016: 8 · 2015: 11
- FWCI
- 14.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Plant Science (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
- Padua Research Archive (University of Padova) (submittedVersion, other-oa)
- Funders
- National Science Foundation (1244142)
- U.S. Department of Agriculture (2012-67-13-19416, 2012-67)
- Università degli Studi di Padova
- National Institute of Food and Agriculture (2012‐67)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nitrogen and Sulfur Effects on Brassica
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- selenium hyperaccumulation, Stanleya pinnata, Brassica juncea, ATP sulphurylase, sulfur supply, gene expression, hyperaccumulator plants, Brassicaceae
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