The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
SLM microscopy: scanless two-photon imaging and photostimulation using spatial light modulators
Open accessAbstract
Laser microscopy has generally poor temporal resolution, caused by the serial scanning of each pixel. This is a significant problem for imaging or optically manipulating neural circuits, since neuronal activity is fast. To help surmount this limitation, we have developed a "scanless" microscope that does not contain mechanically moving parts. This microscope uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision. To demonstrate the usefulness of this microscope, we perform two-photon uncaging of glutamate to activate dendritic spines and cortical neurons in brain slices. We also use it to carry out fast (60 Hz) two-photon calcium imaging of action potentials in neuronal populations. Thus, SLM microscopy appears to be a powerful tool for imaging and optically manipulating neurons and neuronal circuits. Moreover, the use of SLMs expands the flexibility of laser microscopy, as it can substitute traditional simple fixed lenses with any calculated lens function.
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Cites
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Hochberg, L. R., Serruya, M. D., Friehs, G. M., Mukand, J. A., Saleh, M., Caplan, A. H., Branner, A., Chen, D., Penn, R. D., & Donoghue, J. P. (2006) · Nature · doi:10.1038/nature04970 · Cited by 3,295
Neural substrates of awakening probed with optogenetic control of hypocretin neurons
Adamantidis, A. R., Zhang, F., Aravanis, A. M., Deisseroth, K., & de Lecea, L. (2007) · Nature · doi:10.1038/nature06310 · Cited by 1,341
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy
Evans, C. L., Potma, E. O., Puoris'haag, M., Côté, D., Lin, C. P., & Xie, X. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0508282102 · Cited by 957
Attractor dynamics of network UP states in the neocortex
Cossart, R., Aronov, D., & Yuste, R. (2003) · Nature · doi:10.1038/nature01614 · Cited by 659
Self-citationYuste, R., & Katz, L. C. (1991) · Neuron · doi:10.1016/0896-6273(91)90243-s · Cited by 591
Highly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy
Hopt, A., & Neher, E. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)76173-5 · Cited by 442
The spine neck filters membrane potentials
Araya, R., Jiang, J., Eisenthal, K. B., & Yuste, R. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608755103 · Cited by 258
Self-citationSodium channels amplify spine potentials
Araya, R., Nikolenko, V., Eisenthal, K. B., & Yuste, R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705282104 · Cited by 80
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Two-photon optogenetic toolbox for fast inhibition, excitation and bistable modulation
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Sapap3 Deletion Anomalously Activates Short-Term Endocannabinoid-Mediated Synaptic Plasticity
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Metadata
- Authors
- Nikolenko, V.
- Venue
- Frontiers in Neural Circuits
- Publisher
- Frontiers Media SA
- Published
- 2008
- Volume
- 2
- Pages
- 5-5
- ISSN
- 1662-5110 (ISSN-L)
- OpenAlex
- W2099865122
- Cited by
- 340
- References
- 8
- FWCI
- 9.34
- Type
- journal article
- Language
- en
- Open access
- gold
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
