Super Critical Fluid Extracted Fatty Acids from Withania somnifera Seeds Repair Psoriasis-Like Skin Lesions and Attenuate Pro-Inflammatory Cytokines (TNF-α and IL-6) Release
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(1) Background: Withania somnifera Dunal (Ashwagandha) is a widely used medicinal herb in traditional medicinal systems with extensive research on various plant parts. Surprisingly, seeds of W. somnifera have never been investigated for their therapeutic potential. (2) Methods: W. somnifera seeds were extracted for fatty acids (WSSO) using super critical fluid extraction, and was analyzed by gas chromatography. Its therapeutic potential in psoriasis-like skin etiologies was investigated using a 12-O tetradecanoyl phorbol 13-acetate (TPA)-induced psoriatic mouse model. Psoriatic inflammation along with psoriatic lesions and histopathological scores were recorded. WSSO was also tested on murine macrophage (RAW264.7), human epidermoid (A431), and monocytic (THP-1) cells, stimulated with TPA or lipo poly-saccharide (LPS) to induce pro-inflammatory cytokine (IL-6 and TNF-α) release. NFκB promoter activity was also measured by luciferase reporter assay. (3) Results: Topical application of WSSO with concurrent oral doses significantly reduced inflammation-induced edema, and repaired psoriatic lesions and associated histopathological scores. Inhibition of pro-inflammatory cytokines release was observed in WSSO-treated A431 and THP-1 cells, along with reduced NFκB expression. WSSO also inhibited reactive nitrogen species (RNS) in LPS-stimulated RAW264.7 cells. (4) Conclusion: Here we show that the fatty acids from W. somnifera seeds have strong anti-inflammatory properties, along with remarkable therapeutic potential on psoriasis-like skin etiologies.
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Fatty acids extracted from W. somnifera seeds have good anti-inflammatory effects and exert an enhanced effect on psoriasis by reducing the release of pro-inflammatory factors (TNF-α and IL-6).
“The fatty acids extracted from W. somnifera seeds have good anti-inflammatory effects and exert an enhanced effect on psoriasis by reducing the release of pro-inflammatory factors (TNF-α and IL–6) [128].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [128]
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Diet and Skin Aging—From the Perspective of Food Nutrition
Cao, C., Xiao, Z., Wu, Y., & Ge, C. (2020) · Nutrients · doi:10.3390/nu12030870 · Cited by 231 · see citing works
- Authors
- Balkrishna, A., Nain, P., Chauhan, A., Sharma, N., Gupta, A., Ranjan, R., & Varshney, A.
- Affiliations
- Patanjali Research Foundation (IN)
- Corresponding author
- Ranjan, Ravikant
- Varshney, Anurag
- Venue
- Biomolecules
- Publisher
- MDPI AG
- Published
- 2020-01-25
- Volume
- 10
- Issue
- 2
- Pages
- 185
- ISSN
- 2218-273X (ISSN-L)
- PMC
- PMC7072271
- OpenAlex
- W3001456491
- Cited by
- 48
- References
- 65
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 8 · 2023: 8 · 2022: 6 · 2021: 9 · 2020: 3
- FWCI
- 7.18
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biomolecules (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Pharmacology › Pharmacological Effects of Medicinal Plants
- Keywords
- Withania somnifera, fatty acids, supercritical fluid extraction, psoriasis, psoriatic inflammation, TNF-α, interleukin-6, NF-κB, anti-inflammatory activity, reactive nitrogen species, topical application, macrophages, keratinocytes
- MeSH terms
- RAW 264.7 Cells, Animals, Disease Models, Animal, Humans, Inflammation, Psoriasis, Seeds, Skin, Tetradecanoylphorbol Acetate, Tumor Necrosis Factor-alpha, Signal Transduction, Interleukin-6, Withania, Mice
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