Comparison of the Chemical and Microbial Composition and Aerobic Stability of High-Moisture Barley Grain Ensiled with Either Chemical or Viable Lactic Acid Bacteria Application
Open accessAbstract
This experiment was aimed at comparing how a chemical additive or an inoculant would affect the dry matter (DM) losses, fermentation pattern, yeast and mold counts, and aerobic stability (AS) of the ensiled high-moisture barley grain (675 g kg−1 DM). Crimped barley grain was ensiled with or without chemical additive AIV Ässä Na and an inoculant SiloSolve FC, totaling three treatments (1 × 3 factorial scheme) for the fermentation periods lasting 7, 14, 28, 60, and 90 days. The application of a chemical additive showed higher pH levels, retained water-soluble carbohydrates (WSCs) better, lowered DM loss, and reduced concentrations of fermentation products. Barley grain treated with chemicals initially showed a marked reduction in yeast and fungal growth and a higher AS up to day 60 of storage but became less effective in later stages of storage, leading to decreased AS. Barley grain inoculated with homo- and heterofermentative strains had decreased silage pH; its WSC content was similar to control and had higher content of weak acids and 1,2 propanediol, reducing mold and yeast counts. With fermentation duration from 28 days and beyond (i.e., 90 days), the inoculant treatment achieved the longest AS and the lowest increase in pH and weight loss during the period of air exposure.
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Temperature dynamics of aerobically exposed silages were continuously recorded following the methodology described by Jatkauskas et al.
“The temperature dynamics of the aerobically exposed silages were continuously recorded throughout this period, following the methodology described by Jatkauskas et al. [38].”
From Influence of Ensiling Timing and Inoculation on Whole Plant Maize Silage Fermentation and Aerobic Stability (Preliminary Research) (2024) · cites this paper as [38]
Section 2.4 (Aerobic Stability Measurements) describes monitoring the temperature of aerobically exposed silage with thermocouple wires and data loggers. It says the loggers recorded temperatures at six-hour intervals, rather than continuously; that frequency does not support the claim’s wording. (LLM, conf=0.86)
Experimental — accuracy not yet measuredChemical and microbial analyses were conducted following the procedures described by Jatkauskas et al.
“Chemical and microbial analyses were conducted following the procedures described by Jatkauskas et al. [38].”
From Influence of Ensiling Timing and Inoculation on Whole Plant Maize Silage Fermentation and Aerobic Stability (Preliminary Research) (2024) · cites this paper as [38]
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Tahir, M., Wang, T., Zhang, J., Xia, T., Deng, X. M., Cao, X., & Zhong, J. (2025) · BMC Microbiology · doi:10.1186/s12866-025-03781-3 · Cited by 23 · see citing works
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“Chemical and microbial analyses were conducted following the procedures described by Jatkauskas et al. [38].”
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Self-citation
- Authors
- Jatkauskas, J., Vrotniakiene, V., Eisner, I., Witt, K. L., & do Amaral, R. C.
- Affiliations
- Lithuanian University of Health Sciences (LT)
- Department of Animal Nutrition and Feedstuffs, Lithuanian University of Health Sciences, R. Žebenkos 12, 82317 Baisogala, Lithuania
- Chr. Hansen (Denmark) (DK)
- Chr. Hansen Holding A/S, Hansen Animal Health and Nutrition, 2970 Hørsholm, Denmark
- Venue
- Fermentation
- Publisher
- MDPI AG
- Published
- 2024-01-16
- Volume
- 10
- Issue
- 1
- Pages
- 62
- ISSN
- 2311-5637 (ISSN-L)
- OpenAlex
- W4390933431
- Cited by
- 6
- References
- 26
- Citations by year
- 2025: 4 · 2024: 2
- FWCI
- 3.03
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Fermentation (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Lithuanian University of Health Sciences (submittedVersion, cc-by)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- silage, lactic acid bacteria inoculant, aerobic stability, dry matter loss, water-soluble carbohydrates, silage pH, yeast and mold counts, homofermentative lactic acid bacteria, heterofermentative lactic acid bacteria, propylene glycol, fermentation products
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