The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian Cancer Invasion and Metastasis
Open accessAbstract
The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay are consistent with cancer cell motility and invasion observed in in vitro assays. Our results demonstrate that the CAM assay is a robust and cost effective model to study ovarian cancer cell metastasis. It is therefore a very useful in vivo model for screening of potential novel therapeutics.
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Cites
Ovarian Cancer Development and Metastasis
Lengyel, E. (2010) · The American Journal of Pathology · doi:10.2353/ajpath.2010.100105 · Cited by 1,672
Deryugina, E. I., & Quigley, J. P. (2008) · Histochemistry and Cell Biology · doi:10.1007/s00418-008-0536-2 · Cited by 279
Ossowski, L. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.6.2437 · Cited by 271
Zhai, Y., Kuick, R., Nan, B., Ota, I., Weiss, S. J., Trimble, C. L., Fearon, E. R., & Cho, K. R. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-2056 · Cited by 249
The Role of Annexin A2 in Tumorigenesis and Cancer Progression
Lokman, N. A., Ween, M. P., Oehler, M. K., & Ricciardelli, C. (2011) · Cancer Microenvironment · doi:10.1007/s12307-011-0064-9 · Cited by 230
Self-citationApigenin inhibits tumor angiogenesis through decreasing HIF-1 and VEGF expression
Fang, J., Zhou, Q., Liu, L.-Z., Xia, C., Hu, X., Shi, X., & Jiang, B.-H. (2006) · Carcinogenesis · doi:10.1093/carcin/bgl205 · Cited by 216
Zijlstra, A., Mellor, R. L., Panzarella, G., Aimes, R. T., Hooper, J. D., Marchenko, N. D., & Quigley, J. P. (2002) · PubMed · Cited by 207
Luo, H., Rankin, G. O., Liu, L., Daddysman, M. K., Jiang, B.-H., & Chen, Y. C. (2009) · Nutrition and Cancer · doi:10.1080/01635580802666281 · Cited by 196
The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials
Valdes, T. I., Kreutzer, D., & Moussy, F. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10152 · Cited by 191
Accessing key steps of human tumor progression in vivo by using an avian embryo model
Hagedorn, M., Javerzat, S., Gilges, D., Meyre, A., de Lafarge, B., Eichmann, A., & Bikfalvi, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408622102 · Cited by 189
Cited by
Giobbe, G. G., Crowley, C., Luni, C., Campinoti, S., Khedr, M., Kretzschmar, K., De Santis, M. M., Zambaiti, E., Michielin, F., Meran, L., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13605-4 · Cited by 500
Allgayer, H., Mahapatra, S., Mishra, B., Swain, B., Saha, S., Khanra, S., Kumari, K., Panda, V. K., Malhotra, D., Patil, N. S., et al. (2025) · Molecular Cancer · doi:10.1186/s12943-025-02338-2 · Cited by 127
Fan, T. M., Roberts, R. D., & Lizardo, M. M. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.00013 · Cited by 90
Ning, J., Lin, Z., Zhao, X., Zhao, B., & Miao, J. (2019) · Cell Death & Disease · doi:10.1038/s41419-019-2095-y · Cited by 28
Konings, G. F., Cornel, K. M., Xanthoulea, S., Delvoux, B., Skowron, M. A., Kooreman, L., Koskimies, P., Krakstad, C., Salvesen, H. B., van Kuijk, K., et al. (2018) · The Journal of Pathology · doi:10.1002/path.5004 · Cited by 26
Metadata
- Authors
- Lokman, N. A., Elder, A. S. F., Ricciardelli, C., & Oehler, M. K.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2012-08-10
- Volume
- 13
- Issue
- 8
- Pages
- 9959-9970
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W1985801265
- Cited by
- 358
- References
- 27
- FWCI
- 13.73
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian Cancer Invasion and Metastasis. https://app.cochat.ai/papers/10.3390/ijms13089959
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- Lokman, N. A., Elder, A. S. F., Ricciardelli, C., & Oehler, M. K. (2012). Chick Chorioallantoic Membrane (CAM) Assay as an In Vivo Model to Study the Effect of Newly Identified Molecules on Ovarian Cancer Invasion and Metastasis. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms13089959
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