Accelerated wound closure in neutrophil-depleted mice
Abstract
The infiltration of neutrophils into injured tissue is known to protect wounds from invading pathogens. However, more recent studies suggest that neutrophils might inhibit the wound repair process. To investigate the role of neutrophils in wounds, mice were neutrophil-depleted by injection with rabbit anti-mouse neutrophil serum. Remarkably, epidermal healing, measured by wound closure, proceeded significantly faster in neutropenic than control mice (77.7+14.2% vs. 41.2+0.9%, P<0.02 at day 2). Dermal healing was not affected by neutrophil depletion, as neither collagen deposition nor wound-breaking strength was significantly different between neutropenic and control mice. As the delayed repair of diabetic individuals exhibits robust inflammation, the effect of neutrophil depletion on diabetic wound healing was investigated. Similar to the observations in wild-type mice, wound closure was accelerated by nearly 50% in neutropenic, diabetic mice. The results suggest that although neutrophils may provide protection against infection, they may retard wound closure.
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Mice depleted of neutrophils using a rabbit antineutrophil serum show faster wound healing than controls.
“By contrast with the CXCR2−/− mice, mice depleted of neutrophils using a rabbit antineutrophil serum show faster wound healing than controls.50”
From The Innate Immune System in Acute and Chronic Wounds (2016) · cites this paper as [50]
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- Authors
- Dovi, J. V., He, L.-K., & DiPietro, L. A.
- Affiliations
- Loyola University Medical Center (US)
- Corresponding author
- DiPietro, Luisa A
- ORCID
- DiPietro, Luisa A
- Venue
- Journal of Leukocyte Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-04-01
- Volume
- 73
- Issue
- 4
- Pages
- 448-455
- ISSN
- 0741-5400 (ISSN-L)
- 1938-3673
- OpenAlex
- W2163105501
- Cited by
- 525
- References
- 28
- Citations by year
- 2026: 6 · 2025: 16 · 2024: 16 · 2023: 28 · 2022: 26 · 2021: 26 · 2020: 22 · 2019: 32 · 2018: 30 · 2017: 39 · 2016: 30 · 2015: 28 · 2014: 33 · 2013: 26 · 2012: 22
- FWCI
- 13.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Leukocyte Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Keywords
- neutrophils, wound healing, neutrophil depletion, skin wound healing, collagen deposition, wound tensile strength, diabetic wound healing, inflammation
- MeSH terms
- Animals, Collagen, Diabetes Mellitus, Epithelial Cells, Female, Flow Cytometry, Macrophages, Mice, Inbred BALB C, Mice, Inbred C57BL, Peroxidase, Neutropenia, Neutrophils, Rabbits, Skin, Wound Healing, Wounds and Injuries, Mice, Knockout, Mice
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