The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Challenges in Exosome Isolation and Analysis in Health and Disease
Open accessAbstract
A growing body of evidence emphasizes the important role exosomes in different physiological and pathological conditions. Exosomes, virus-size extracellular vesicles (EVs), carry a complex molecular cargo, which is actively processed in the endocytic compartment of parental cells. Exosomes carry and deliver this cargo to recipient cells, serving as an intercellular communication system. The methods for recovery of exosomes from supernatants of cell lines or body fluids are not uniformly established. Yet, studies of the quality and quantity of exosome cargos underlie the concept of “liquid biopsy.” Exosomes are emerging as a potentially useful diagnostic tool and a predictor of disease progression, response to therapy and overall survival. Although many novel approaches to exosome isolation and analysis of their cargos have been introduced, the role of exosomes as diagnostic or prognostic biomarkers of disease remains unconfirmed. This review considers existing challenges to exosome validation as disease biomarkers. Focusing on advantages and limitations of methods for exosome isolation and characterization, approaches are proposed to facilitate further progress in the development of exosomes as biomarkers in human disease.
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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
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Optimized exosome isolation protocol for cell culture supernatant and human plasma
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“Due to the endosomal origin, exosomes are enriched in late endosome components such as CD63, CD9 and CD81 [3].”
BackgroundIsolation of Extracellular Vesicles: General Methodologies and Latest Trends
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Cited by
Exosome Processing and Characterization Approaches for Research and Technology Development
Lai, J. J., Chau, Z. L., Chen, S., Hill, J. J., Korpany, K. V., Liang, N., Lin, L., Lin, Y., Liu, J. K., Liu, Y., et al. (2022) · Advanced Science · doi:10.1002/advs.202103222 · Cited by 623
Technologies and Standardization in Research on Extracellular Vesicles
Gandham, S., Su, X., Wood, J., Nocera, A. L., Alli, S. C., Milane, L., Zimmerman, A., Amiji, M., & Ivanov, A. R. (2020) · Trends in Biotechnology · doi:10.1016/j.tibtech.2020.05.012 · Cited by 494
Chronic inflammation and the hallmarks of aging
Baechle, J. J., Chen, N., Makhijani, P., Winer, S., Furman, D., & Winer, D. A. (2023) · Molecular Metabolism · doi:10.1016/j.molmet.2023.101755 · Cited by 471
Jia, Y., Yu, L., Ma, T., Xu, W., Qian, H., Sun, Y., & Shi, H. (2022) · Theranostics · doi:10.7150/thno.74305 · Cited by 391
Tsiapalis, D., & O’Driscoll, L. (2020) · Cells · doi:10.3390/cells9040991 · Cited by 313
Metadata
- Authors
- Ludwig, N., Whiteside, T. L., & Reichert, T. E.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2019-09-21
- Volume
- 20
- Issue
- 19
- Pages
- 4684
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W2973748748
- Cited by
- 428
- References
- 65
- FWCI
- 13.89
- Type
- journal article
- Language
- en
- Open access
- gold
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