Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Cui, Yanhua; Miao, Kai; Niyaphorn, Siripitakyotin; Qu, Xiaojun

doi:10.3390/ijms21030995

Cited by 451GABA and Rice ResearchProbiotics and Fermented FoodsMicrobial Metabolic Engineering and Bioproduction

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

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Cui, Yanhua, Miao, Kai, Niyaphorn, Siripitakyotin, Qu, Xiaojun·2020·International Journal of Molecular Sciences

DOI 10.3390/ijms21030995PMC PMC7037312

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Abstract

Gamma-aminobutyric acid (GABA) is widely distributed in nature and considered a potent bioactive compound with numerous and important physiological functions, such as anti-hypertensive and antidepressant activities. There is an ever-growing demand for GABA production in recent years. Lactic acid bacteria (LAB) are one of the most important GABA producers because of their food-grade nature and potential of producing GABA-rich functional foods directly. In this paper, the GABA-producing LAB species, the biosynthesis pathway of GABA by LAB, and the research progress of glutamate decarboxylase (GAD), the key enzyme of GABA biosynthesis, were reviewed. Furthermore, GABA production enhancement strategies are reviewed, from optimization of culture conditions and genetic engineering to physiology-oriented engineering approaches and co-culture methods. The advances in both the molecular mechanisms of GABA biosynthesis and the technologies of synthetic biology and genetic engineering will promote GABA production of LAB to meet people’s demand for GABA. The aim of the review is to provide an insight of microbial engineering for improved production of GABA by LAB in the future.

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The pH is a key factor for GABA biosynthesis by LAB and affects not only bacterial growth but also the activity of GAD.
“The pH is a key factor for GABA biosynthesis by LAB and affects not only bacterial growth but also the activity of GAD [272].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [272]
GABA is the major inhibitory neurotransmitter of the central nervous system.
“GABA is the major inhibitory neurotransmitter of the central nervous system [269,272,273,274].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [272]

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Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry
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Authors: Cui, Y., Miao, K., Niyaphorn, S., & Qu, X.
Affiliations: Harbin Institute of Technology (CN)
Heilongjiang Academy of Sciences (CN)
Corresponding author: Cui, Yanhua
ORCID: Cui, Yanhua
Venue: International Journal of Molecular Sciences
Publisher: MDPI AG
Published: 2020-02-03
Volume: 21
Issue: 3
Pages: 995
ISSN: 1422-0067 (ISSN-L)
DOI: 10.3390/ijms21030995
PMC: PMC7037312
OpenAlex: W3004119871
Cited by: 451
References: 115
Citations by year: 2026: 66 · 2025: 81 · 2024: 79 · 2023: 88 · 2022: 61 · 2021: 56 · 2020: 19 · 2017: 1
FWCI: 4.72
Type: journal article
Language: en
Open access: gold
dx.doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: International Journal of Molecular Sciences (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders: National Natural Science Foundation of China (31471712 and 31371827)
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › GABA and Rice Research
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: GABA, lactic acid bacteria, GABA synthesis, glutamic acid decarboxylase, microbial engineering, co-culture, culture condition optimization, functional foods, antihypertensive effect, antidepressant effects
MeSH terms: gamma-Aminobutyric Acid, Genetic Engineering, Lactobacillales

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Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

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