Probiogenomic Analysis of Lactiplantibacillus plantarum SPS109: A Potential GABA-Producing and Cholesterol-Lowering Probiotic Strain
Open accessAbstract
Lactiplantibacillus plantarum SPS109, an isolated strain of lactic acid bacteria (LAB) from fermented foods, showed remarkable potential as a probiotic with dual capabilities in γ-aminobutyric acid (GABA) production and cholesterol reduction. This study employs genomic and comparative analyses to search into the strain's genetic profile, safety features, and probiotic attributes. The safety assessment reveals the absence of virulence factors and antimicrobial resistance genes, while the genome uncovers bacteriocin-related elements, including sactipeptides and a cluster for putative plantaricins, strengthening its ability to combat diverse pathogens. Pangenome analysis revealed unique bacteriocin-related genes, specifically lcnD and bcrA , distinguishing SPS109 from four other L. plantarum strains producing GABA. In addition, genomic study emphasizes SPS109 strain distinctive features, two GABA-related genes responsible for GABA production and a bile tolerance gene ( cbh ) crucial for cholesterol reduction. Additionally, the analysis highlights several genes of potential probiotic properties, including stress tolerance, vitamin production, and antioxidant activity. In summary, L. plantarum SPS109 emerges as a promising probiotic candidate with versatile applications in the food and beverage industries, supported by its unique genomic features and safety profile.
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Studies have suggested a set of genes associated with resistance to stress, active metabolism in the host, adhesion, and immunomodulation as genes with probiotic properties.
“Studies have suggested a set of genes associated with resistance to stress, active metabolism in the host, adhesion, and immunomodulation as genes with probiotic properties [37,38,39].”
From Genomic Characterization of Lactiplantibacillus plantarum Strains: Potential Probiotics from Ethiopian Traditional Fermented Cottage Cheese (2024) · cites this paper as [37]
The Probiotic marker genes section (Table 2; page unavailable) identifies genes for stress tolerance, adhesion, immunomodulation, and gut persistence as markers of probiotic potential. It does not identify “active metabolism in the host” as a marker category or establish that the listed genes are active in the host. (LLM, conf=0.78)
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Han, X., Liu, Q., Li, Y., Zhang, M., Liu, K., Kwok, L.-Y., Zhang, H., & Zhang, W. (2025) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2025.104938 · Cited by 64 · see citing works
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“…set of genes associated with resistance to stress, active metabolism in the host, adhesion, and immunomodulation as genes with probiotic properties [37–39] . of the four strains evaluated in this work ( L. plantarum 54B, 54C, 55A, and WCFS1). expected, most of the core genome ‘probiotic marker…”
InfluentialLee, Y.-S., Noh, D.-I., Lee, S.-J., Jeon, M.-H., Kim, Y.-R., Jang, W. J., & Lee, E.-W. (2025) · Journal of Applied Microbiology · doi:10.1093/jambio/lxaf256 · Cited by 3 · see citing works
- Authors
- Chintakovid, N., Singkhamanan, K., Yaikhan, T., Nokchan, N., Wonglapsuwan, M., Jitpakdee, J., Kantachote, D., & Surachat, K.
- Affiliations
- Prince of Songkla University (TH)
- Corresponding author
- Surachat, Komwit
- ORCID
- Venue
- Heliyon
- Publisher
- Elsevier BV
- Published
- 2024-07
- Volume
- 10
- Issue
- 13
- Pages
- e33823
- ISSN
- 2405-8440 (ISSN-L)
- PMC
- PMC11263657
- OpenAlex
- W4400131474
- Cited by
- 26
- References
- 53
- Citations by year
- 2026: 9 · 2025: 15 · 2024: 2
- FWCI
- 5.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Heliyon (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Prince of Songkla University (MED6701023S, B13F660074)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Plant Science › GABA and Rice Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- probiotic potential, GABA production, cholesterol lowering, bile tolerance, bacteriocins, plantaricin, BCRA, pangenome analysis, virulence factors, antimicrobial resistance genes, stress tolerance, fermented foods
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