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Synthesis of γ-Aminobutyric Acid by Lactic Acid Bacteria Isolated from a Variety of Italian Cheeses

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Siragusa, S., De Angelis, M., Di Cagno, R., Rizzello, C. G., Coda, R., Gobbetti, M.·2007·Applied and Environmental Microbiology
DOI 10.1128/aem.01064-07PMC PMC2168214
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Abstract

The concentrations of γ-aminobutyric acid (GABA) in 22 Italian cheese varieties that differ in several technological traits markedly varied from 0.26 to 391 mg kg −1 . Presumptive lactic acid bacteria were isolated from each cheese variety (total of 440 isolates) and screened for the capacity to synthesize GABA. Only 61 isolates showed this activity and were identified by partial sequencing of the 16S rRNA gene. Twelve species were found. Lactobacillus paracasei PF6, Lactobacillus delbrueckii subsp. bulgaricus PR1, Lactococcus lactis PU1, Lactobacillus plantarum C48, and Lactobacillus brevis PM17 were the best GABA-producing strains during fermentation of reconstituted skimmed milk. Except for L. plantarum C48, all these strains were isolated from cheeses with the highest concentrations of GABA. A core fragment of glutamate decarboxylase (GAD) DNA was isolated from L. paracasei PF6, L. delbrueckii subsp. bulgaricus PR1, L. lactis PU1, and L. plantarum C48 by using primers based on two highly conserved regions of GAD. A PCR product of ca. 540 bp was found for all the strains. The amino acid sequences deduced from nucleotide sequence analysis showed 98, 99, 90, and 85% identity to GadB of L. plantarum WCFS1 for L. paracasei PF6, L. delbrueckii subsp. bulgaricus PR1, L. lactis PU1, and L. plantarum C48, respectively. Except for L. lactis PU1, the three lactobacillus strains survived and synthesized GABA under simulated gastrointestinal conditions. The findings of this study provide a potential basis for exploiting selected cheese-related lactobacilli to develop health-promoting dairy products enriched in GABA.

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  • Escherichia spp. and Lactobacillus spp. have been demonstrated to synthesize GABA.

    “Escherichia spp.(Richard and Foster, 2003) and Lactobacillus spp.(Siragusa et al., 2007) have been demonstrated to synthesize GABA.”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Siragusa et al., 2007

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Authors
Siragusa, S., De Angelis, M., Di Cagno, R., Rizzello, C. G., Coda, R., & Gobbetti, M.
Affiliations
  • University of Bari Aldo Moro (IT)
  • Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, Bari 70126, Italy
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2007-11-15
Volume
73
Issue
22
Pages
7283-7290
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.01064-07
PMC
PMC2168214
OpenAlex
W2127666176
Cited by
438
References
3
Citations by year
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FWCI
12.56
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
  • CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
  • Open Research (University of Surrey) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › GABA and Rice Research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
Keywords
GABA, GABA synthesis, lactic acid bacteria, Italian cheese, glutamic acid decarboxylase, gadB, Lactobacillus paracasei, Lactobacillus bulgaricus, Lactococcus lactis, Lactobacillus plantarum, Lactobacillus brevis, gastrointestinal survival, 16S rRNA identification
MeSH terms
Amino Acid Sequence, Cheese, Dairy Products, DNA, Bacterial, Food Microbiology, gamma-Aminobutyric Acid, Genotype, Glutamate Decarboxylase, Italy, Lactobacillus, Molecular Sequence Data, RNA, Ribosomal, 16S, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Lactobacillus plantarum, Lactiplantibacillus plantarum, Lactobacillus delbrueckii, Levilactobacillus brevis

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