Gut Organoid as a New Platform to Study Alginate and Chitosan Mediated PLGA Nanoparticles for Drug Delivery
Open accessAbstract
Intestinal organoids can be used as an ex vivo epithelial model to study different drug delivery effects on epithelial cells’ luminal surface. In this study, the impact of surface charge on the delivery of 5-ASA loaded PLGA nanoparticles into the lumen of organoids was investigated. Alginate and chitosan were used to coat the nanoparticles and provide negative and positive charges on the particles, respectively. The organoid growth and viability were not affected by the presence of either alginate- or chitosan-coated nanoparticles. It was shown that nanoparticles could be transported from the serosal side of the organoids to the lumen as the dye gradually accumulated in the lumen by day 2–3 after adding the nanoparticles to the Matrigel. By day 5, the dye was eliminated from the lumen of the organoids. It was concluded that the positively charged nanoparticles were more readily transported across the epithelium into the lumen. It may be attributed to the affinity of epithelial cells to the positive charge. Thus, the organoid can be utilized as an appropriate model to mimic the functions of the intestinal epithelium and can be used as a model to evaluate the benefits of nanoparticle-based drug delivery.
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Growth factors can be encapsulated within nanoparticles and conjugated onto cell surfaces for controlled release.
“these growth factors can be encapsulated within nanoparticles, and conjugated onto cell surfaces for controlled release85–88.”
From Organoids (2022) · cites this paper as [87]
The Introduction describes encapsulating drugs in nanoparticles and prolonged drug release. The Materials and Methods (section 4.2) and Conclusions identify 5-ASA—not growth factors—as the nanoparticle cargo. Although growth factors are listed as materials (section 4.1), the paper does not say they were encapsulated or conjugated onto cell surfaces for controlled release; page numbers were not available in our copy. (LLM, conf=0.82)
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- Authors
- Davoudi, Z., Peroutka-Bigus, N., Bellaire, B., Jergens, A., Wannemuehler, M., & Wang, Q.
- Affiliations
- Iowa State University (US)
- Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011, USA
- Corresponding author
- Wang, Qun
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2021-05-20
- Volume
- 19
- Issue
- 5
- Pages
- 282
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC8161322
- OpenAlex
- W3161315229
- Cited by
- 84
- References
- 52
- Citations by year
- 2026: 8 · 2025: 17 · 2024: 12 · 2023: 24 · 2022: 19 · 2021: 4
- FWCI
- 3.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Iowa State University Digital Repository (Iowa State University) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Pharmaceutical Research and Manufacturers of America Foundation (RSGTMT17)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Drug Solubulity and Delivery Systems
- Keywords
- intestinal organoids, PLGA nanoparticles, alginate coating, chitosan coating, surface charge, 5-aminosalicylic acid, intestinal epithelium, nanoparticle drug delivery
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Alginates, Aminosalicylic Acids, Animals, Cell Survival, Collagen, Drug Combinations, Intestine, Small, Laminin, Microscopy, Microscopy, Fluorescence, Organoids, Particle Size, Proteoglycans, Drug Delivery Systems, Cell Death, Microscopy, Confocal, Chitosan, Mice, Nanoparticles, In Vitro Techniques
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