Novel Antioxidant Peptides from Crassostrea Hongkongensis Improve Photo-Oxidation in UV-Induced HaCaT Cells
Open accessAbstract
Enzymatic hydrolysates from Oysters (OAH) display multiple biological activities. Previously, a 3~5 KDa oyster ultrafiltration component (OUP) showed a high property of preventing skin oxidation. Subsequently, we identified specific peptides with such activity. OUP was fractionated stepwise by Sephadex-G25 and RP-HPLC, and active fractions were screened using UV-irradiated HaCaT cells. The most active fractions (OP5-3) were analyzed by LC-MS/MS and a total of 17 peptides were identified. Results from mass spectrometry showed that OP5-3 consisted of peptides with a molecular weight range of 841.51–1786.92 Da. Six of these peptides were synthesized for validating the activity of resisting skin oxidation in the same cell model. All six peptides showed varying degrees of antioxidant activity, while pretreatment of HaCaT cells with AIVAEVNEAAK alleviated UV cytotoxicity, inhibited metalloproteinase 1 (MMP-1) expression, and showed the highest activity to resist UV-induced skin photo-oxidation among these peptides. In addition, results from molecular docking analysis of MMP-1 with AIVAEVNEAAK showed that AIVAEVNEAAK suppresses its enzymatic activity by directly interacting with MMP-1 and thus exhibit anti-photoaging activity.
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Zhang et al. found that peptides derived from Crassostrea Hongkongensis improved cell viability and reduced oxidative stress markers in UV-exposed HaCaT cells.
“The researchers found that these novel antioxidant peptides were able to improve cell viability and reduce oxidative stress markers in UV‐exposed cells, indicating their potential as protective agents against photodamage.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [55]
Zhang et al.'s study has relatively low level of evidence because it is an in vitro study on HaCaT cells.
“However, the level of evidence for this study is relatively low according to the Oxford Centre for Evidence‐Based Medicine's hierarchy of evidence, as it is an in vitro study conducted on HaCaT cells.”
From Cosmeceuticals in photoaging: A review. (2024) · cites this paper as [55]
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Cites
Cellular senescence: from physiology to pathology
Muñoz-Espín, D., & Serrano, M. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3823 · Cited by 2,818 · see citing works
Hallmarks of Cellular Senescence
Hernandez-Segura, A., Nehme, J., & Demaria, M. (2018) · Trends in Cell Biology · doi:10.1016/j.tcb.2018.02.001 · Cited by 2,605 · see citing works
The oyster genome reveals stress adaptation and complexity of shell formation
Zhang, G., Fang, X., Guo, X., Li, L., Luo, R., Xu, F., Yang, P., Zhang, L., Wang, X., Qi, H., et al. (2012) · Nature · doi:10.1038/nature11413 · Cited by 2,241 · see citing works
Self-citationCellular Senescence: Aging, Cancer, and Injury
Calcinotto, A., Kohli, J., Zagato, E., Pellegrini, L., Demaria, M., & Alimonti, A. (2019) · Physiological Reviews · doi:10.1152/physrev.00020.2018 · Cited by 1,270 · see citing works
Senescence and aging: Causes, consequences, and therapeutic avenues
McHugh, D., & Gil, J. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201708092 · Cited by 1,233 · see citing works
Oxidation events and skin aging
Kammeyer, A., & Luiten, R. M. (2015) · Ageing Research Reviews · doi:10.1016/j.arr.2015.01.001 · Cited by 980 · see citing works
Molecular Mechanisms of Dermal Aging and Antiaging Approaches
Shin, J.-W., Kwon, S.-H., Choi, J.-Y., Na, J.-I., Huh, C.-H., Choi, H.-R., & Park, K.-C. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20092126 · Cited by 875 · see citing works
Photoageing: mechanism, prevention and therapy
Yaar, M., & Gilchrest, B. A. (2007) · British Journal of Dermatology · doi:10.1111/j.1365-2133.2007.08108.x · Cited by 755 · see citing works
Matrix metalloproteinase interactions with collagen and elastin
Doren, S. R. V. (2015) · Matrix Biology · doi:10.1016/j.matbio.2015.01.005 · Cited by 463 · see citing works
Sunlight damage to cellular DNA: focus on oxidatively generated lesions
Schuch, A. P., Moreno, N. C., Schuch, N. J., Menck, C. F. M., & Garcia, C. C. M. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.01.029 · Cited by 436 · see citing works
Stem cell competition orchestrates skin homeostasis and ageing
Liu, N., Matsumura, H., Kato, T., Ichinose, S., Takada, A., Namiki, T., Asakawa, K., Morinaga, H., Mohri, Y., De Arcangelis, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1085-7 · Cited by 422 · see citing works
Oxygen toxicity: chemistry and biology of reactive oxygen species
Buonocore, G., Perrone, S., & Tataranno, M. L. (2010) · Seminars in Fetal and Neonatal Medicine · doi:10.1016/j.siny.2010.04.003 · Cited by 321 · see citing works
Aging, Cell Senescence, and Chronic Disease: Emerging Therapeutic Strategies
Tchkonia, T., & Kirkland, J. L. (2018) · JAMA · doi:10.1001/jama.2018.12440 · Cited by 302 · see citing works
Vayalil, P. K., Mittal, A., Hara, Y., Elmets, C. A., & Katiyar, S. K. (2004) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2004.22622.x · Cited by 262 · see citing works
Tumor cell escape from therapy-induced senescence
Saleh, T., Tyutyunyk-Massey, L., Murray, G. F., Alotaibi, M. R., Kawale, A. S., Elsayed, Z., Henderson, S. C., Yakovlev, V., Elmore, L. W., Toor, A., et al. (2019) · Biochemical Pharmacology · doi:10.1016/j.bcp.2018.12.013 · Cited by 182 · see citing works
Cell Senescence, DNA Damage, and Metabolism
Shmulevich, R., & Krizhanovsky, V. (2021) · Antioxidants & Redox Signaling · doi:10.1089/ars.2020.8043 · Cited by 157 · see citing works
Roles and Functions of ROS and RNS in Cellular Physiology and Pathology
Žarković, N. (2020) · Cells · doi:10.3390/cells9030767 · Cited by 131 · see citing works
Novel antioxidative peptides from the protein hydrolysate of oysters (Crassostrea talienwhanensis)
Wang, Q., Li, W., He, Y., Ren, D., Kow, F., Song, L., & Yu, X. (2013) · Food Chemistry · doi:10.1016/j.foodchem.2013.08.099 · Cited by 105 · see citing works
Kostyuk, V., Potapovich, A., Albuhaydar, A. R., Mayer, W., De Luca, C., & Korkina, L. (2018) · Rejuvenation Research · doi:10.1089/rej.2017.1931 · Cited by 85 · see citing works
Hwang, D., Kang, M., Jo, M. J., et al. (2019) · Marine Drugs · doi:10.3390/md17020129 · Cited by 84 · see citing works
Xiang, X., Zheng, H., Wang, R., Chen, H., Xiao, J., Zheng, B., Liu, S., & Ding, Y. (2021) · Marine Drugs · doi:10.3390/md19080456 · Cited by 84 · see citing works
p16INK4a reporter mice reveal age-promoting effects of environmental toxicants
Sorrentino, J. A., Krishnamurthy, J., Tilley, S. L., Alb, J. G., Burd, C. E., & Sharpless, N. E. (2013) · Journal of Clinical Investigation · doi:10.1172/jci70960 · Cited by 82 · see citing works
Antioxidant and anti‐inflammatory peptide fraction from oyster soft tissue by enzymatic hydrolysis
Qian, B., Zhao, X., Ye, Y., et al. (2020) · Food Science & Nutrition · doi:10.1002/fsn3.1710 · Cited by 75 · see citing works
Peng, Z., Chen, B., Zheng, Q., Zhu, G., Cao, W., Qin, X., & Zhang, C. (2020) · Marine Drugs · doi:10.3390/md18060288 · Cited by 60 · see citing works
Self-citationFuda, H., Watanabe, M., Hui, S., Joko, S., Okabe, H., Jin, S., Takeda, S., Miki, E., Watanabe, T., & Chiba, H. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.12.066 · Cited by 58 · see citing works
Redirecting drug repositioning to discover innovative cosmeceuticals
Sotiropoulou, G., Zingkou, E., & Pampalakis, G. (2021) · Experimental Dermatology · doi:10.1111/exd.14299 · Cited by 50 · see citing works
Siregar, A. S., Nyiramana, M. M., Kim, E., Cho, S. B., Woo, M. S., Lee, D., Hong, S.-G., Han, J., Kang, S. S., Kim, D. R., et al. (2021) · Marine Drugs · doi:10.3390/md19110614 · Cited by 45 · see citing works
Li, Y., Qiu, W., Zhang, Z., Han, X., Bu, G., Meng, F., Kong, F., Cao, X., Huang, A., Feng, Z., et al. (2019) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.9997 · Cited by 39 · see citing works
Preparation and antioxidant activities of oligosaccharides from Crassostrea gigas
Wu, S., & Huang, X. (2016) · Food Chemistry · doi:10.1016/j.foodchem.2016.08.043 · Cited by 37 · see citing works
Xiang, N., Zhao, C., Diao, X., Han, Q., & Zhou, H. (2017) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2017.07.009 · Cited by 35 · see citing works
Han, J.-H., Bang, J. S., Choi, Y. J., & Choung, S. (2018) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2018.09.036 · Cited by 33 · see citing works
Williams, D. E., & Andersen, R. J. (2019) · Natural Product Reports · doi:10.1039/c9np00054b · Cited by 31 · see citing works
de Laat, J., van der A, R. J., Allaart, M., van Weele, M., Benítez, G. C., Casiccia, C. C., Leme, N. M. P., Quel, E. J., Salvador, J., & Wolfram, E. A. (2010) · Geophysical Research Letters · doi:10.1029/2010gl043699 · Cited by 28 · see citing works
The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii
Zhang, H., & He, M. (2020) · Scientific Reports · doi:10.1038/s41598-019-57329-3 · Cited by 25 · see citing works
Siregar, A. S., Nyiramana, M. M., Kim, E., Shin, E., Woo, M. S., Kim, J.-M., Kim, J. H., Lee, D., Hahm, J. R., Kim, H. J., et al. (2020) · Marine Drugs · doi:10.3390/md18100512 · Cited by 20 · see citing works
Zhang, X., Peng, Z., Zheng, H., et al. (2021) · Marine Drugs · doi:10.3390/md19100566 · Cited by 19 · see citing works
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Marine Bioactive Peptides: Anti-Photoaging Mechanisms and Potential Skin Protective Effects
Zhang, X., Zhuang, H., Wu, S., Mao, C., Dai, Y., & Yan, H. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46020063 · Cited by 45 · see citing works
The Anti-Photoaging Activity of Peptides from Pinctada martensii Meat
Wei, M., Qiu, H., Zhou, J., Yang, C., Chen, Y., & You, L. (2022) · Marine Drugs · doi:10.3390/md20120770 · Cited by 33 · see citing works
Cosmeceuticals in photoaging: A review.
Chan, L. K. W., Lee, K. W. A., Lee, C. H., Lam, K. W. P., Lee, K. F. V., Wu, R., Wan, J., Shivananjappa, S., Sky, W. T. H., Choi, H., et al. (2024) · Skin Research and Technology · doi:10.1111/srt.13730 · Cited by 23 · see citing works
- Authors
- Zhang, C., Lv, J., Qin, X., Peng, Z., & Lin, H.
- Affiliations
- Guangdong Ocean University (CN)
- Corresponding author
- Qin, Xiaoming
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2022-01-24
- Volume
- 20
- Issue
- 2
- Pages
- 100
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC8875629
- OpenAlex
- W4210274382
- Cited by
- 24
- References
- 36
- Citations by year
- 2026: 5 · 2025: 1 · 2024: 10 · 2023: 5 · 2022: 3
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- This research was financially supported by the China Agricultural Research System of MOF and MARA, High Value Aquatic Products Processing and Utilization Innovation Team of Higher Education Institutions of Guangdong Province (GDOU2016030503). (GDOU2016030503)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- antioxidant peptides, Crassostrea hongkongensis, HaCaT keratinocytes, MMP-1 inhibition, anti-photoaging, peptide synthesis, molecular docking
- MeSH terms
- HaCaT Cells, Animals, Antioxidants, Chromatography, Liquid, Humans, Oxidation-Reduction, Peptides, Skin, Ultraviolet Rays, Skin Aging, Crassostrea, Tandem Mass Spectrometry, Molecular Docking Simulation
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