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Validation of a Vitamin D Specific Questionnaire to Determine Vitamin D Status in Athletes
Abstract
The study objective was to validate a food frequency and lifestyle questionnaire (FFLQ) to assess vitamin D intake and lifestyle factors affecting status. Methods: Data collected previously during the fall (n = 86), winter (n = 49), and spring (n = 67) in collegiate-athletes (Study 1) and in active adults (n = 123) (Study 2) were utilized. Study 1: Vitamin D intake and ultraviolet B exposure were estimated using the FFLQ and compared to serum 25(OH)D concentrations via simple correlation and linear regression modeling. Study 2: Vitamin D intake from food was estimated using FFLQ and compared to vitamin D intake reported in 7-Day food diaries via paired t-test and Bland–Altman analysis. Results: Study 1: Serum 25(OH)D was not associated with vitamin D intake from food, food plus supplements, or sun exposure, but was associated with tanning bed use (r = 0.39) in spring, supplement use in fall (r = 0.28), and BMI (body mass index) (r = −0.32 to −0.47) across all seasons. Serum 25(OH)D concentrations were explained by BMI, tanning bed use, and sun exposure in fall, (R = 0.42), BMI in winter (R = 0.32), and BMI and tanning bed use in spring (R = 0.52). Study 2: Estimated Vitamin D intake from food was 186.4 ± 125.7 via FFLQ and 148.5 ± 228.2 IU/day via food diary. There was no association between intake estimated by the two methodologies (r = 0.12, p < 0.05). Conclusions: FFLQ-estimated vitamin D intake was not associated with serum 25(OH)D concentration or food-record-estimated vitamin D intake. Results highlight the difficulty of designing/utilizing intake methodologies for vitamin D, as its status is influenced by body size and both endogenous and exogenous (dietary) sources.
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“This finding aligns with existing evidence indicating that vitamin D deficiency is prevalent in athletic populations (23–25).”
From The decline of vitamin D in adolescent athletes: insights from a one-year follow-up and its association with electrocardiographic changes (2025) · cites this paper as [23]
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“This finding aligns with existing evidence indicating that vitamin D deficiency is prevalent in athletic populations (23–25).”
From The decline of vitamin D in adolescent athletes: insights from a one-year follow-up and its association with electrocardiographic changes (2025) · cites this paper as [23]
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Vitamin D for health: a global perspective
Hossein-nezhad, A., & Holick, M. F. (2013) · Mayo Clinic Proceedings · doi:10.1016/j.mayocp.2013.05.011 · Cited by 1,298
Food-frequency questionnaires: a review of their design, validation and utilisation
Cade, J. E., Burley, V. J., Warm, D. L., Thompson, R. L., & Margetts, B. M. (2004) · Nutrition Research Reviews · doi:10.1079/nrr200370 · Cited by 518 · cited 1×
“A review focusing on the design, utilization and validation of FFQs, however, found that FFQs that are interviewer-administered and which allow subjects to report their own portion sizes are more likely to yield a correlation with their reference method [36].”
BackgroundVitamin D bioavailability: state of the art
Borel, P., Caillaud, D., & Cano, N. J. (2015) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2012.688897 · Cited by 378
The effect of body composition and BMI on 25(OH)D response in vitamin D‐supplemented athletes
Cassity, E. P., Redzic, M., Teager, C. R., & Thomas, D. T. (2016) · European Journal of Sport Science · doi:10.1080/17461391.2015.1125952 · Cited by 21
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Cited by
Ramadan, M. M., Jaber, A. A., Aladwani, A. J., Alayyaf, A. E., Sayed-Noor, A. S., Ghanem, H. B., El-Sherbiny, M., Ebrahim, H. A., Ahmed, M. M., & Elsherbini, D. M. A. (2025) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2025.1731919 · Cited by 1
Metadata
- Authors
- Larson-Meyer, D. E., Douglas, C. S., Thomas, J. J., Johnson, E. C., Barcal, J. N., Heller, J. E., Hollis, B. W., & Halliday, T. M.
- Affiliations
- Department of Family and Consumer Sciences, University of Wyoming, Laramie, WY 82071, USA
- University of Minnesota Athletics, University of Minnesota, Minneapolis, MN 55455, USA
- Division of Kinesiology & Health, University of Wyoming, Laramie, WY 82017, USA
- IMG Academy, Bradenton, FL 34210, USA
- Wellness Department, Arizona State University, Tempe, AZ 85287, USA
- Dr Bruce Hollis’ Laboratory, Medical University of South Carolina, Charleston, SC 29425, USA
- Department of Health, Kinesiology, and Recreation, University of Utah, Salt Lake City, UT 84112, USA
- ORCID
- Thomas, Joi J.
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2019-11-11
- Volume
- 11
- Issue
- 11
- Pages
- 2732
- ISSN
- 2072-6643
- Cited by
- 21
- References
- 4
- Language
- en
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