Cited by 39Muscle Physiology and DisordersNutrition and Health in AgingMuscle metabolism and nutrition

Camphene Attenuates Skeletal Muscle Atrophy by Regulating Oxidative Stress and Lipid Metabolism in Rats

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Baek, Suji, Kim, Jisu, Moon, Byung Seok, Park, Sun Mi, Jung, Da Eun, Kang, Seo Young, Lee, Sang Ju, Oh, Seung Jun +6 more·2020·Nutrients

Abstract

Sarcopenia- or cachexia-related muscle atrophy is due to imbalanced energy metabolism and oxidative stress-induced muscle dysfunction. Monoterpenes play biological and pharmacological reactive oxygen species (ROS) scavenging roles. Hence, we explored the effects of camphene, a bicyclic monoterpene, on skeletal muscle atrophy in vitro and in vivo. We treated L6 myoblast cells with camphene and then examined the ROS-related oxidative stress using Mito TrackerTM Red FM and anti-8-oxoguanine antibody staining. To investigate lipid metabolism, we performed real-time polymerase chain reactions, holotomographic microscopy, and respiratory gas analysis. Rat muscle atrophy in in vivo models was observed using 18F-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography and immunocytochemistry. Camphene reversed the aberrant cell size and muscle morphology of L6 myoblasts under starvation and in in vivo models. Camphene also attenuated E3 ubiquitin ligase muscle RING-finger protein-1, mitochondrial fission, and 8-oxoguanine nuclear expression in starved myotubes and hydrogen peroxide (H2O2)-treated cells. Moreover, camphene significantly regulated lipid metabolism in H2O2-treated cells and in vivo models. These findings suggest that camphene may potentially affect skeletal muscle atrophy by regulating oxidative stress and lipid metabolism.

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Crossref · 10.3390/nu12123731

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  • Camphene reduces starvation-induced oxidative stress and atrophy by regulating ROS generation and lipid metabolism in L6 skeletal muscle cells and SD rats.

    “Camphene, a cyclic monoterpene found in conifers, reduces starvation-induced oxidative stress and atrophy by regulating ROS generation and lipid metabolism in L6 skeletal muscle cells and SD rats [153].”

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Authors
Baek, S., Kim, J., Moon, B. S., Park, S. M., Jung, D. E., Kang, S. Y., Lee, S. J., Oh, S. J., Kwon, S. H., Nam, M. H., Kim, H. O., Yoon, H. J., Kim, B. S., & Lee, K. P.
Affiliations
  • Research and Development Center, UMUST R&D Corporation, Seoul 05029, Korea
  • Konkuk University (KR)
  • Ewha Womans University (KR)
  • Asan Medical Center (KR)
  • Korea Basic Science Institute (KR)
Corresponding author
  • Moon, Byung Seok
  • Kim, Bom Sahn
  • Lee, Kang Pa
Venue
Nutrients
Publisher
MDPI AG
Published
2020-12-03
Volume
12
Issue
12
Pages
3731
ISSN
2072-6643 (ISSN-L)
OpenAlex
W3110268745
Cited by
39
References
38
Citations by year
2026: 5 · 2025: 5 · 2024: 16 · 2023: 9 · 2022: 2 · 2021: 2
FWCI
1.05
Type
journal article
Language
en
Open access
Locations
  • Nutrients (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Konkuk University (KU Research Professor Program)
  • Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science and ICT (2018R1D1A1A02085705, 2018R1D1A1B07045321)
  • Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare (HI18C2383)
  • Korea Basic Science Institute (KBSI) under the R&D programs supervised by the Ministry of Science and ICT (D010710)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
camphene, skeletal muscle atrophy, sarcopenia, cachexia, oxidative stress, lipid metabolism, reactive oxygen species, monoterpenes, mitochondrial fission, 8-oxoguanine, hydrogen peroxide, starvation, L6 myoblasts
MeSH terms
Bicyclic Monoterpenes, Animals, Cachexia, Cell Survival, Disease Models, Animal, Hydrogen Peroxide, Male, Muscular Atrophy, Rats, Sprague-Dawley, Reactive Oxygen Species, Oxidative Stress, Muscle, Skeletal, Muscle Fibers, Skeletal, Myoblasts, Ubiquitin-Protein Ligases, Lipid Metabolism, Rats

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