Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity
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This study was designed to better understand the molecular mechanisms involved in the anabolic resistance observed in elderly people. Nine young (22 ± 0.1 years) and 10 older (69 ± 1.7 years) volunteers performed a one-leg extension exercise consisting of 10 × 10 repetitions at 70% of their 3-RM, immediately after which they ingested 30 g of whey protein. Muscle biopsies were taken from the vastus lateralis at rest in the fasted state and 30 min after protein ingestion in the non-exercised (Pro) and exercised (Pro+ex) legs. Plasma insulin levels were determined at the same time points. No age difference was measured in fasting insulin levels but the older subjects had a 50% higher concentration than the young subjects in the fed state (p < 0.05). While no difference was observed in the fasted state, in response to exercise and protein ingestion, the phosphorylation state of PKB (p < 0.05 in Pro and Pro+ex) and S6K1 (p = 0.059 in Pro; p = 0.066 in Pro+ex) was lower in the older subjects compared with the young subjects. After Pro+ex, REDD1 expression tended to be higher (p = 0.087) in the older group while AMPK phosphorylation was not modified by any condition. In conclusion, we show that the activation of the mTORC1 pathway is reduced in skeletal muscle of older subjects after resistance exercise and protein ingestion compared with young subjects, which could be partially due to an increased expression of REDD1 and an impaired anabolic sensitivity.
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The fractional synthetic rate is lower in healthy elderly individuals compared with young peers.
“despite the lower fractional synthetic rate in healthy elderly individuals compared with young peers [78,79,80]”
From Effects of Protein Supplementation Combined with Exercise Intervention on Frailty Indices, Body Composition, and Physical Function in Frail Older Adults (2018) · cites this paper as [80]
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- Authors
- Francaux, M., Demeulder, B., Naslain, D., Fortin, R., Lutz, O., Caty, G., & Deldicque, L.
- Affiliations
- UCLouvain (BE)
- Institute of Neuroscience, Université catholique de Louvain, Louvain-la-Neuve 1348, Belgium
- Corresponding author
- Francaux, Marc
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2016-01-15
- Volume
- 8
- Issue
- 1
- Pages
- 47
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC4728660
- OpenAlex
- W2233696250
- Cited by
- 83
- References
- 55
- Citations by year
- 2026: 9 · 2025: 9 · 2024: 7 · 2023: 6 · 2022: 6 · 2021: 10 · 2020: 13 · 2019: 7 · 2018: 6 · 2017: 4 · 2016: 6
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Digital Access to Libraries (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Keywords
- aging, mTORC1 signaling, resistance training, REDD1, skeletal muscle anabolism, anabolic resistance, Akt phosphorylation, S6K1 phosphorylation, AMPK, insulin, older adults, vastus lateralis
- MeSH terms
- Whey Proteins, Mechanistic Target of Rapamycin Complex 1, Aged, Aging, Fasting, Humans, Insulin, Phosphorylation, Transcription Factors, Signal Transduction, Exercise, Anabolic Agents, Multiprotein Complexes, Proto-Oncogene Proteins c-akt, Quadriceps Muscle, Resistance Training, AMP-Activated Protein Kinases, Young Adult, TOR Serine-Threonine Kinases
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