The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Exosomal ITGA3 interferes with non‐cancerous prostate cell functions and is increased in urine exosomes of metastatic prostate cancer patients
Open accessAbstract
Background: Cancer cells are able to change the protein expression and behavior of non‐cancerous surrounding cells. Exosomes, secreted by prostate cancer (PCa) cells, may have a functional role in cancer metastasis and present a promising source for protein biomarkers. The aim of the present study was to identify which proteins in exosomes can influence non‐cancerous cells, and to determine whether we can use urine exosomal proteins to identify high‐risk PCa patients. Method: Exosomes were isolated by ultracentrifugation. Migration and invasion were studied by the transwell (invasion) assay. Proteomics was performed by LC‐MS/MS and identified proteins were validated by Western blotting. Cellular uptake of fluorescent labeled PKH67‐exosomes was measured by FACS. Results: Based on comparative protein profiling by mass spectrometry‐based proteomics of LNCaP‐ and PC3‐exosomes, we selected ITGA3 and ITGB1, involved in migration/invasion, for further analyses. Inhibition of exosomal ITGA3 reduced the migration and invasion of non‐cancerous prostate epithelial cells (prEC) almost completely. Cellular uptake of exosomes by prEC was higher with PC3‐exosomes compared to LNCaP exosomes. Finally, ITGA3 and ITGB1 were more abundant in urine exosomes of metastatic patients (p<0.05), compared to benign prostate hyperplasia or PCa. Conclusion: These data indicate exosomal ITGA3 and ITGB1 may play a role in manipulating non‐cancerous surrounding cells and that measurement of ITGA3 and ITGB1 in urine exosomes has the potential to identify patients with metastatic PCa in a non‐invasive manner.
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Ratajczak, J., Wysoczynski, M., Hayek, F., Janowska-Wieczorek, A., & Ratajczak, M. Z. (2006) · Leukemia · doi:10.1038/sj.leu.2404296 · Cited by 1,340
Exosomal Secretion of Cytoplasmic Prostate Cancer Xenograft-derived Proteins
Jansen, F. H., Krijgsveld, J., van Rijswijk, A., van den Bemd, G.-J., van den Berg, M. S., van Weerden, W. M., Willemsen, R., Dekker, L. J., Luider, T. M., & Jenster, G. (2009) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m800443-mcp200 · Cited by 107
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Nontemplated Nucleotide Additions Distinguish the Small RNA Composition in Cells from Exosomes
Koppers-Lalic, D., Hackenberg, M., Bijnsdorp, I. V., van Eijndhoven, M. A. J., Sadek, P., Sie, D., Zini, N., Middeldorp, J. M., Ylstra, B., de Menezes, R. X., et al. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.08.027 · Cited by 560
Self-citationEvidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
Fais, S., O’Driscoll, L., Borras, F. E., Buzas, E., Camussi, G., Cappello, F., Carvalho, J., Cordeiro da Silva, A., Del Portillo, H., El Andaloussi, S., et al. (2016) · ACS Nano · doi:10.1021/acsnano.5b08015 · Cited by 481
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
Self-citationRamirez-Garrastacho, M., Bajo-Santos, C., Line, A., Martens-Uzunova, E. S., de la Fuente, J. M., Moros, M., Soekmadji, C., Tasken, K. A., & Llorente, A. (2022) · British Journal of Cancer · doi:10.1038/s41416-021-01610-8 · Cited by 130
Metadata
- Authors
- Bijnsdorp, I. V., Geldof, A. A., Lavaei, M., Piersma, S. R., van Moorselaar, R. J. A., & Jimenez, C. R.
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2013-01
- Volume
- 2
- Issue
- 1
- ISSN
- 2001-3078 (ISSN-L)
- OpenAlex
- W2091677705
- Cited by
- 167
- References
- 2
- FWCI
- 5.24
- Type
- journal article
- Language
- en
- Open access
- gold
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- Bijnsdorp, I. V., Geldof, A. A., Lavaei, M., Piersma, S. R., van Moorselaar, R. J. A., & Jimenez, C. R. (2013). Exosomal ITGA3 interferes with non‐cancerous prostate cell functions and is increased in urine exosomes of metastatic prostate cancer patients. Journal of Extracellular Vesicles. https://doi.org/10.3402/jev.v2i0.22097
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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