Cited by 107Phytochemicals and Antioxidant ActivitiesAntioxidant Activity and Oxidative StressNatural Products and Biological Research

Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats

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Ko, Sang-Heui, Park, Jae-Hee, Kim, So-Yun, Lee, Seon Woo, Chun, Soon-Sil, Park, Eunju·2014·Preventive Nutrition and Food Science

Abstract

Increased consumption of fresh vegetables that are high in polyphenols has been associated with a reduced risk of oxidative stress-induced disease. The present study aimed to evaluate the antioxidant effects of spinach in vitro and in vivo in hyperlipidemic rats. For measurement of in vitro antioxidant activity, spinach was subjected to hot water extraction (WE) or ethanol extraction (EE) and examined for total polyphenol content (TPC), oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and antigenotoxic activity. The in vivo antioxidant activity of spinach was assessed using blood and liver lipid profiles and antioxidant status in rats fed a high fat-cholesterol diet (HFCD) for 6 weeks. The TPC of WE and EE were shown as 1.5±0.0 and 0.5±0.0 mg GAE/g, respectively. Increasing the concentration of the extracts resulted in increased ORAC value, CAA, and antigenotoxic activity for all extracts tested. HFCD-fed rats displayed hyperlipidemia and increased oxidative stress, as indicated by a significant rise in blood and liver lipid profiles, an increase in plasma conjugated diene concentration, an increase in liver thiobarbituric acid reactive substances (TBARS) level, and a significant decrease in manganese superoxide dismutase (Mn-SOD) activity compared with rats fed normal diet. However, administration of 5% spinach showed a beneficial effect in HFCD rats, as indicated by decreased liver TBARS level and DNA damage in leukocyte and increased plasma conjugated dienes and Mn-SOD activity. Thus, the antioxidant activity of spinach may be an effective way to ameliorate high fat and cholesterol diet-induced oxidative stress.

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Crossref · 10.3746/pnf.2014.19.1.019

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  • Spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage.

    “These results show that spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage [156,162,164,165,166,167,168,169,170].”

    From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [169]

    The Conclusions and Discussion support antioxidant activity and protection against oxidative-stress-related DNA damage in rats. The Results (Effects of spinach on blood and hepatic lipid profiles; page not provided) report no difference in measured blood or liver lipids between spinach-supplemented and unsupplemented high-fat-diet rats, so they do not support a lipid-lowering effect. The paper does not address appetite regulation or tissue regeneration. (LLM, conf=0.90)

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Authors
Ko, S.-H., Park, J.-H., Kim, S.-Y., Lee, S. W., Chun, S.-S., & Park, E.
Affiliations
  • Sunchon National University (KR)
  • Kyungnam University (KR)
Corresponding author
Park, Eunju
Venue
Preventive Nutrition and Food Science
Published
2014-03-31
Volume
19
Issue
1
Pages
19-26
ISSN
  • 1226-332X (ISSN-L)
  • 2287-1098
OpenAlex
W1996240666
Cited by
107
References
45
Citations by year
2026: 5 · 2025: 11 · 2024: 11 · 2023: 8 · 2022: 12 · 2021: 12 · 2020: 9 · 2019: 13 · 2018: 10 · 2017: 5 · 2016: 6 · 2015: 4 · 2014: 1
FWCI
3.74
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Preventive Nutrition and Food Science (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Surgery › Natural Products and Biological Research
Keywords
spinach, hyperlipidemia, antioxidant activity, oxidative stress, high-fat high-cholesterol diet, polyphenols, total polyphenols, ORAC, cellular antioxidant activity, antigenotoxicity, lipid peroxidation, TBARS, superoxide dismutase, DNA damage, dietary supplementation

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