Restraint stress induces and exacerbates intestinal inflammation in interleukin-10 deficient mice
Open accessAbstract
AIM: To investigate the effects of restraint stress on chronic colitis in interleukin (IL)-10 deficient (IL-10(-/-)) mice. METHODS: The first experiment compared the effect of restraint stress on the development of intestinal inflammation in wild-type and IL-10(-/-) mice. Both wild-type and IL-10(-/-) mice were physically restrained in a well-ventilated, 50 cm(3) conical polypropylene tube for 2 h per day for three consecutive days. The second experiment was performed to assess the effect of restraint stress on exacerbation of colitis induced by piroxicam in IL-10(-/-) mice. The IL-10(-/-) mice were exposed to restraint stress for 2 h per day for 3 consecutive days, and then treated with piroxicam for 4 d at a dose of 200 ppm administered in the rodent chow. RESULTS: In the first experiment, none of the wild-type mice with or without restraint stress showed clinical and histopathological abnormality in the gut. However, IL-10(-/-) mice exposed to restraint stress exhibited histologically significant intestinal inflammation as compared to those without restraint stress. In the second experiment, restraint stress significantly reduced body weight and increased the severity of intestinal inflammation assessed by histopathologic grading in IL-10(-/-) mice. Colonic IL12p40 mRNA expression was strongly increased in mice exposed to restraint stress. CONCLUSION: This novel animal model could be useful in future study of psychological stress in the pathogenesis of inflammatory bowel disease.
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Constraint stress aggravates spontaneous colitis in IL-10−/− mice compared to those without stress.
“Constraint stress has been reported to aggravate spontaneous colitis in IL-10−/− mice compared to those without stress (100).”
From Stress triggers flare of inflammatory bowel disease in children and adults (2019) · cites this paper as [100]
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Stress triggers flare of inflammatory bowel disease in children and adults
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- Authors
- Koh, S.-J.
- Affiliations
- Seoul National University (KR)
- Corresponding author
- Koh, Seong-Joon
- ORCID
- Koh, Seong-Joon
- Venue
- World Journal of Gastroenterology
- Publisher
- Baishideng Publishing Group Inc.
- Published
- 2015
- Volume
- 21
- Issue
- 28
- Pages
- 8580
- ISSN
- 1007-9327 (ISSN-L)
- PMC
- PMC4515839
- OpenAlex
- W2410371459
- Cited by
- 14
- References
- 34
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2021: 1 · 2020: 3 · 2019: 3 · 2017: 1 · 2016: 1
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- World Journal of Gastroenterology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- Seoul National University
- National Research Foundation of Korea
- Seoul National University Hospital
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Keywords
- restraint stress, psychological stress, intestinal inflammation, interleukin-10 deficiency, IL-10 knockout mice, chronic colitis, inflammatory bowel disease, animal models
- MeSH terms
- Animals, Chronic Disease, Colitis, Colon, Disease Models, Animal, Gene Expression Regulation, Male, Mice, Inbred C57BL, Piroxicam, Restraint, Physical, RNA, Messenger, Stress, Psychological, Time Factors, Interleukin-10, Mice, Knockout, Receptors, Interleukin-12
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