Cited by 475Mesenchymal stem cell researchPluripotent Stem Cells ResearchHematopoietic Stem Cell Transplantation

Spheroid Culture of Mesenchymal Stem Cells

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Cesarz, Zoe, Tamama, Kenichi·2016·Stem Cells International

Abstract

Compared with traditional 2D adherent cell culture, 3D spheroidal cell aggregates, or spheroids, are regarded as more physiological, and this technique has been exploited in the field of oncology, stem cell biology, and tissue engineering. Mesenchymal stem cells (MSCs) cultured in spheroids have enhanced anti‐inflammatory, angiogenic, and tissue reparative/regenerative effects with improved cell survival after transplantation. Cytoskeletal reorganization and drastic changes in cell morphology in MSC spheroids indicate a major difference in mechanophysical properties compared with 2D culture. Enhanced multidifferentiation potential, upregulated expression of pluripotency marker genes, and delayed replicative senescence indicate enhanced stemness in MSC spheroids. Furthermore, spheroid formation causes drastic changes in the gene expression profile of MSC in microarray analyses. In spite of these significant changes, underlying molecular mechanisms and signaling pathways triggering and sustaining these changes are largely unknown.

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  • Spheroid cell culture has been developed for research on cancer, stem cells, and many organs, including liver, kidney, and heart.

    “This approach has been developed in the research of cancer (7, 76, 102), stem cells (17, 120), and many organs (such as liver, kidney, heart, etc.) (3,16,64,75).”

    From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [17]

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Authors
Cesarz, Z., & Tamama, K.
Affiliations
University of Pittsburgh (US)
Corresponding author
Tamama, Kenichi
Venue
Stem Cells International
Publisher
Wiley
Published
2015-11-16
Volume
2016
Issue
1
Pages
9176357-9176357
ISSN
  • 1687-966X (ISSN-L)
  • 1687-9678
OpenAlex
W1603093856
Cited by
475
References
141
Citations by year
2026: 22 · 2025: 32 · 2024: 46 · 2023: 51 · 2022: 53 · 2021: 85 · 2020: 55 · 2019: 37 · 2018: 45 · 2017: 38 · 2016: 10 · 2015: 1
FWCI
4.40
Type
journal article
Language
en
Open access
Locations
  • Stem Cells International (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
University of Pittsburgh
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Keywords
mesenchymal stem cells, spheroid culture, 3D cell aggregates, stemness, stem cell markers, replicative senescence, gene expression profiling, cell morphology, angiogenesis, tissue regeneration, cell transplantation

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