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Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
Open accessAbstract
Exosomes are nano-sized extracellular vesicles secreted by body fluids and are known to represent the characteristics of cells that secrete them. The contents and morphology of the secreted vesicles reflect cell behavior or physiological status, for example cell growth, migration, cleavage, and death. The exosomes' role may depend highly on size, and the size of exosomes varies from 30 to 300 nm. The most widely used method for exosome imaging is negative staining, while other results are based on Cryo-Transmission Electron Microscopy, Scanning Electron Microscopy, and Atomic Force Microscopy. The typical exosome's morphology assessed through negative staining is a cup-shape, but further details are not yet clear. An exosome well-characterized through structural study is necessary particular in medical and pharmaceutical fields. Therefore, function-dependent morphology should be verified by electron microscopy techniques such as labeling a specific protein in the detailed structure of exosome. To observe detailed structure, ultrathin sectioned images and negative stained images of exosomes were compared. In this protocol, we suggest transmission electron microscopy for the imaging of exosomes including negative staining, whole mount immuno-staining, block preparation, thin section, and immuno-gold labelling.
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Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Extracellular vesicles: Exosomes, microvesicles, and friends
Raposo, G., & Stoorvogel, W. (2013) · Journal of Cell Biology · doi:10.1083/jcb.201211138 · Cited by 8,099
Biological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Membrane vesicles as conveyors of immune responses
Théry, C., Ostrowski, M., & Segura, E. (2009) · Nature Reviews Immunology · doi:10.1038/nri2567 · Cited by 3,882
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., et al. (2015) · Nature · doi:10.1038/nature14581 · Cited by 2,880
Lötvall, J., Hill, A. F., Hochberg, F., Buzás, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., et al. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.26913 · Cited by 2,451
Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles
György, B., Szabó, T. G., Pásztói, M., Pál, Z., Misják, P., Aradi, B., László, V., Pállinger, É., Pap, E., Kittel, Á., et al. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0689-3 · Cited by 2,074
Vlassov, A. V., Magdaleno, S., Setterquist, R., & Conrad, R. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.03.017 · Cited by 1,930
Optimized exosome isolation protocol for cell culture supernatant and human plasma
Lobb, R. J., Becker, M., Wen Wen, S., Wong, C. S. F., Wiegmans, A. P., Leimgruber, A., & Möller, A. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27031 · Cited by 1,705
Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study
Zaborowski, M. P., Balaj, L., Breakefield, X. O., & Lai, C. P. (2015) · BioScience · doi:10.1093/biosci/biv084 · Cited by 1,268
Exosomes/microvesicles as a mechanism of cell-to-cell communication
Camussi, G., Deregibus, M. C., Bruno, S., Cantaluppi, V., & Biancone, L. (2010) · Kidney International · doi:10.1038/ki.2010.278 · Cited by 1,178
Exosomes: Therapy delivery tools and biomarkers of diseases
Barile, L., & Vassalli, G. (2017) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.02.020 · Cited by 1,038
Sokolova, V., Ludwig, A.-K., Hornung, S., Rotan, O., Horn, P. A., Epple, M., & Giebel, B. (2011) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2011.05.013 · Cited by 851
Sorting it out: Regulation of exosome loading
Villarroya-Beltri, C., Baixauli, F., Gutiérrez-Vázquez, C., Sánchez-Madrid, F., & Mittelbrunn, M. (2014) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2014.04.009 · Cited by 742
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes
Conde-Vancells, J., Rodriguez-Suarez, E., Embade, N., Gil, D., Matthiesen, R., Valle, M., Elortza, F., Lu, S. C., Mato, J. M., & Falcon-Perez, J. M. (2008) · Journal of Proteome Research · doi:10.1021/pr8004887 · Cited by 642
Trehalose prevents aggregation of exosomes and cryodamage
Bosch, S., de Beaurepaire, L., Allard, M., Mosser, M., Heichette, C., Chrétien, D., Jegou, D., & Bach, J.-M. (2016) · Scientific Reports · doi:10.1038/srep36162 · Cited by 393
Sharma, S., Rasool, H. I., Palanisamy, V., Mathisen, C., Schmidt, M., Wong, D. T., & Gimzewski, J. K. (2010) · ACS Nano · doi:10.1021/nn901824n · Cited by 383
Wu, Y., Deng, W., & Klinke II, D. J. (2015) · The Analyst · doi:10.1039/c5an00688k · Cited by 369
Tumor Shedding and Coagulation
Dvorak, H. F., Quay, S. C., Orenstein, N. S., Dvorak, A. M., Hahn, P., Bitzer, A. M., & Carvalho, A. C. (1981) · Science · doi:10.1126/science.7195067 · Cited by 302
Kadiu, I., Narayanasamy, P., Dash, P. K., Zhang, W., & Gendelman, H. E. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1102244 · Cited by 231
Exosomes mediate cell contact–independent ephrin-Eph signaling during axon guidance
Gong, J., Körner, R., Gaitanos, L., & Klein, R. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201601085 · Cited by 140
Exosome secretion by eosinophils: A possible role in asthma pathogenesis
Mazzeo, C., Cañas, J. A., Zafra, M. P., Marco, A. R., Fernández‐Nieto, M., Sanz, V., Mittelbrunn, M., Izquierdo, M., Baixauli, F., Sastre, J., et al. (2015) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2014.11.026 · Cited by 134
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells
Kim, S. B., Kim, H., Park, M., Cho, S., Goughnour, P. C., Han, D., Yoon, I., Kim, Y., Kang, T., Song, E., et al. (2017) · The Journal of Cell Biology · doi:10.1083/jcb.201605118 · Cited by 96
Self-citationZaharie, F. V., Muresan, M.-S., Petrushev, B., Berce, C., Gafencu, G., Selicean, S. E., Jurj, A. M., Cojocneanu, R., Lisencu, C. I., Pop, L., et al. (2015) · Journal of Gastrointestinal and Liver Diseases · doi:10.15403/jgld.2014.1121.244.375 · Cited by 93
Observations on cell lines derived from a patient with Hodgkin's disease.
Friend, C., Marovitz, W. F., Henie, G., Henie, W., Tsuei, D., Hirschhorn, K., Holland, J. G., & Cuttner, J. (1978) · PubMed · Cited by 64
Extracellular vesicles – A promising avenue for the detection and treatment of infectious diseases?
Fuhrmann, G., Neuer, A. L., & Herrmann, I. K. (2017) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2017.04.005 · Cited by 59
Identification and analysis of exosomes secreted from macrophages extracted by different methods.
Wang, J., Yao, Y., Wu, J., & Li, G. (2015) · Europe PMC (PubMed Central) · Cited by 50
II. Intestinal epithelial cell exosomes: perspectives on their structure and function
van Niel, G., & Heyman, M. (2002) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00102.2002 · Cited by 45
Liu, X., & Wang, H. (2011) · Journal of Visualized Experiments · doi:10.3791/2574 · Cited by 35
Cited by
Hu, H., Dong, L., Bu, Z., Shen, Y., Luo, J., Zhang, H., Zhao, S., Lv, F., & Liu, Z. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2020.1778883 · Cited by 310
Cao, J., Wang, B., Tang, T., Lv, L., Ding, Z., Li, Z., Hu, R., Wei, Q., Shen, A., Fu, Y., et al. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-01719-2 · Cited by 264
Biogenesis, Isolation, and Detection of Exosomes and Their Potential in Therapeutics and Diagnostics
Sonbhadra, S., Mehak, & Pandey, L. M. (2023) · Biosensors · doi:10.3390/bios13080802 · Cited by 97
Circulating exosomes in cardiovascular disease: Novel carriers of biological information
Liu, Q., Piao, H., Wang, Y., Zheng, D., & Wang, W. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.111148 · Cited by 70
Ryu, I. S., Kim, D. H., Ro, J.-Y., Park, B.-G., Kim, S. H., Im, J.-Y., Lee, J.-Y., Yoon, S. J., Kang, H., Iwatsubo, T., et al. (2023) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2023.110603 · Cited by 45
Metadata
- Authors
- Jung, M. K., & Mun, J. Y.
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2018-01-04
- Issue
- 131
- ISSN
- 1940-087X (ISSN-L)
- OpenAlex
- W2782295218
- Cited by
- 285
- References
- 30
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- bronze
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