Cited by 77Neuroinflammation and Neurodegeneration MechanismsNeuroscience and Neuropharmacology ResearchNeurological Disease Mechanisms and Treatments

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

González Ibanez, Fernando, Picard, Katherine, Bordeleau, Maude, Sharma, Kaushik, Bisht, Kanchan, Tremblay, Marie-Ève·2019·Journal of Visualized Experiments

Abstract

This is a protocol for the dual visualization of microglia and infiltrating macrophages in mouse brain tissue. TMEM119 (which labels microglia selectively), when combined with IBA1 (which provides an exceptional visualization of their morphology), allows investigation of changes in density, distribution, and morphology. Quantifying these parameters is important in providing insights into the roles exerted by microglia, the resident macrophages of the brain. Under normal physiological conditions, microglia are regularly distributed in a mosaic-like pattern and present a small soma with ramified processes. Nevertheless, as a response to environmental factors (i.e., trauma, infection, disease, or injury), microglial density, distribution, and morphology are altered in various manners, depending on the insult. Additionally, the described double-staining method allows visualization of infiltrating macrophages in the brain based on their expression of IBA1 and without colocalization with TMEM119. This approach thus allows discrimination between microglia and infiltrating macrophages, which is required to provide functional insights into their distinct involvement in brain homeostasis across various contexts of health and disease. This protocol integrates the latest findings in neuroimmunology that pertain to the identification of selective markers. It also serves as a useful tool for both experienced neuroimmunologists and researchers seeking to integrate neuroimmunology into proje…

Integrity signals

Registry

Crossref · 10.3791/60510

Cites

22 of 22 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
González Ibanez, F., Picard, K., Bordeleau, M., Sharma, K., Bisht, K., & Tremblay, M.-È.
Affiliations
  • Université Laval (CA)
  • Centre de recherche du CHU de Québec-Université Laval (CA)
Venue
Journal of Visualized Experiments
Published
2019-10-27
Issue
152
ISSN
1940-087X (ISSN-L)
OpenAlex
W2981952575
Cited by
77
References
22
Citations by year
2026: 10 · 2025: 11 · 2024: 12 · 2023: 14 · 2022: 12 · 2021: 10 · 2020: 8
FWCI
3.30
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Visualized Experiments (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
microglia, TMEM119, Iba1, infiltrating macrophages, microglial density, microglial morphology, mouse brain, neuroimmunology, brain homeostasis, microglial activation
MeSH terms
Animals, Brain, Fluorescent Antibody Technique, Homeostasis, Macrophages, Staining and Labeling, Microglia, Myeloid Cells, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review