Development of a High-Performance Liquid Chromatographic Method for Determination of Letrozole in Wistar Rat Serum and its Application in Pharmacokinetic Studies
Open accessAbstract
A fast, sensitive, and specific reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the determination of letrozole in Wistar rat serum was developed. In this method, liquid-liquid extraction of letrozole was achieved using diethyl ether as the extracting solvent. The analysis was carried out on a reversed-phase C18 (250 mm × 4.6 mm, 5 μm) column with an isocratic mobile phase of methanol-water (70:30,v/v), at a flow rate of 1.0 mL min(-1). Detection was carried out at 239 nm with a UV-visible spectrophoto-metric detector. The method was shown to be selective and linear over the concentration range of 0.15-100 μg mL(-1). The intra-day and inter-day precision studies showed good reproducibility with coefficients of variation less than 11% for the analyte. The relative errors of intra- and inter-day accuracy were within -11.52 to -2.26%. The limit of quantification was evaluated to be 0.15 μg mL(-1). The method was successfully applied for the pharmacokinetic study of letrozole after oral administration of 10 mg kg(-1) of letrozole in six healthy Wistar rats.
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Acharjya et al.'s method had a shorter retention time with observable interference corresponding to drug retention time.
“However, the method reported a shorter retention time with observable interference corresponding to drug retention time.”
From A simple, precise, and sensitive HPLC method for quantification of letrozole in rat plasma: development, validation, and preclinical pharmacokinetics (2021) · cites this paper as Acharjya et al. 2012
The Method validation—Specificity section reports a short letrozole retention time of 3.58 ± 0.009 minutes, but explicitly says there were no interfering peaks at the analyte’s location. The claim’s assertion of observable interference at the drug retention time is contrary to that finding. (LLM, conf=0.88)
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredThe drug recovery is comparable with previously reported methods.
“The drug recovery is comparable with the methods reported previously (Acharjya et al. 2012; AlShehri et al. 2019).”
From A simple, precise, and sensitive HPLC method for quantification of letrozole in rat plasma: development, validation, and preclinical pharmacokinetics (2021) · cites this paper as Acharjya et al. 2012
The sensitivity of the present method is considerably more than previous reports where LLOQ was observed at 0.15 μg/mL and 0.1 μg/mL.
“The sensitivity of the present method is considerably more compared to previous reports where LLOQ was observed at 0.15 μ g/mL and 0.1 μ g/mL (Acharjya et al. 2012; Dhakne et al. 2020).”
From A simple, precise, and sensitive HPLC method for quantification of letrozole in rat plasma: development, validation, and preclinical pharmacokinetics (2021) · cites this paper as Acharjya et al. 2012
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Cites
Phase I study of the oral nonsteroidal aromatase inhibitor CGS 20267 in healthy postmenopausal women
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“The reported UV-spectrophotometric methods [4–7] are not sensitive enough to estimate low concentrations of letrozole in biological fluids.”
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Flagged first, then by citations
Research works printed but not linked: 16
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Sorted by citations
Hegde, A. R., Padya, B. S., Soman, S., & Mutalik, S. (2021) · Journal of Analytical Science and Technology · doi:10.1186/s40543-021-00276-4 · Cited by 17 · see citing works
“The sensitivity of the present method is considerably more compared to previous reports where LLOQ was observed at 0.15 μg/mL and 0.1 μg/mL (Acharjya et al. 2012; Dhakne et al. 2020).”
MethodResultInfluential
- Authors
- Acharjya, S. K.
- Affiliations
- Roland Institute of Pharmaceutical Sciences (IN)
- Corresponding author
- Acharjya, Sasmita Kumari
- Venue
- Scientia Pharmaceutica
- Publisher
- MDPI AG
- Published
- 2012
- Volume
- 80
- Issue
- 4
- Pages
- 941-953
- ISSN
- 0036-8709 (ISSN-L)
- 2218-0532
- PMC
- PMC3528055
- OpenAlex
- W2020920654
- Cited by
- 23
- References
- 2
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 4 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 1 · 2013: 1
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Scientia Pharmaceutica (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Keywords
- letrozole, pharmacokinetics, serum drug quantification, reversed-phase HPLC, liquid-liquid extraction, diethyl ether extraction, Wistar rats, oral administration, bioanalytical method validation
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