Estimation of total flavonoid content in propolis by two complementary colometric methods
Open accessAbstract
Flavonoids, with various biological activities, are considered as key compounds in propolis. In this study, quantitative determinations of flavonoids in propolis were conducted by two complementary colorimetric methods, aluminum chloride method and 2,4-dinitrophenylhydrazine method. Results suggested that the sum of flavonoid contents determined by the above two individual methods may represent the real content of total flavonoids. In this work, six raw propolis samples were investigated and the total contents of flavonoids ranged from 10.38 ± 0.14% to 24.91 ± 0.53%. As for the 12 commercial propolis products examined, the levels of total flavonoids in tinctures were all below 7% and those in powdery products varied from 2.97 ± 0.05% to 22.73 ± 0.72%.
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- Authors
- Chang, C.-C., Yang, M.-H., Wen, H.-M., & Chern, J.-C.
- Venue
- Journal of Food and Drug Analysis
- Published
- 2020-07-14
- Volume
- 10
- Issue
- 3
- ISSN
- 1021-9498 (ISSN-L)
- 2224-6614
- OpenAlex
- W2261537439
- Cited by
- 4,106
- References
- 0
- Citations by year
- 2026: 245 · 2025: 303 · 2024: 308 · 2023: 301 · 2022: 271 · 2021: 389 · 2020: 308 · 2019: 293 · 2018: 226 · 2017: 198 · 2016: 209 · 2015: 210 · 2014: 223 · 2013: 198 · 2012: 151
- FWCI
- 222.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Food and Drug Analysis (publishedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
- Physical Sciences › Chemistry › Organic Chemistry › Plant-Derived Bioactive Compounds
- Health Sciences › Medicine › Pharmacology › Healthcare and Venom Research
- Keywords
- propolis, total flavonoid content, colorimetric determination, flavonoids
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