The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Comparison of isolation methods of exosomes and exosomal RNA from cell culture medium and serum
Open accessAbstract
Exosomes are cell-derived vesicles and are abundant in biological fluids; they contain RNA molecules which may serve as potential diagnostic biomarkers in 'precision medicine'. To promote the clinical application of exosomal RNA (exoRNA), many isolation methods must be compared and validated. Exosomes in cell culture medium (CCM) and serum may be isolated using ultracentrifugation (UC), ExoQuick or Total Exosome Isolation Reagent (TEI), and exoRNA may be extracted using TRIzol-LS, SeraMir, Total Exosome RNA Isolation (TER), HiPure Liquid RNA/miRNA kit (HLR), miRNeasy or exoRNeasy. ExoRNA was assessed using NanoDrop, Bioanalyzer 2100, quantitative polymerase chain reaction and high-throughput sequencing. UC showed the lowest recovery of particles, but the highest protein purity for exosome isolation. For isolation of exoRNA, we found that combinations of the TEI and TER methods resulted in high extraction efficiency and purity of small RNA obtained using CCM. High yield and a narrow size distribution pattern of small RNA were shown in exoRNA isolated by exoRNeasy from serum. In RNA profile analysis, the small RNA constituent ratio, miRNA content and amount varied as a result of methodological differences. This study showed that different methods may introduce variations in the concentration, purity and size of exosomes and exoRNA. Herein we discuss the advantages and disadvantages of each method and their application to different materials, therefore providing a reference according to research design.
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Cites
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Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
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Chen, J., & Chopp, M. (2018) · Stroke · doi:10.1161/strokeaha.117.018292 · Cited by 152
Geekiyanage, H., Rayatpisheh, S., Wohlschlegel, J. A., Brown, R., & Ambros, V. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2008323117 · Cited by 148
Picciolini, S., Gualerzi, A., Vanna, R., Sguassero, A., Gramatica, F., Bedoni, M., Masserini, M., & Morasso, C. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b00941 · Cited by 122
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Nie, C., Sun, Y., Zhen, H., Guo, M., Ye, J., Liu, Z., Yang, Y., & Zhang, X. (2020) · Frontiers in Neuroscience · doi:10.3389/fnins.2020.00438 · Cited by 89
Circulating exosomes in cardiovascular disease: Novel carriers of biological information
Liu, Q., Piao, H., Wang, Y., Zheng, D., & Wang, W. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.111148 · Cited by 70
Baek, A. E., Krawczynska, N., Das Gupta, A., Dvoretskiy, S. V., You, S., Park, J., Deng, Y.-H., Sorrells, J. E., Smith, B. P., Ma, L., et al. (2021) · Endocrinology · doi:10.1210/endocr/bqab095 · Cited by 43
Techniques for increasing the yield of stem cell-derived exosomes: What factors may be involved?
Feng, Z.-Y., Zhang, Q.-Y., Tan, J., & Xie, H.-Q. (2022) · Science China Life Sciences · doi:10.1007/s11427-021-1997-2 · Cited by 40
Genc, S., Pennisi, M., Yeni, Y., Yildirim, S., Gattuso, G., Altinoz, M. A., Taghizadehghalehjoughi, A., Bolat, I., Tsatsakis, A., Hacımüftüoğlu, A., et al. (2022) · Antioxidants · doi:10.3390/antiox11071225 · Cited by 33
Defining the Parameters for Sorting of RNA Cargo Into Extracellular Vesicles
Abdelgawad, A., Huang, Y., Gololobova, O., Yu, Y., Witwer, K. W., Parashar, V., & Batish, M. (2025) · Journal of Extracellular Vesicles · doi:10.1002/jev2.70113 · Cited by 26
Self-citationAljuhani, A., Albalawi, O., Albalawi, R., Alsalama, R., Alatawi, S., Altemani, R., Abdel‐Maksoud, M. S., & Elsherbiny, N. (2023) · Cell Biology International · doi:10.1002/cbin.12014 · Cited by 12
Metadata
- Authors
- Tang, Y.-T., Huang, Y.-Y., Zheng, L., Qin, S.-H., Xu, X.-P., An, T.-X., Xu, Y., Wu, Y.-S., Hu, X.-M., Ping, B.-H., & Wang, Q.
- Venue
- International Journal of Molecular Medicine
- Publisher
- Spandidos Publications
- Published
- 2017-07-24
- Volume
- 40
- Issue
- 3
- Pages
- 834-844
- ISSN
- 1107-3756 (ISSN-L)
- 1791-244X
- OpenAlex
- W2736407666
- Cited by
- 455
- References
- 3
- FWCI
- 14.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
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