Long lasting effect of acute restraint stress on behavior and brain anti-oxidative status
Open accessAbstract
Exposure to certain acute stressors results in an immediate behavioral and physiological response to these situations during a significant period of days. The goal of the current study is to evaluate the long-lasting effect of single exposure of restraint stress among mice after 0 h, 24 h, 48 h and 72 h. Five groups of mice are under experiment: a control group and four groups exposed to one session of restraint stress. All these groups have been studied for behavioral tests in order to evaluate their memories. This is done through a Y-labyrinth and an object recognition test, and anxiety by using open field device. In the second part of the study, enzymatic assays (concerning catalase, glutathione s transferase, glutathione peroxidase and superoxide dismutase) are used to evaluate oxidative stress. The enzymatic activity of the antioxidant system is assessed in five brain structures, including the cerebellum, olfactory bulb, spinal bulb, hypothalamus, and hippocampus. The obtained results show that acute restraint stress leads to a decrease in memory function and to the development of an anxious state; concomitant to an increase of locomotor activity afterword. It causes disturbance of antioxidant balance in the brain by developing a state of oxidative stress. Indeed, restraint stress causes a change in anti-oxidant stress enzymatic activity in the brain, notably in post-stress period. In conclusion, acute restraint stress is responsible for altering cognitive functions, especially memory, and the development of anxious behavior, which could be a result of the generation of oxidative stress; effects that are persistent over an important period after the cessation of stress.
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Restraint stress in rats leads to a decrease in memory function, the development of anxiety-like functions, and an increase in motor activity, changing the functional state of antioxidant enzymes in the brain, especially in the poststress period.
“Restraint stress in rats leads to a decrease in memory function, the development of anxiety-like functions, and an increase in motor activity, changing the functional state of antioxidant enzymes in the brain, especially in the poststress period [33,34].”
From Anxiolytic and Antioxidant Effect of Phytoecdysteroids and Polyphenols from Chenopodium quinoa on an In Vivo Restraint Stress Model (2022) · cites this paper as [34]
Under immobilization stress, there is a significant increase in acetylcholinesterase activity in some parts of the rat brain.
“Under immobilization stress, there is a significant increase in acetylcholinesterase activity in some parts of the rat brain [34].”
From Anxiolytic and Antioxidant Effect of Phytoecdysteroids and Polyphenols from Chenopodium quinoa on an In Vivo Restraint Stress Model (2022) · cites this paper as [34]
The nutritional support of FFIs contributed to hypolipidemic action in rats, some compensation of the circulating transaminases activity, and normalization to control values of malondialdehyde levels.
“The nutritional support of FFIs contributed to hypolipidemic action in rats, some compensation of the circulating transaminases activity, and normalization to control values of malondialdehyde levels, the concentration of which increases in all brain structures during immobilization stress, reflecting the rate of the formation of reactive oxygen species [34].”
From Anxiolytic and Antioxidant Effect of Phytoecdysteroids and Polyphenols from Chenopodium quinoa on an In Vivo Restraint Stress Model (2022) · cites this paper as [34]
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O. H., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 315,771 · see citing works
A new and rapid colorimetric determination of acetylcholinesterase activity
Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961) · Biochemical Pharmacology · doi:10.1016/0006-2952(61)90145-9 · Cited by 27,221 · see citing works
Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42083-8 · Cited by 18,396 · see citing works
[30] Microsomal lipid peroxidation
Buege, J. A., & Aust, S. D. (1978) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(78)52032-6 · Cited by 13,437 · see citing works
[12] Assays of glutathione peroxidase
Flohé, L., & Günzler, W. A. (1984) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(84)05015-1 · Cited by 4,954 · see citing works
Aebi, H. E. (1974) · Elsevier eBooks · doi:10.1016/b978-0-12-091302-2.50032-3 · Cited by 2,075 · see citing works
Stress, eating and the reward system
Adam, T. C., & Epel, E. S. (2007) · Physiology & Behavior · doi:10.1016/j.physbeh.2007.04.011 · Cited by 1,732 · see citing works
The role of acetylcholine in learning and memory
Hasselmo, M. E. (2006) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2006.09.002 · Cited by 1,693 · see citing works
Acetylcholine as a Neuromodulator: Cholinergic Signaling Shapes Nervous System Function and Behavior
Picciotto, M. R., Higley, M. J., & Mineur, Y. S. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.036 · Cited by 1,404 · see citing works
Pesticides and oxidative stress: a review.
Abdollahi, M. R., Ranjbar, A., Shadnia, S., Nikfar, S., & Rezaie, A. R. (2004) · PubMed · Cited by 1,010 · see citing works
Chaudhuri, O., Koshy, S. T., Branco da Cunha, C., Shin, J.-W., Verbeke, C. S., Allison, K. H., & Mooney, D. J. (2014) · Nature Materials · doi:10.1038/nmat4009 · Cited by 820 · see citing works
A Molecular Web: Endoplasmic Reticulum Stress, Inflammation, and Oxidative Stress
Chaudhari, N., Talwar, P., Parimisetty, A., Lefebvre d’Hellencourt, C., & Ravanan, P. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00213 · Cited by 600 · see citing works
Santos, C. X. C., Tanaka, L. Y., Wosniak, J., & Laurindo, F. R. M. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2009.2625 · Cited by 572 · see citing works
Cognitive factors in odor detection, odor discrimination, and odor identification tasks
Hedner, M., Larsson, M. U., Arnold, N., Zucco, G. M., & Hummel, T. R. (2010) · Journal of Clinical and Experimental Neuropsychology · doi:10.1080/13803391003683070 · Cited by 472 · see citing works
Cholinergic modulation of the hippocampal region and memory function
Haam, J., & Yakel, J. L. (2017) · Journal of Neurochemistry · doi:10.1111/jnc.14052 · Cited by 446 · see citing works
THE MUTANT mdx : INHERITED MYOPATHY IN THE MOUSE
TORRES, L. F. B., & Duchen, L. W. (1987) · Brain · doi:10.1093/brain/110.2.269 · Cited by 352 · see citing works
Akhgari, M., Abdollahi, M. R., Kebryaeezadeh, A., Hosseini, R., & Sabzevari, O. (2003) · Human & Experimental Toxicology · doi:10.1191/0960327103ht346oa · Cited by 329 · see citing works
Chaplin, T., Hong, K., Bergquist, K. L., & Sinha, R. (2008) · Alcoholism: Clinical and Experimental Research · doi:10.1111/j.1530-0277.2008.00679.x · Cited by 309 · see citing works
Stress and Diabetes: A Review of the Links
Lloyd, C., Smith, J., & Weinger, K. (2005) · Diabetes Spectrum · doi:10.2337/diaspect.18.2.121 · Cited by 248 · see citing works
Assay and Inhibitors of Spinach Superoxide Dismutase
Asada, K., Takahashi, M., & NAGATE, M. (1974) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1974.10861178 · Cited by 248 · see citing works
Object recognition testing: Methodological considerations on exploration and discrimination measures
Akkerman, S., Blokland, A., Reneerkens, O. A. H., van Goethem, N. P., Bollen, E., Gijselaers, H. J. M., Lieben, C. K. J., Steinbusch, H. W. M., & Prickaerts, J. (2012) · Behavioural Brain Research · doi:10.1016/j.bbr.2012.03.022 · Cited by 208 · see citing works
Septohippocampal Acetylcholine: Involved in but not Necessary for Learning and Memory?Preprint
Parent, M. B., & Baxter, M. G. (2004) · Learning & Memory · doi:10.1101/lm.69104 · Cited by 194 · see citing works
Cholinergic modulation of cognitive processing: insights drawn from computational models
Newman, E. L., Gupta, K., Climer, J. R., Monaghan, C. K., & Hasselmo, M. E. (2012) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2012.00024 · Cited by 185 · see citing works
Acetylcholine and memory: A long, complex and chaotic but still living relationship
Micheau, J., & Marighetto, A. (2010) · Behavioural Brain Research · doi:10.1016/j.bbr.2010.11.052 · Cited by 164 · see citing works
Psychological stress, immune response, and atherosclerosis
Gu, H., Tang, C., & Yang, Y.-Z. (2012) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2012.01.021 · Cited by 156 · see citing works
Malondialdehyde as a marker of oxidative stress in periodontitis patients
Cherian, D., Peter, T., Narayanan, A., Madhavan, S., Achammada, S., & Vynat, G. P. (2019) · Journal of Pharmacy And Bioallied Sciences · doi:10.4103/jpbs.jpbs_17_19 · Cited by 156 · see citing works
Espèces réactives de l’oxygène et stress oxydant
Migdal, C., & Serres, M. (2011) · médecine/sciences · doi:10.1051/medsci/2011274017 · Cited by 146 · see citing works
Single exposure to stressors causes long-lasting, stress-dependent reduction of food intake in rats
Vallès, A., Martı́, O., García, A., & Armario, A. (2000) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2000.279.3.r1138 · Cited by 145 · see citing works
Harris, R. B. S., Mitchell, T. D., Simpson, J., Redmann, S. M., Youngblood, B. D., & Ryan, D. H. (2002) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.2002.282.1.r77 · Cited by 139 · see citing works
ERAD and ERAD tuning: disposal of cargo and of ERAD regulators from the mammalian ER
Bernasconi, R., & Molinari, M. (2010) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2010.10.002 · Cited by 137 · see citing works
Neuroendocrine Regulation of Brain Cytokines After Psychological Stress
Johnson, J. D., Barnard, D. F., Kulp, A. C., & Mehta, D. M. (2019) · Journal of the Endocrine Society · doi:10.1210/js.2019-00053 · Cited by 135 · see citing works
Paskitti, M., McCreary, B. J., & Herman, J. P. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00121-2 · Cited by 116 · see citing works
Bland, S. T., Schmid, M. J., Der-Avakian, A., Watkins, L., Spencer, R. L., & Maier, S. (2005) · Brain Research · doi:10.1016/j.brainres.2005.05.065 · Cited by 115 · see citing works
Toxic effects of diazinon and its photodegradation products
Čolović, M. B., Krstic, D. Z., Petrović, S. Ž., Leskovac, A. R., Joksiċ, G., Savić, J., Franko, M., Trebše, P., & Vasić, V. (2009) · Toxicology Letters · doi:10.1016/j.toxlet.2009.11.022 · Cited by 106 · see citing works
Hayashi, T. (2015) · Psychiatry and Clinical Neurosciences · doi:10.1111/pcn.12262 · Cited by 87 · see citing works
Quick, S. K., Shields, P. G., Nie, J., Platek, M. E., McCann, S. E., Hutson, A. D., Trevisan, M., Vito, D., Modali, R. V., Lehman, T. A., et al. (2008) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-07-2755 · Cited by 54 · see citing works
Inflammation as an Intermediate Pathway in the Association between Psychosocial Stress and Obesity
Hamer, M., & Stamatakis, E. (2008) · Physiology & Behavior · doi:10.1016/j.physbeh.2008.03.010 · Cited by 52 · see citing works
Coagulation Activity Before and After Acute Psychosocial Stress Increases With Age
Wirtz, P. H., Redwine, L. S., Bärtschi, C., Dickson‐Spillmann, M., Ehlert, U., & von Känel, R. (2008) · Psychosomatic Medicine · doi:10.1097/psy.0b013e31816e03a5 · Cited by 36 · see citing works
Kovács, L. Á., Schiessl, J. A., Nafz, A. E., CSERNUS, V. J., & Gaszner, B. (2018) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2018.00248 · Cited by 35 · see citing works
Manni, L., Fausto, V. D., Fiore, M., & Aloe, L. (2008) · Current Neurovascular Research · doi:10.2174/156720208783565654 · Cited by 30 · see citing works
Dal-Zotto, S., Mart�, O., Delgado‐Morales, R., & Armario, A. (2004) · Psychopharmacology · doi:10.1007/s00213-004-1939-y · Cited by 23 · see citing works
Chigr, F., Rachidi, F., Tardivel, C., Najimi, M., & Moyse, E. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00198 · Cited by 21 · see citing works
Deschamps, R., & Moulignier, A. (2005) · Encyclopédie médico-chirurgicale. Neurologie · doi:10.1016/j.emcn.2005.07.003 · Cited by 16 · see citing works
BDNF regulation in the rat dorsal vagal complex during stress-induced anorexia
Charrier, C., Chigr, F., Tardivel, C., Mahaut, S., Jean, A., Najimi, M., & Moyse, E. (2006) · Brain Research · doi:10.1016/j.brainres.2006.05.099 · Cited by 14 · see citing works
Laurent, L., Jean, A., Manrique, C., Najimi, M., Chigr, F., & Compan, V. (2012) · Neuromethods · doi:10.1007/978-1-62703-104-2_19 · Cited by 5 · see citing works
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Rai, A. R., Joy, T., Poojari, M. S., Pai, M. M., Massand, A., & Murlimanju, B. V. (2023) · Veterinary World · doi:10.14202/vetworld.2023.1755-1764 · Cited by 14 · see citing works
Jeon, D., Kim, S., Kim, J., Jeong, H., Uhm, C., Oh, H., Cho, K., Cho, Y., Park, I. H., Oh, J., et al. (2024) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2024.167347 · Cited by 12 · see citing works
Sidorova, Y. S., Shipelin, V. A., Petrov, N. A., Zorin, S. N., & Mazo, V. K. (2022) · Molecules · doi:10.3390/molecules27249003 · Cited by 11 · see citing works
Li, C., Munalisa, R., Lee, H.-Y., Lien, T.-S., Chan, H., Hung, S., Sun, D., Cheng, C., & Chang, H. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241612877 · Cited by 9 · see citing works
Lanshakov, D. A., Sukhareva, E. V., Bulygina, V. V., Khozyainova, A. A., Gerashchenko, T. S., Denisov, E. V., & Kalinina, T. S. (2024) · Scientific Reports · doi:10.1038/s41598-024-73042-2 · Cited by 6 · see citing works
Acute Restraint Stress Enhances Prosocial Behavior in Rats via Oxytocin and Fear-Related Circuits
Wang, S.-C., Lin, C.-C., Chen, C. C., & Liu, Y. (2025) · Journal of Integrative Neuroscience · doi:10.31083/jin33400 · Cited by 5 · see citing works
Balatskyi, V. A., Gospodaryov, D. V., Abrat, O., Strilbytska, O. M., Demianchuk, O., Tkachyk, A., Vasylyshyn, O., Lushchak, O. V., Lushchak, V. I., & Bayliak, M. M. (2025) · Physiology & Behavior · doi:10.1016/j.physbeh.2025.115065 · Cited by 6 · see citing works
Kurnianingsih, N., Artamevia, D., Winarta, A., Wulandari, A., Siregar, S., Hasanah, D., Widodo, E., & Ratmawati, R. (2023) · Journal of Tropical Life Science · doi:10.11594/jtls.13.02.18 · Cited by 4 · see citing works
Engborg, C. B., Pujar, M., Kanadia, R., & Sciolino, N. R. (2025) · Neuroscience · doi:10.1016/j.neuroscience.2025.08.046 · Cited by 2 · see citing works
Matos, J. H. B., Silva, A. H. B. D. L., Ferreira, M. V., Verri, W. A., Jr., da Cunha, J. M., & Zanoveli, J. M. (2025) · Brain Research · doi:10.1016/j.brainres.2025.149612 · Cited by 1 · see citing works
Dalui, S., Pal, S., Goswami, A., Sinha, A., Karar, S., Saha, A., Modak, P., Sil, M., Ratnaparkhi, P., Goswami, A., et al. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05434-y · Cited by 1 · see citing works
Vijay, V., Ray, S., Kingsly, R., Sharma, P., Kumar, A., & Chakravarty, S. (2026) · Physiology & Behavior · doi:10.1016/j.physbeh.2026.115288 · Cited by 1 · see citing works
Lee, D., Jeong, H., Gwon, H., Lee, K., Kim, J.-Y., Shim, J.-J., & Lee, J.-H. (2026) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.2604.04063 · Cited by 1 · see citing works
Mizuhara, R., Amemiya, S., Maruyama, T., & Kita, I. (2025) · Physiology & Behavior · doi:10.1016/j.physbeh.2025.115081 · Cited by 1 · see citing works
Vijay, V., Ray, S., Kingsly, R., Sharma, P., Kumar, A., & Chakravarty, S. (2025) · Research Square · doi:10.21203/rs.3.rs-8056455/v1
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Capellano, L. (2025) · theses.fr (ABES) · doi:10.70675/dd0b8f65z490fz446fz8d1azd0dd5619a044
- Authors
- Chaoui, N., Biological Engineering Laboratory, F. O. S. A. T., Anarghou, H., Laaroussi, M., Essaidi, O., Najimi, M., & Chigr, F.
- Affiliations
- Université Sultan Moulay Slimane (MA)
- Corresponding author
- Chigr, Fatiha
- Venue
- AIMS neuroscience
- Published
- 2022
- Volume
- 9
- Issue
- 1
- Pages
- 57-75
- ISSN
- 2373-7972 (ISSN-L)
- PMC
- PMC8941193
- OpenAlex
- W4210415950
- Cited by
- 16
- References
- 40
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 2 · 2023: 3 · 2022: 1
- FWCI
- 2.07
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- AIMS neuroscience (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- acute restraint stress, memory impairment, anxiety, locomotor activity, oxidative stress, antioxidant enzymes, hippocampus, cerebellum, olfactory bulb, hypothalamus, spinal cord, Y-maze, object recognition, mice
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