Characterization of murine lung interstitial macrophages in comparison with alveolar macrophages in vitro

Franke-Ullmann, G; Pförtner, C; Walter, P; Steinmüller, C; Lohmann-Matthes, M L; Kobzik, L

doi:10.4049/jimmunol.157.7.3097

Cited by 160Phagocytosis and Immune RegulationImmune cells in cancerImmune Response and Inflammation

Characterization of murine lung interstitial macrophages in comparison with alveolar macrophages in vitro

Franke-Ullmann, G, Pförtner, C, Walter, P, Steinmüller, C, Lohmann-Matthes, M L, Kobzik, L·1996·The Journal of Immunology

DOI 10.4049/jimmunol.157.7.3097

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Abstract

The present study was performed to characterize the immunologic potential of interstitial macrophages (INT) in comparison with alveolar macrophages (AL). The data showed that AL, compared with INT, have a more efficient phagocytic potential. In addition, they have a strong microbicidal activity and secrete large amounts of reactive oxygen radicals, nitric oxides, TNF, and IFN on appropriate stimulation. They also exert strong tumoricidal and parasiticidal activities. In contrast, INT are more efficient in releasing immunoregulatory cytokines such as IL-1 and IL-6. As determined by Ab staining, INT express more MHC class II molecules and are more effective in functioning as accessory cells for mitogen-stimulated lymphocyte proliferation compared with AL. Thus, AL appear to be particularly effective as nonspecific first line defense cells against infectious agents, whereas INT are equipped to cooperate with interstitial lymphocytes in inducing a specific immune reaction.

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Resident alveolar macrophages have high phagocytic capacity.
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From Depletion of alveolar macrophages during influenza infection facilitates bacterial superinfections (2013) · cites this paper as [14]

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“Some evidence suggests, however, that they may in fact act like other macrophages as professional antigen-presenting cells.(18,33) Interstitial DCs are found throughout the lungs and upper respiratory tract.”
Background
Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections
Andrade, C. A., Pacheco, G. A., Gálvez, N. M. S., Soto, J. A., Bueno, S. M., & Kalergis, A. M. (2020) · Viruses · doi:10.3390/v12060637 · Cited by 42 · see citing works
“Macrophages are within the first cells that protect the host against the viral infection, and there are two types in the lungs: the alveolar macrophages (AMs) and the interstitial macrophages (IMs) [138].”
Background
Myeloid hypoxia-inducible factor 1α prevents airway allergy in mice through macrophage-mediated immunoregulation
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MiR-27-3pregulates TLR2/4-dependent mouse alveolar macrophage activation by targetting PPARγ
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The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease
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“The predominance and contribution of different macrophage subpopulations to the immunopathogenesis of COPD is unclear, especially given the complexity and diversity that entails themacrophage population [34,35].”
Background
Glucocorticoid-Augmented Efferocytosis Inhibits Pulmonary Pneumococcal Clearance in Mice by Reducing Alveolar Macrophage Bactericidal Function
Stolberg, V. R., McCubbrey, A. L., Freeman, C. M., Brown, J. P., Crudgington, S., Taitano, S. H., Saxton, B. L., Mancuso, P., & Curtis, J. L. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1402217 · Cited by 39 · see citing works
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Yousaf, H., Khan, M. I. U., Ali, I., Munir, M. U., & Lee, K. Y. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.114426 · Cited by 35 · see citing works
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“This heterogeneity results in a population of cells with variable immune function (15).”
Background
Oxygen Tension Modulates Differentiation and Primary Macrophage Functions in the Human Monocytic THP-1 Cell Line
Grodzki, A. C., Giulivi, C., & Lein, P. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054926 · Cited by 34 · see citing works
“In contrast, there are numerous reports that alveolar macrophages have greater functional activity related to antimicrobial defense, including phagocytosis, compared to interstitial macrophages which display enhanced capabilities relevant to specific immune responses such as antigen processing as well as in antioxidan…”
Background
25‐Hydroxycholesterol metabolism is altered by lung inflammation, and its local administration modulates lung inflammation in mice
Bottemanne, P., Paquot, A., Ameraoui, H., et al. (2021) · The FASEB Journal · doi:10.1096/fj.202002555r · Cited by 31 · see citing works
Clinical Management of Complicated Urinary Tract Infection
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The Compromise of Macrophage Functions by Hyperoxia Is Attenuated by Ethacrynic Acid via Inhibition of NF-κB–Mediated Release of High-Mobility Group Box-1
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“reactive oxygen intermediates, nitric oxide (NO), TNF-a and interferons following stimulation.(71) Macrophages also contribute to immune responses by releasing IL-1 and IL-6, and by upregulating MHC molecules to more efficiently present antigen to recruited lymphocytes.”
Background
Plasticity towards Rigidity: A Macrophage Conundrum in Pulmonary Fibrosis
Sari, E., He, C., & Margaroli, C. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231911443 · Cited by 26 · see citing works
Macrophages are related to goblet cell hyperplasia and induce MUC5B but not MUC5AC in human bronchus epithelial cells
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Type I Interferons Are Involved in the Intracellular Growth Control of Mycobacterium abscessus by Mediating NOD2-Induced Production of Nitric Oxide in Macrophages
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“(D) AMs were incubated with MAB (MOI 1:25) and indicated reagents (2 h pretreated, L-NAME 1 mM, rIFN-b 1,000 units/ml).”
BackgroundInfluential
Mitochondrial Regulation of Inflammasome Activation in Chronic Obstructive Pulmonary Disease
Yoon, C. M., Nam, M., Oh, Y., Cruz, C. S. D., & Kang, M. (2015) · Journal of Innate Immunity · doi:10.1159/000441299 · Cited by 23 · see citing works
Evaluation of cytokine gene expression in porcine spleen cells, peripheral blood mononuclear cells, and alveolar macrophages by competitive RT-PCR
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“Pig and mouse alveolar macrophages are known to have potent phagocytic abilities and to be good sources of cytokine production, including IL-1, TNF-K, and IFN-Q [5,26,27].”
Background
Altered expression and localization of 5-lipoxygenase accompany macrophage differentiation in the lung
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Background
Sequential targeting of interferon pathways for increased host resistance to bacterial superinfection during influenza
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Heterogeneity of Lung Mononuclear Phagocytes in Chronic Obstructive Pulmonary Disease
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Method
GTS-21, an α7nAChR agonist, increases pulmonary bacterial clearance in mice by restoring hyperoxia-compromised macrophage function
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Background
Lineage tracing reveals the origins and dynamics of macrophages in lung injury and repair
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Authors: Franke-Ullmann, G., Pförtner, C., Walter, P., Steinmüller, C., Lohmann-Matthes, M. L., & Kobzik, L.
Affiliations: Fraunhofer Institute for Toxicology and Experimental Medicine (DE)
ORCID: Kobzik, L
Venue: The Journal of Immunology
Publisher: Oxford University Press (OUP)
Published: 1996-10-01
Volume: 157
Issue: 7
Pages: 3097-3104
ISSN: 0022-1767 (ISSN-L)
1550-6606
DOI: 10.4049/jimmunol.157.7.3097
OpenAlex: W1539936851
Cited by: 160
References: 0
Citations by year: 2026: 3 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 7 · 2021: 12 · 2020: 8 · 2019: 7 · 2018: 7 · 2017: 4 · 2016: 8 · 2015: 10 · 2014: 7 · 2013: 12 · 2012: 5
FWCI: 0.69
Type: journal article
Language: en
Open access: closed
Licence: academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations: The Journal of Immunology (publishedVersion)
PubMed (publishedVersion)
Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
Topics: Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords: interstitial macrophages, alveolar macrophages, lung macrophages, phagocytosis, microbicidal activity, reactive oxygen radicals, nitric oxide, TNF-α, interferon, immunoregulatory cytokines, MHC class II, accessory cells, lymphocyte proliferation, innate immunity, adaptive immunity, mouse lung
MeSH terms: Interleukin-6 Inhibitors, Animals, Antigen-Presenting Cells, Cells, Cultured, Female, Interferons, Interleukin-1, L Cells, Leishmania donovani, Lung, Macrophage Activation, Macrophages, Mice, Inbred BALB C, Phagocytosis, Mast-Cell Sarcoma, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha, Interleukin-6, Macrophages, Alveolar, Reactive Oxygen Species, Mice

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Characterization of murine lung interstitial macrophages in comparison with alveolar macrophages in vitro

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