CoChat
Sign Up
Cited by 564Inflammasome and immune disordersAdenosine and Purinergic SignalingImmune Response and Inflammation

The DOI matches its Crossref record; 74 of the 75 references it cites were found in a registry or an index.

Nonclassical IL-1β Secretion Stimulated by P2X7 Receptors Is Dependent on Inflammasome Activation and Correlated with Exosome Release in Murine Macrophages

Qu, Yan, Franchi, Luigi, Nunez, Gabriel, Dubyak, George R·2007·The Journal of Immunology
DOI 10.4049/jimmunol.179.3.1913
Open this paper in CoChat — free account

Abstract

Several mechanistically distinct models of nonclassical secretion, including exocytosis of secretory lysosomes, shedding of plasma membrane microvesicles, and direct efflux through plasma membrane transporters, have been proposed to explain the rapid export of caspase-1-processed IL-1β from monocytes/macrophages in response to activation of P2X7 receptors (P2X7R) by extracellular ATP. We compared the contribution of these mechanisms to P2X7R-stimulated IL-1β secretion in primary bone marrow-derived macrophages isolated from wild-type, P2X7R knockout, or apoptosis-associated speck-like protein containing a C-terminal CARD knockout mice. Our experiments revealed the following: 1) a novel correlation between IL-1β secretion and the release of the MHC-II membrane protein, which is a marker of plasma membranes, recycling endosomes, multivesicular bodies, and released exosomes; 2) a common and absolute requirement for inflammasome assembly and active caspase-1 in triggering the cotemporal export of IL-1β and MHC-II; and 3) mechanistic dissociation of IL-1β export from either secretory lysosome exocytosis or plasma membrane microvesicle shedding on the basis of different requirements for extracellular Ca2+ and differential sensitivity to pharmacological agents that block activation of caspase-1 inflammasomes. Thus, neither secretory lysosome exocytosis nor microvesicle shedding models constitute the major pathways for nonclassical IL-1β secretion from ATP-stimulated murine macrophages. Our findings suggest an alternative model of IL-1β release that may involve the P2X7R-induced formation of multivesicular bodies that contain exosomes with entrapped IL-1β, caspase-1, and other inflammasome components.

Overview

Integrity signals

Registry

Crossref · 10.4049/jimmunol.179.3.1913

Cites

74 of 74 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 75 references

1 of the 75 references couldn’t be matched to a known paper.

Research works printed but not linked: 73

  1. 1996, Blood, 2095
  2. 1993, J. Biol. Chem., 18062
  3. 1995, Cell, 401
  4. 2002, Mol. Cell, 417
  5. 2006, Cell, 659
  6. 2002, J. Biol. Chem., 21119
  7. 2003, Nat. Rev. Mol. Cell Biol., 95
  8. 2005, Annu. Rev. Biochem., 355
  9. 2001, J. Biol. Chem., 28309
  10. 2004, Nature, 213
  11. 2006, Nature, 228
  12. 2006, J. Biol. Chem., 35217
  13. 2006, Nat. Immunol., 576
  14. 2006, Nat. Immunol., 569
  15. 2006, Nature, 233
  16. 2006, J. Biol. Chem., 36560
  17. 2006, Nature, 237
  18. 2006, Immunity, 317
  19. 1994, J. Biol. Chem., 15195
  20. 1997, J. Immunol., 1451
  21. 1999, J. Biol. Chem., 36944
  22. 2001, J. Biol. Chem., 125
  23. 1992, J. Immunol., 1294
  24. 1995, EMBO J., 1607
  25. 1998, Am. J. Physiol., C1538
  26. 2004, Mol. Pharmacol., 909
  27. 2004, Proc. Natl. Acad. Sci. USA, 10241
  28. 1990, EMBO J., 1503
  29. 1991, Biochem. Soc. Trans., 255
  30. 2005, J. Immunol., 7611
  31. 2002, J. Biol. Chem., 3210
  32. 2005, J. Immunol., 7623
  33. 1999, Mol. Biol. Cell, 1463
  34. 2004, Proc. Natl. Acad. Sci. USA, 9745
  35. 2006, Blood, 1618
  36. 2001, Immunity, 825
  37. 2005, J. Immunol., 7268
  38. 2007, Blood, 3856
  39. 2007, J. Cell Sci., 772
  40. 2005, Methods Mol. Biol., 91
  41. 2003, Am. J. Physiol., C286
  42. 1999, Eur. J. Biochem., 100
  43. 2003, J. Immunol., 5728
  44. 2000, Trends Cell Biol., 316
  45. 1997, J. Cell Biol., 93
  46. 2001, Cell, 157
  47. 2006, J. Biol. Chem., 35147
  48. 2006, Br. J. Pharmacol., 324
  49. 2002, J. Immunol., 6436
  50. 2003, J. Immunol., 3029
  51. 2004, Am. J. Physiol., C1100
  52. 1996, J. Biol. Chem., 13273
  53. 2006, EMBO J., 5071
  54. 2007, J. Biol. Chem., 2386
  55. 2000, J. Immunol., 5120
  56. 2002, Nat. Rev. Immunol., 569
  57. 2004, Curr. Opin. Cell Biol., 415
  58. 1997, Blood, 2911
  59. 2005, Pain, 386
  60. 2005, Pain, 37
  61. 2004, Pain, 229
  62. 2002, Nat. Rev. Mol. Cell Biol., 122
  63. 2005, Blood Cells Mol. Dis., 94
  64. 2006, J. Biochem., 13
  65. 2006, Cell, 1135
  66. 2005, J. Biol. Chem., 33968
  67. 2002, J. Biol. Chem., 34017
  68. 2004, J. Leukocyte Biol., 1173
  69. 2007, J. Biol. Chem., 3403
  70. 1997, J. Cell Biol., 697
  71. 2006, J. Immunol., 7257
  72. 2000, J. Immunol., 379
  73. 2007, Immunity, 433

Cited by

Sorted by citations

See all 564

Related

Metadata

Authors
Qu, Y., Franchi, L., Nunez, G., & Dubyak, G. R.
Affiliations
  • Case Western Reserve University (US)
  • University of Michigan (US)
  • Department of Pathology, University of Michigan , Ann Arbor, MI 48109
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2007-08-01
Volume
179
Issue
3
Pages
1913-1925
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.179.3.1913
OpenAlex
W2100358971
Cited by
563
References
75
Citations by year
2026: 16 · 2025: 21 · 2024: 21 · 2023: 26 · 2022: 24 · 2021: 27 · 2020: 36 · 2019: 28 · 2018: 36 · 2017: 30 · 2016: 30 · 2015: 43 · 2014: 37 · 2013: 36 · 2012: 38
FWCI
9.93
Type
journal article
Language
en
Open access
closed
Licence
academic.oup.com/pages/standard-publication-reuse-rights (vor)
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of General Medical Sciences (GM-36387, R01GM036387)
  • National Institute of Allergy and Infectious Diseases (AI 064748, AI063331, R01AI064748)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
P2X7 receptor, inflammasome, caspase-1, exosomes, macrophages, extracellular ATP, multivesicular bodies, extracellular calcium
MeSH terms
Adenosine Triphosphate, Animals, Cathepsin B, Cells, Cultured, Enzyme Activation, Exocytosis, Inflammation, Lysosomes, Macrophages, Mice, Inbred BALB C, Mice, Inbred C57BL, Endosomes, Signal Transduction, Receptors, Purinergic P2, Mice, Knockout, Models, Immunological, Caspase 1, Multiprotein Complexes, Mice, Interleukin-1beta, Receptors, Purinergic P2X7

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review