Autophagy-dependent ATP release from dying cells via lysosomal exocytosis
Open accessAbstract
ATP not only represents a key molecule for the intracellular storage and utilization of energy, but also plays a central role in the extracellular milieu, as it both recruits and activates immune cells. The secretion of ATP in the course of immunogenic cell death (ICD), a peculiar type of apoptosis that elicits adaptive immune responses, critically relies on the molecular machinery for autophagy. We have recently demonstrated that autophagy allows for the ICD-associated secretion of ATP as it contributes to the maintenance of lysosomal ATP stores. In addition, we found that ATP release in this setting is mediated by a molecular mechanism that involves lysosomal-associated membrane protein 1 (LAMP1)- and pannexin 1 (PANX1)-dependent lysosomal exocytosis.
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In ICD, ATP release occurs downstream of caspase activation and involves autolysosomal ATP accumulation, LAMP1 relocalization, ROCK1-dependent blebbing, and PANX1 opening.
“In the context of ICD, ATP is released through a cascade of events occurring downstream of caspase activation and involving: (1) the autophagy-dependent accumulation of ATP within autolysosomes (the organelles forming by the fusion of autophagosomes or amphisomes with lysosomes), (2) the relocalization of lysosomal-associated membrane protein 1 (LAMP1) to the plasma membrane, (3) ROCK1-dependent”
From Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. (2018) · cites this paper as [898]
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Self-citation
- Authors
- Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G.
- Affiliations
- Université Paris-Sud (FR)
- Institut Gustave Roussy (FR)
- Délégation Paris 5 (FR)
- Corresponding author
- Kroemer, Guido
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2013-10-25
- Volume
- 9
- Issue
- 10
- Pages
- 1624-1625
- ISSN
- 1554-8627 (ISSN-L)
- 1554-8635
- OpenAlex
- W2093037778
- Cited by
- 137
- References
- 0
- Citations by year
- 2026: 9 · 2025: 9 · 2024: 9 · 2023: 14 · 2022: 17 · 2021: 12 · 2020: 10 · 2019: 9 · 2018: 15 · 2017: 10 · 2016: 10 · 2015: 5 · 2014: 7 · 2013: 1
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Autophagy (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- ATP release, immunogenic cell death, lysosomal exocytosis, LAMP1, pannexin 1, dying cells, extracellular ATP, adaptive immunity
- MeSH terms
- Adenosine Triphosphate, Animals, Autophagy, Exocytosis, Humans, Nerve Tissue Proteins, Apoptosis, Lysosomal-Associated Membrane Protein 1
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