The DOI matches its Crossref record.
Physical and biological aspects of renal vitrification
Abstract
Cryopreservation would potentially very much facilitate the inventory control and distribution of laboratory-produced organs and tissues. Although simple freezing methods are effective for many simple tissues, bioartificial organs and complex tissue constructs may be unacceptably altered by ice formation and dissolution. Vitrification, in which the liquids in a living system are converted into the glassy state at low temperatures, provides a potential alternative to freezing that can in principle avoid ice formation altogether. The present report provides a brief overview of the problem of renal vitrification. We report here the detailed case history of a rabbit kidney that survived vitrification and subsequent transplantation, a case that demonstrates both the fundamental feasibility of complex system vitrification and the obstacles that must still be overcome, of which the chief one in the case of the kidney is adequate distribution of cryoprotectant to the renal medulla. Medullary equilibration can be monitored by monitoring urine concentrations of cryoprotectant, and urine flow rate correlates with vitrification solution viscosity and the speed of equilibration. By taking these factors into account and by using higher perfusion pressures as per the case of the kidney that survived vitrification, it is becoming possible to design protocols for equilibrating kidneys that protect against both devitrification and excessive cryoprotectant toxicity.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Fahy et al.'s transplant sustained the life of the recipient but with significantly impaired renal function and the need for repeated blood transfusion.
“While their transplant did sustain the life of the recipient, it did so with significantly impaired renal function and the need for repeated blood transfusion.”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [31]
During CPA perfusion loading of organs, successfully perfused tissues may only equilibrate to ~92-94% concentration of full-strength CPA.
“during CPA perfusion loading of organs, successfully perfused tissues may only equilibrate to ~92-94% concentration of full-strength CPA15,34”
From Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation (2025) · cites this paper as [34]
Fahy et al.'s transplant recipient had a nadir creatinine of ~4 mg/dL and a terminal creatinine of ~6 mg/dL.
“Further, their transplant recipient had a nadir creatinine of ~4 mg/dL and a terminal creatinine of ~6 mg/dL”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [31]
Fahy et al.'s single surviving rabbit kidney transplant was vitrified for only 8 min prior to rewarming.
“Additionally, their single surviving rabbit kidney transplant was vitrified for only 8 min prior to rewarming”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [31]
Fahy and colleagues reported a single successful rabbit kidney transplant.
“and reported a single successful rabbit kidney transplant31”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [31]
Inadequate tissue CPA concentration combined with suboptimal rewarming led to ice formation, particularly in the renal medulla, and failure in almost all transplants.
“Inadequate tissue CPA concentration, combined with suboptimal rewarming, led to ice formation, particularly in the renal medulla, and failure in almost all transplants21,31.”
From Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model (2023) · cites this paper as [31]
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 56
- Fahy GM, 2006, Rejuvenation Res, 9, 279, 10.1089/rej.2006.9.279 · DOI
- Starzl TE, 1970, J Surg Res, 10, 291, 10.1016/0022-4804(70)90047-8 · DOI
- Smith AU, 1957, Proc R Soc Lond B Biol Sci, 147, 533, 10.1098/rspb.1957.0077 · DOI
- Karow AM, 1974, Organ Preservation for Transplantation, 3
- Khirabadi B, 2000, Transplantation, 70, 51
- Fahy GM, 2004, Cryobiology, 48, 157, 10.1016/j.cryobiol.2004.02.002 · DOI · Paper page
- Wang X, 2002, Nature, 415, 385, 10.1038/415385a · DOI
- Karlsson JO, 2000, Principles of Tissue Engineering, 293, 10.1016/B978-012436630-5/50028-3 · DOI
- Arav A, 2005, Hum Reprod, 20, 3554, 10.1093/humrep/dei278 · DOI
- Kaiser J, 2002, Science, 295, 1015, 10.1126/science.295.5557.1015 · DOI
- 10.1038/73737 · DOI
- Bedaiwy MA, 2003, Fertil Steril, 79, 594, 10.1016/S0015-0282(02)04842-2 · DOI
- Martinez-Madrid B, 2004, Fertil Steril, 82, 1390, 10.1016/j.fertnstert.2004.06.036 · DOI
- Hamilton R, 1973, J Surg Res, 14, 313, 10.1016/0022-4804(73)90033-4 · DOI
- Fahy GM, 1980, Cryobiology, 17, 371, 10.1016/0011-2240(80)90044-9 · DOI
- Elami A, 2008, J Thorac Cardiovasc Surg, 135, 666, 10.1016/j.jtcvs.2007.08.056 · DOI
- Toledo-Pereyra LH, 1982, J Surg Res, 32, 75, 10.1016/0022-4804(82)90188-3 · DOI
- Pegg DE, 1978, Cryobiology, 15, 618, 10.1016/0011-2240(78)90086-X · DOI
- Smith AU, 1961, Biological Effects of Freezing and Supercooling, 247
- Kubota S, 1976, Low Temp Med, 2, 95
- Pegg DE, 1982, Organ Preservation, Basic and Applied Aspects, 389, 10.1007/978-94-011-6267-8_58 · DOI
- Karow AM, 1975, Cryobiology, 12, 130, 10.1016/S0011-2240(75)80005-8 · DOI
- Hunt CJ, 1984, Cryobiology, 21, 385, 10.1016/0011-2240(84)90077-4 · DOI
- Pollack GA, 1986, Cryobiology, 23, 500, 10.1016/0011-2240(86)90059-3 · DOI
- Halasz NA, 1967, Surgery, 61, 417
- Halasz NA, 1968, Organ Perfusion and Preservation, 731
- Guttman FM, 1977, Cryobiology, 14, 559, 10.1016/0011-2240(77)90166-3 · DOI
- Lehr H, 1971, Transplant Proc, 3, 1565
- Fahy GM, 1980, Cryo Letters, 1, 312
- Fahy GM, 1981, Cryobiology, 18, 617, 10.1016/0011-2240(81)90150-4 · DOI
- Fahy GM, 1982, Organ Preservation, Basic and Applied Aspects, 399, 10.1007/978-94-011-6267-8_60 · DOI
- 10.1016/0011-2240(84)90079-8 · DOI · Paper page
- Fahy GM, 1982, Cryobiology, 19, 668, 10.1016/0011-2240(82)90257-7 · DOI
- Wowk B, 2005, Cryobiology, 51, 362
- Wowk B, 2007, Cryobiology, 330, 10.1016/j.cryobiol.2007.10.024 · DOI
- Wowk B, 2009, Cryobiology, 59
- Fahy GM, 2007, Vitrification in Assisted Reproduction: A User's Manual and Troubleshooting Guide
- 10.1038/313573a0 · DOI
- 10.1016/j.cryobiol.2003.11.004 · DOI · Paper page
- Fahy GM, 1995, Biological ice nucleation and its applications, 315
- Fahy GM, 1988, Low Temperature Biotechnology: Emerging Applications and Engineering Contributions, 113
- Courbiere B, 2005, Fertil Steril, 84, 1065, 10.1016/j.fertnstert.2005.03.079 · DOI
- Sugimoto M, 2000, Theriogenology, 53, 1093, 10.1016/S0093-691X(00)00255-7 · DOI
- Salehnia M, 2002, Exp Anim, 5, 509, 10.1538/expanim.51.509 · DOI
- Migishima F, 2003, Biol Reprod, 68, 881, 10.1095/biolreprod.102.007948 · DOI
- Brockbank KG, 2004, J Heart Valve Dis, 13, 297
- Armitage WJ, 2002, Invest Ophthalmol Vis Sci, 43, 2160
- Taylor MJ, 2004, Life in the frozen state, 603, 10.1201/9780203647073.ch22 · DOI
- Fahy GM, 1997, Cryobiology, 35, 114, 10.1006/cryo.1997.2026 · DOI
- Fahy GM, 1995, Cell Biology of Trauma
- Khirabadi BS, 1994, Cryobiology, 31, 597, 10.1006/cryo.1994.1002 · DOI
- Wu J, 2003, Microsurgery, 23, 1, 10.1002/micr.10090 · DOI
- Ofstad J, 1985, The kidney, physiology and pathophysiology, 471
- Kaissling B, 1979, Adv Anat Embryol Cell Biol, 56, 1
- 10.1006/cryo.2000.2243 · DOI
- Fahy GM, 1994, Biomed Instrum Technol, 28, 87
Cited by
Sorted by citations
Han, Z., Rao, J. S., Gangwar, L., Namsrai, B.-E., Pasek-Allen, J. L., Etheridge, M. L., Wolf, S. M., Pruett, T. L., Bischof, J. C., & Finger, E. B. (2023) · Nature Communications · doi:10.1038/s41467-023-38824-8 · Cited by 173
Messner, F., Guo, Y., Etra, J. W., & Brandacher, G. (2019) · Transplant International · doi:10.1111/tri.13432 · Cited by 29
Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation
Gangwar, L., Han, Z., Scheithauer, C., Namsrai, B.-E., Kantesaria, S., Goldstein, R., Etheridge, M. L., Finger, E. B., & Bischof, J. C. (2025) · Nature Communications · doi:10.1038/s41467-025-63483-2 · Cited by 26
Metadata
- Authors
- Fahy, G. M., Wowk, B., Pagotan, R., Chang, A., Phan, J., Thomson, B., & Phan, L.
- Venue
- Organogenesis
- Publisher
- Informa UK Limited
- Published
- 2009-07
- Volume
- 5
- Issue
- 3
- Pages
- 167-175
- ISSN
- 1547-6278
- 1555-8592
- Cited by
- 168
- References
- 0
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
