CoChat
Sign Up
Cited by 199

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma

Turchinovich, Andrey, Burwinkel, Barbara·2012·RNA Biology
DOI 10.4161/rna.21083PMC PMC3551861
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.4161/rna.21083

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1. Association of miRNAs with endogenous AGO1 and AGO2 proteins in MCF7 cells. (A) Correlation plot showing Ct values of miRNA signals detected in anti-AGO1 and anti-AGO2 pellets using TaqMan Low Density miRNA Arrays (TLDA). Note, the high correlation coefficient indicate low bias in binding toward AGO1 and AGO2 proteins for most miRNAs. (B) Ct values of the miRNA species on TLDA which were selected for verification with individual qPCR assays. (C) Individual TaqMan miRNA Assays of miR-16, miR-21, miR-24, miR-27a, miR-331 and miR-222 in anti-AGO1, anti-AGO2 and anti-α-tubulin (control) immunoprecipitates from MCF7 cells. Spiked in cel-miR-39 was used as control for isolation efficiency in all samples. Data are presented as Ct values normalized on both cel-miR-39 and the mean Ct of [miR-16, miR-21, miR-24, miR-27a, miR-331 and miR-222] within each sample (quantile normalization). Each bar represents mean + SD of three independent experiments. (D) Estimation of average miRNA association with each AGO protein. The means of Ct values of miRNAs in either anti-AGO1 or anti-AGO2 samples detected on the array (left) were compared with the relative content of AGO1 and AGO2 proteins detected by western blot (right) in the same immunopellets. To account for the difference in anti-AGO1 and anti-AGO2 antibody efficiency on western blot, equal amounts of recombinant FLAG-AGO1 (pAGO1) and FLAG-AGO2 (pAGO2) proteins were applied on the SDS gel. Note, the content of AGO1 protein in the anti-AGO1 coIP pellet was lower as compared with AGO2 protein in anti-AGO2 coIP pellet (~2,5 times as analyzed by densitometry). Accordingly, the observed difference in the mean Ct values between AGO1 and AGO2 coIP pellets corresponded to approximately 2,5 times difference in the total miRNA content.
Figure 1. Association of miRNAs with endogenous AGO1 and AGO2 proteins in MCF7 cells. (A) Correlation plot showing Ct values of miRNA signals detected in anti-AGO1 and anti-AGO2 pellets using TaqMan Low Density miRNA Arrays (TLDA). Note, the high correlation coefficient indicate low bias in binding toward AGO1 and AGO2 proteins for most miRNAs. (B) Ct values of the miRNA species on TLDA which were selected for verification with individual qPCR assays. (C) Individual TaqMan miRNA Assays of miR-16, miR-21, miR-24, miR-27a, miR-331 and miR-222 in anti-AGO1, anti-AGO2 and anti-α-tubulin (control) immunoprecipitates from MCF7 cells. Spiked in cel-miR-39 was used as control for isolation efficiency in all samples. Data are presented as Ct values normalized on both cel-miR-39 and the mean Ct of [miR-16, miR-21, miR-24, miR-27a, miR-331 and miR-222] within each sample (quantile normalization). Each bar represents mean + SD of three independent experiments. (D) Estimation of average miRNA association with each AGO protein. The means of Ct values of miRNAs in either anti-AGO1 or anti-AGO2 samples detected on the array (left) were compared with the relative content of AGO1 and AGO2 proteins detected by western blot (right) in the same immunopellets. To account for the difference in anti-AGO1 and anti-AGO2 antibody efficiency on western blot, equal amounts of recombinant FLAG-AGO1 (pAGO1) and FLAG-AGO2 (pAGO2) proteins were applied on the SDS gel. Note, the content of AGO1 protein in the anti-AGO1 coIP pellet was lower as compared with AGO2 protein in anti-AGO2 coIP pellet (~2,5 times as analyzed by densitometry). Accordingly, the observed difference in the mean Ct values between AGO1 and AGO2 coIP pellets corresponded to approximately 2,5 times difference in the total miRNA content.CC BY-NC · PubMed Central
Figure 2. TaqMan miRNA Low Density Arrays performed on total RNA extracted from anti-AGO1 and anti-AGO2 immunoprecipitates of blood plasma (A) and whole blood pellet (B). Blood plasma (80 μL) was combined with 20 μg the antibody. Blood pellet lysate (5 μL) combined with 3 μg of the antibody. MiRNAs which were undetected on the array (Ct > 32) were assigned Ct values of 40. Note, (1) some miRNAs were presented exclusively in the coIP pellets of particular AGO protein; (2) unlike in blood plasma, in blood pellet overwhelming majority of miRNAs were AGO2 associated.
Figure 2. TaqMan miRNA Low Density Arrays performed on total RNA extracted from anti-AGO1 and anti-AGO2 immunoprecipitates of blood plasma (A) and whole blood pellet (B). Blood plasma (80 μL) was combined with 20 μg the antibody. Blood pellet lysate (5 μL) combined with 3 μg of the antibody. MiRNAs which were undetected on the array (Ct > 32) were assigned Ct values of 40. Note, (1) some miRNAs were presented exclusively in the coIP pellets of particular AGO protein; (2) unlike in blood plasma, in blood pellet overwhelming majority of miRNAs were AGO2 associated.CC BY-NC · PubMed Central
Figure 3. (A) miRNA species in the blood plasma which showed significant bias toward either AGO1 (miR-21, miR-223) or AGO2 (miR-16, miR-451) proteins on TaqMan Low Density miRNA Array (above) and their verification with individual miRNA qPCR assays (below) in three independent experiments; (B) The same miRNA species demonstrated drastically different AGO1/AGO2 distribution in whole blood pellet: TLDA (above), individual qPCR assays (below). Data presented as Ct values normalized on cel-miR-39 and miR-16. Each bar represents mean + SD of three independent experiments (samples from different individuals). (C) The ΔCt (AGO1-AGO2) graph of miRNAs detected in both blood plasma and blood pellet. Note, for most of the miRNA there is no correlation of ΔCt (AGO1-AGO2) between plasma and pellet; (D) western blot analysis of AGO1 and AGO2 proteins in anti-AGO1 and anti-AGO2 coIP samples respectively. Note, unlike AGO2 protein, the amount of AGO1 in the immuprecipitate was below the detection limit.
Figure 3. (A) miRNA species in the blood plasma which showed significant bias toward either AGO1 (miR-21, miR-223) or AGO2 (miR-16, miR-451) proteins on TaqMan Low Density miRNA Array (above) and their verification with individual miRNA qPCR assays (below) in three independent experiments; (B) The same miRNA species demonstrated drastically different AGO1/AGO2 distribution in whole blood pellet: TLDA (above), individual qPCR assays (below). Data presented as Ct values normalized on cel-miR-39 and miR-16. Each bar represents mean + SD of three independent experiments (samples from different individuals). (C) The ΔCt (AGO1-AGO2) graph of miRNAs detected in both blood plasma and blood pellet. Note, for most of the miRNA there is no correlation of ΔCt (AGO1-AGO2) between plasma and pellet; (D) western blot analysis of AGO1 and AGO2 proteins in anti-AGO1 and anti-AGO2 coIP samples respectively. Note, unlike AGO2 protein, the amount of AGO1 in the immuprecipitate was below the detection limit.CC BY-NC · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 3 references

Research works printed but not linked: 36

  1. 10.1016/S0092-8674(04)00045-5 · DOI
  2. 10.1038/nature01957 · DOI
  3. 10.1101/gad.1262504 · DOI
  4. 10.1126/science.1062961 · DOI · Paper page
  5. 10.1016/j.cell.2006.03.043 · DOI
  6. 10.1016/j.pbi.2008.06.008 · DOI
  7. 10.1038/nrm2321 · DOI
  8. 10.1038/nrg2916 · DOI · Paper page
  9. 10.1186/gb-2008-9-2-210 · DOI
  10. 10.1016/j.molcel.2004.07.007 · DOI · Paper page
  11. 10.1126/science.1102513 · DOI · Paper page
  12. 10.1073/pnas.0800334105 · DOI
  13. 10.4161/rna.8.1.14300 · DOI
  14. 10.1073/pnas.0804549105 · DOI · Paper page
  15. 10.1002/ijc.25007 · DOI
  16. 10.1111/j.1471-4159.2011.07307.x · DOI
  17. 10.1373/clinchem.2010.147850 · DOI · Paper page
  18. 10.1002/ijc.25376 · DOI
  19. 10.1073/pnas.1019055108 · DOI · Paper page
  20. 10.1093/nar/gkr254 · DOI · Paper page
  21. 10.1007/978-1-61779-083-6_12 · DOI
  22. 10.1373/clinchem.2010.157198 · DOI
  23. 10.1371/journal.pone.0020769 · DOI
  24. 10.1371/journal.pone.0024145 · DOI · Paper page
  25. 10.1371/journal.pone.0002360 · DOI · Paper page
  26. 10.4161/rna.1.2.1066 · DOI
  27. 10.1016/j.cell.2007.05.056 · DOI
  28. 10.1016/j.cell.2007.05.057 · DOI
  29. 10.1093/nar/gkr1274 · DOI
  30. 10.1016/j.molcel.2009.09.028 · DOI
  31. 10.1016/j.molcel.2009.09.027 · DOI · Paper page
  32. 10.1038/nsmb1308 · DOI
  33. 10.1261/rna.901908 · DOI
  34. 10.1016/S0888-7543(03)00129-0 · DOI
  35. 10.1016/j.bbagrm.2008.01.007 · DOI
  36. 10.1016/j.bbagrm.2012.03.007 · DOI

Cited by

Sorted by citations

See all 199

Metadata

Authors
Turchinovich, A., & Burwinkel, B.
Venue
RNA Biology
Publisher
Informa UK Limited
Published
2012-08
Volume
9
Issue
8
Pages
1066-1075
ISSN
  • 1547-6286
  • 1555-8584
DOI
10.4161/rna.21083
PMC
PMC3551861
Cited by
199
References
3
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma. https://app.cochat.ai/papers/10.4161/rna.21083
The paper
Turchinovich, A., & Burwinkel, B. (2012). Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma. RNA Biology. https://doi.org/10.4161/rna.21083