Naturally Occurring Lactic Acid Bacteria Isolated from Tomato Pomace Silage
Open accessAbstract
Silage making has become a significant method of forage conservation worldwide. To determine how tomato pomace (TP) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (TPS) were evaluated at the 30th, 60th, and 90th days, respectively. In addition, 103 lactic acid bacteria were isolated from TPS. Based on the phenotypic and chemotaxonomic characteristics, 16S rDNA sequence and carbohydrate fermentation tests, the isolates were identified as 17 species namely: Lactobacillus coryniformis subsp. torquens (0.97%), Lactobacillus pontis (0.97%), Lactobacillus hilgardii (0.97%), Lactobacillus pantheris (0.97%), Lactobacillus amylovorus (1.9%), Lactobacillus panis (1.9%), Lactobacillus vaginalis (1.9%), Lactobacillus rapi (1.9%), Lactobacillus buchneri (2.9%), Lactobacillus parafarraginis (2.9%), Lactobacillus helveticus (3.9%), Lactobacillus camelliae (3.9%), Lactobacillus fermentum (5.8%), Lactobacillus manihotivorans (6.8%), Lactobacillus plantarum (10.7%), Lactobacillus harbinensis (16.5%) and Lactobacillus paracasei subsp. paracasei (35.0%). This study has shown that TP can be well preserved for 90 days by ensilaging and that TPS is not only rich in essential nutrients, but that physiological and biochemical properties of the isolates could provide a platform for future design of lactic acid bacteria (LAB) inoculants aimed at improving the fermentation quality of silage.
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A study of LAB diversity in tomato pomace silage reported that 4% of the isolates belonged to Lb. helveticus.
“a study of lactic acid bacteria diversity in tomato pomace silage reported that 4% of the isolates belonged to Lb. helveticus [41]”
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- Authors
- Wu, J.-J., Du, R.-P., Gao, M., Sui, Y.-Q., Xiu, L., & Wang, X.
- Affiliations
- Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences (CN)
- Corresponding author
- Wang, Xiao
- Venue
- Asian-Australasian Journal of Animal Sciences
- Published
- 2014-04-22
- Volume
- 27
- Issue
- 5
- Pages
- 648-657
- ISSN
- 1011-2367 (ISSN-L)
- 1976-5517
- PMC
- PMC4093187
- OpenAlex
- W2138940248
- Cited by
- 31
- References
- 39
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 3 · 2017: 3 · 2016: 5 · 2015: 3
- FWCI
- 5.26
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Asian-Australasian Journal of Animal Sciences (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- tomato pomace, silage, lactic acid bacteria, Lactobacillus, silage fermentation, forage preservation, silage microbiology, silage inoculants, Lacticaseibacillus paracasei subsp. paracasei, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus buchneri, Lactobacillus rhamnosus
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