Distinct cellular and molecular mechanisms for β3 adrenergic receptor-induced beige adipocyte formation
Open accessAbstract
Beige/brite adipocytes are induced within white adipose tissues (WAT) and, when activated, consume glucose and fatty acids to produce heat. Classically, two stimuli have been used to trigger a beiging response: cold temperatures and β3-adrenergic receptor (Adrb3) agonists. These two beiging triggers have been used interchangeably but whether these two stimuli may induce beiging differently at cellular and molecular levels remains unclear. Here, we found that cold-induced beige adipocyte formation requires Adrb1, not Adrb3, activation. Adrb1 activation stimulates WAT resident perivascular (Acta2+) cells to form cold-induced beige adipocytes. In contrast, Adrb3 activation stimulates mature white adipocytes to convert into beige adipocytes. Necessity tests, using mature adipocyte-specific Prdm16 deletion strategies, demonstrated that adipocytes are required and are predominant source to generate Adrb3-induced, but not cold-induced, beige adipocytes. Collectively, we identify that cold temperatures and Adrb3 agonists activate distinct cellular populations that express different β-adrenergic receptors to induce beige adipogenesis.
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ADRB3 is the key mediator for the stimulatory effects of catecholamines on WAT lipolysis and BAT thermogenesis.
“ADRB3 is the key mediator for the stimulatory effects of catecholamines on WAT lipolysis and BAT thermogenesis (Claustre et al, 2008; Jiang et al, 2017).”
From Exercise‐induced α‐ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1‐dependent adrenal activation (2020) · cites this paper as Jiang et al, 2017
The Introduction (page unavailable) says norepinephrine signals through Adrb3 to stimulate thermogenic action in mature brown adipocytes. It does not identify Adrb3 as the key mediator of catecholamine-stimulated WAT lipolysis; the Results (page unavailable) report reduced triglycerides after treatment with an Adrb3 agonist, but do not establish that broader claim. (LLM, conf=0.85)
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Obesity: A neuroimmunometabolic perspective
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“ADRB3 is the key mediator for the stimulatory effects of catecholamines on WAT lipolysis and BAT thermogenesis (Claustre et al , 2008; Jiang et al , 2017).”
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- Authors
- Jiang, Y., Berry, D. C., & Graff, J. M.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Cornell University (US)
- Corresponding author
- Berry, Daniel C
- Graff, Jonathan M
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2017-10-11
- Volume
- 6
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC5667933
- OpenAlex
- W2763289003
- Cited by
- 135
- References
- 58
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 15 · 2023: 17 · 2022: 19 · 2021: 21 · 2020: 22 · 2019: 14 · 2018: 6 · 2017: 2
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (K01 DK109027, K01 DK111771, R01 DK088220, R01 DK064261, R01 DK066556)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- beige adipocytes, beta3-adrenergic receptor, ADRB1, white adipose tissue, perivascular cells, PRDM16, adipogenesis, thermogenesis
- MeSH terms
- Adipocytes, Beige, Animals, Cell Differentiation, Cold Temperature, Mice, Inbred C57BL, Receptors, Adrenergic, beta-1, Receptors, Adrenergic, beta-3
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