State-dependent cell-type-specific membrane potential dynamics and unitary synaptic inputs in awake mice
Open accessAbstract
The cellular and synaptic mechanisms driving cell-type-specific function during various cortical network activities and behaviors are poorly understood. Here, we targeted whole-cell recordings to two classes of inhibitory GABAergic neurons in layer 2/3 of the barrel cortex of awake head-restrained mice and correlated spontaneous membrane potential dynamics with cortical state and whisking behavior. Using optogenetic stimulation of single layer 2/3 excitatory neurons we measured unitary excitatory postsynaptic potentials (uEPSPs) across states. During active states, characterized by whisking and reduced low-frequency activity in the local field potential, parvalbumin-expressing neurons depolarized and, albeit in a small number of recordings, received uEPSPs with increased amplitude. In contrast, somatostatin-expressing neurons hyperpolarized and reduced firing rates during active states without consistent change in uEPSP amplitude. These results further our understanding of neocortical inhibitory neuron function in awake mice and are consistent with the hypothesis that distinct genetically-defined cell classes have different state-dependent patterns of activity.
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The degree to which the synaptic input underlying Vm fluctuations is temporally correlated can vary with overall drive to a network or with firing rates and is regulated by behavioural state transitions during wakefulness.
“The degree to which the synaptic input underlying Vm fluctuations is temporally correlated can vary with overall drive to a network or with firing rates70 and is regulated by behavioural state transitions during wakefulness29,71–74.”
From Mechanisms underlying gain modulation in the cortex (2020) · cites this paper as [72]
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- Authors
- Pala, A., & Petersen, C. C.
- Affiliations
- Georgia Institute of Technology (US)
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Pala, Aurélie
- Petersen, Carl CH
- Venue
- eLife
- Publisher
- eLife Sciences Publications, Ltd
- Published
- 2018-07-27
- Volume
- 7
- ISSN
- 2050-084X (ISSN-L)
- PMC
- PMC6063730
- OpenAlex
- W2883061260
- Cited by
- 31
- References
- 41
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 8 · 2019: 9 · 2018: 1
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- eLife (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- Swiss National Science Foundation (310030B_166595)
- European Research Council (ERC-ADG-293660)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- cortical state, membrane potential dynamics, inhibitory interneurons, parvalbumin neurons, somatostatin neurons, barrel cortex, layer 2/3, spontaneous activity, local field potentials, GABAergic neurons, optogenetic stimulation, awake mice
- MeSH terms
- Action Potentials, Animals, Female, Male, Membrane Potentials, Synaptic Transmission, Somatosensory Cortex, Vibrissae, Wakefulness, Mice, GABAergic Neurons, Optogenetics
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