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Cited by 60Neuroscience and Neuropharmacology ResearchAdvanced Electron Microscopy Techniques and ApplicationsMemory and Neural Mechanisms

Three-dimensional synaptic organization of the human hippocampal CA1 field

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Montero-Crespo, Marta, Dominguez-Alvaro, Marta, Rondon-Carrillo, Patricia, Alonso-Nanclares, Lidia, DeFelipe, Javier, Blazquez-Llorca, Lidia·2020·eLife
DOI 10.7554/elife.57013PMC PMC7375818

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Abstract

The hippocampal CA1 field integrates a wide variety of subcortical and cortical inputs, but its synaptic organization in humans is still unknown due to the difficulties involved studying the human brain via electron microscope techniques. However, we have shown that the 3D reconstruction method using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) can be applied to study in detail the synaptic organization of the human brain obtained from autopsies, yielding excellent results. Using this technology, 24,752 synapses were fully reconstructed in CA1, revealing that most of them were excitatory, targeting dendritic spines and displaying a macular shape, regardless of the layer examined. However, remarkable differences were observed between layers. These data constitute the first extensive description of the synaptic organization of the neuropil of the human CA1 region.

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  • The use of a lower concentration of potassium ferrocyanide (0.1%) shows an improvement in membrane visualization while still allowing the PSD of the SS to be identified.

    “In conclusion, the use of a lower concentration of potassium ferrocyanide (0.1%), as we propose here, shows an improvement in membrane visualization, while still allowing the PSD of the SS to be identified (Domínguez-Álvaro et al., 2018, 2019, 2021a,b; Montero-Crespo et al., 2020, 2021; Cano-Astorga et al., 2021, 2023).”

    From Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy (2024) · cites this paper as Montero-Crespo et al., 2020

    The Materials and methods (Tissue processing for EM; page unavailable) report using 0.1% potassium ferrocyanide. The 3D analysis of synapses section says symmetric synapses could be identified by their thin postsynaptic densities. The paper does not report a comparison showing that this concentration improved membrane visualization. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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References 109 · Cited by 60

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Authors
Montero-Crespo, M., Dominguez-Alvaro, M., Rondon-Carrillo, P., Alonso-Nanclares, L., DeFelipe, J., & Blazquez-Llorca, L.
Affiliations
  • Instituto Cajal, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
  • Laboratorio Cajal de Circuitos Corticales, Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Madrid, Spain
  • Consejo Superior de Investigaciones Científicas (ES)
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2020-07-21
Volume
9
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.57013
PMC
PMC7375818
OpenAlex
W3044074257
Cited by
60
References
109
Citations by year
2026: 3 · 2025: 7 · 2024: 18 · 2023: 14 · 2022: 7 · 2021: 7 · 2020: 4
FWCI
3.67
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CB06/05/0066)
  • Spanish Ministry of Science and Innovation (PGC2018-094307-B-I00, Cajal Blue Brain Project)
  • Horizon 2020 Framework Programme (785907)
  • Alzheimer's Association (ZEN-15-321663)
  • Universidad Nacional de Educación a Distancia (2014-040-UNED-POST)
  • Ministry of Education, Culture and Sports, Spain (FPU14/02245)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
hippocampal CA1, synaptic organization, human brain, dendritic spines, excitatory synapses, neuropil, postmortem brain
MeSH terms
Adult, Aged, Aged, 80 and over, Autopsy, Cadaver, Female, Humans, Male, Microscopy, Electron, Scanning, Middle Aged, Molecular Conformation, CA1 Region, Hippocampal

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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