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Cited by 299Conducting polymers and applicationsNeuroscience and Neural EngineeringPolymer Surface Interaction Studies

Effect of Immobilized Nerve Growth Factor on Conductive Polymers: Electrical Properties and Cellular Response

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Kim, D.‐H., Richardson‐Burns, S. M., Hendricks, J. L., Sequera, C., Martin, D. C.·2007·Advanced Functional Materials
DOI 10.1002/adfm.200500594
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Abstract

The use of biologically active dopants in conductive polymers allows the polymer to be tailored for specific applications. The incorporation of nerve growth factor (NGF) as a co‐dopant in the electrochemical deposition of conductive polymers is evaluated for its ability to elicit specific biological interactions with neurons. The electrochemical properties of the NGF‐modified conducting polymers are studied by impedance spectroscopy and cyclic voltammetry. Impedance measurements at the neurobiologically important frequency of 1 kHz reveal that the minimum impedance of the NGF‐modified polypyrrole (PPy) film, 15 kΩ, is lower than the minimum impedance of peptide‐modified PPy film (360 kΩ). Similar results are found with NGF‐modified poly(3,4‐ethylene dioxythiophene) (PEDOT). The microstructure of the conductive polymer films is characterized by optical microscopy and electron microscopy and indicates that the NGF‐functionalized polymer surface topology is similar to that of the unmodified polymer film. Optical and fluorescence microscopy reveal that PC‐12 (rat pheochromacytoma) cells adhered to the NGF‐modified substrate and extended neurites on both PPy and PEDOT, indicating that the NGF in the polymer film is biologically active. Taken together these data indicate that the incorporation of NGF can modify the biological interactions of the electrode without compromising the conductive properties or the morphology of the polymeric film.

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  • NGF incorporated as a codopant in the electrochemical polymerization of PEDOT and PPy resulted in increased neurite outgrowth of PC-12 cells on the NGF-containing polymer substrates.

    “NGF has been incorporated as a codopant in the electrochemical polymerization of PEDOT and PPy, where PC-12 cells responded with increased neurite outgrowth on the NGFcontaining polymer substrates.53”

    From Regenerative Electrode Interfaces for Neural Prostheses. (2016) · cites this paper as [53]

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Authors
Kim, D. ‐., Richardson‐Burns, S. M., Hendricks, J. L., Sequera, C., & Martin, D. C.
Affiliations
  • Duke University (US)
  • University of Michigan (US)
Corresponding author
Martin, D. C.
ORCID
Venue
Advanced Functional Materials
Publisher
Wiley
Published
2006-11-20
Volume
17
Issue
1
Pages
79-86
ISSN
  • 1616-301X (ISSN-L)
  • 1616-3028
DOI
10.1002/adfm.200500594
OpenAlex
W1977164754
Cited by
299
References
44
Citations by year
2026: 9 · 2025: 7 · 2024: 10 · 2023: 12 · 2022: 14 · 2021: 21 · 2020: 16 · 2019: 17 · 2018: 23 · 2017: 21 · 2016: 27 · 2015: 25 · 2014: 14 · 2013: 13 · 2012: 14
FWCI
8.62
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Advanced Functional Materials (publishedVersion)
  • Deep Blue (University of Michigan) (submittedVersion)
Funders
  • National Science Foundation (0518079, 0084304)
  • National Aeronautics and Space Administration
  • University of Michigan
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke
  • Division of Materials Research
Topics
  • Physical Sciences › Materials Science › Polymers and Plastics › Conducting polymers and applications
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
Keywords
nerve growth factor, conducting polymers, polypyrrole, PEDOT, electrodeposition, co-doping, electrical impedance, PC12 cells, neurite outgrowth, cell adhesion

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