Composite living fibers for creating tissue constructs using textile techniques
Open accessAbstract
The fabrication of cell‐laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control over the color pattern and directional mechanical properties. The use of textile techniques in tissue engineering, however, demands the presence of cell‐laden fibers that can withstand the mechanical stresses during the assembly process. Here, the concept of composite living fibers (CLFs) in which a core of load bearing synthetic polymer is coated by a hydrogel layer containing cells or microparticles is introduced. The core thread is drawn sequentially through reservoirs containing a cell‐laden prepolymer and a crosslinking reagent. The thickness of the hydrogel layer increases linearly with to the drawing speed and the prepolymer viscosity. CLFs are fabricated and assembled using regular textile processes including weaving, knitting, braiding, winding, and embroidering, to form cell‐laden structures. Cellular viability and metabolic activity are preserved during CLF fabrication and assembly, demonstrating the feasibility of using these processes for engineering functional 3D tissue constructs.
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The observed cell density range is typical for hydrogel encapsulation.
“which is a typical cell density range for hydrogel encapsulation [7]”
From A liver-on-a-chip platform with bioprinted hepatic spheroids (2016) · cites this paper as [7]
The Experimental Section (Preparation of Cell Suspension in Sodium Alginate; page unavailable) reports using 2–5 × 10^6 cells mL−1 in an alginate suspension for cell-laden hydrogel fibers. This supports the range as an example of hydrogel encapsulation, but the paper does not establish that it is typical across hydrogel encapsulation generally. (LLM, conf=0.82)
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- Authors
- Akbari, M., Tamayol, A., Laforte, V., Annabi, N., Najafabadi, A. H., Khademhosseini, A., & Juncker, D.
- Affiliations
- Brigham and Women's Hospital (US)
- McGill University and Genome Quebec Innovation Centre McGill University Quebec Montreal H3A 0G1 Canada
- Harvard University (US)
- McGill University and Génome Québec Innovation Centre (CA)
- Corresponding author
- Khademhosseini, Ali
- Juncker, David
- ORCID
- Venue
- Advanced Functional Materials
- Publisher
- Wiley
- Published
- 2014-03-31
- Volume
- 24
- Issue
- 26
- Pages
- 4060-4067
- ISSN
- 1616-301X (ISSN-L)
- 1616-3028
- PMC
- PMC4233137
- OpenAlex
- W2062608800
- Cited by
- 167
- References
- 27
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 8 · 2023: 12 · 2022: 6 · 2021: 16 · 2020: 15 · 2019: 21 · 2018: 15 · 2017: 18 · 2016: 20 · 2015: 13 · 2014: 4
- FWCI
- 9.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Functional Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Science Foundation (DMR 0847287)
- National Institutes of Health (HL092836, DE019024, EB012597, AR057837, DE021468, HL099073, EB008392)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- tissue engineering, textile industry, hydrogel coating, mechanical anisotropy, braiding, cell viability, 3D tissue constructs
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