Cited by 8633D Printing in Biomedical ResearchMicrofluidic and Capillary Electrophoresis ApplicationsCellular Mechanics and Interactions

Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low‐Viscosity Bioink

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Colosi, Cristina, Shin, Su Ryon, Manoharan, Vijayan, Massa, Solange, Costantini, Marco, Barbetta, Andrea, Dokmeci, Mehmet Remzi, Dentini, Mariella +1 more·2016·Advanced Materials

Abstract

A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The technique incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures. The extrusion system is then coupled to a microfluidic device to control the bioink arrangement deposition, demonstrating the versatility of the bioprinting technique. This low-viscosity cell-responsive bioink promotes cell migration and alignment within each fiber organizing the encapsulated cells.

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Crossref · 10.1002/adma.201503310

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  • It has been a major challenge to realize ECM-like heterogeneous composition in microfluidic channels of tumor-on-a-chip.

    “It has been a major challenge to realize ECM-like heterogeneous composition in microfluidic channels of tumor-on-a-chip152.”

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  1. [18]Gregor ERM Grant T Rees David A Smith Peter JC Thom David FEBS letters 1973 32 Akbari M Tamayol A Laforte V Annabi N Najafabadi AH Khademhosseini A Juncker D Adv Funct Mater 2014 24 4060 25411576 PMC4233137
  2. [25]Colosi C Costantini M Latini R Ciccarelli S Stampella A Barbetta A Massimi M Devirgiliis LC Dentini M J. Mater. Chem. B 2014 2 13 32261874
  3. [28]Kolesky DB Truby RL Gladman A Busbee TA Homan KA Lewis JA Advanced Materials 2014 26 3124 24550124
  4. [36]Patel PN Gobin AS West JL Patrick CW Jr Tissue Eng 2005 11 1498 16259604 Engler AJ Sen S Sweeney HL Discher DE Cell 2006 126 677 16923388 Hutson CB Nichol JW Aubin H Bae H Yamanlar S Al-Haque S Koshy ST Khademhosseini A Tissue Eng Part A 2011 17 13–14 1713 21306293 PMC3118706

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Authors
Colosi, C., Shin, S. R., Manoharan, V., Massa, S., Costantini, M., Barbetta, A., Dokmeci, M. R., Dentini, M., & Khademhosseini, A.
Affiliations
  • Brigham and Women's Hospital (US)
  • Sapienza University of Rome (IT)
Corresponding author
  • Dentini, Mariella
  • Khademhosseini, Ali
Venue
Advanced Materials
Publisher
Wiley
Published
2015-11-26
Volume
28
Issue
4
Pages
677-684
ISSN
  • 0935-9648 (ISSN-L)
  • 1521-4095
OpenAlex
W2174690427
Cited by
863
References
54
Citations by year
2026: 31 · 2025: 73 · 2024: 69 · 2023: 75 · 2022: 99 · 2021: 109 · 2020: 102 · 2019: 106 · 2018: 90 · 2017: 79 · 2016: 29
FWCI
32.79
Type
journal article
Language
en
Open access
Locations
  • Advanced Materials (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Funders
  • National Institutes of Health (HL092836, EB02597, AR057837, HL099073)
  • National Science Foundation (DMR0847287)
  • National Sciences and Engineering Research Council of Canada
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
bioprinting, coaxial extrusion, 3D tissue constructs, cell migration, cell alignment, cell-laden bioinks
MeSH terms
Alginates, Cell Survival, Extracellular Matrix, Gelatin, Hexuronic Acids, Humans, Ultraviolet Rays, Viscosity, Microscopy, Confocal, Hydrogel, Polyethylene Glycol Dimethacrylate, Glucuronic Acid, Tissue Engineering, Microfluidic Analytical Techniques, Tissue Scaffolds, Human Umbilical Vein Endothelial Cells, Bioprinting, Printing, Three-Dimensional

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