Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low‐Viscosity Bioink
Open accessAbstract
A novel bioink and a dispensing technique for 3D tissue-engineering applications are presented. The technique incorporates a coaxial extrusion needle using a low-viscosity cell-laden bioink to produce highly defined 3D biostructures. The extrusion system is then coupled to a microfluidic device to control the bioink arrangement deposition, demonstrating the versatility of the bioprinting technique. This low-viscosity cell-responsive bioink promotes cell migration and alignment within each fiber organizing the encapsulated cells.
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It has been a major challenge to realize ECM-like heterogeneous composition in microfluidic channels of tumor-on-a-chip.
“It has been a major challenge to realize ECM-like heterogeneous composition in microfluidic channels of tumor-on-a-chip152.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [152]
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- Authors
- Colosi, C., Shin, S. R., Manoharan, V., Massa, S., Costantini, M., Barbetta, A., Dokmeci, M. R., Dentini, M., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- Sapienza University of Rome (IT)
- Corresponding author
- Dentini, Mariella
- Khademhosseini, Ali
- ORCID
- Venue
- Advanced Materials
- Publisher
- Wiley
- Published
- 2015-11-26
- Volume
- 28
- Issue
- 4
- Pages
- 677-684
- ISSN
- 0935-9648 (ISSN-L)
- 1521-4095
- PMC
- PMC4804470
- OpenAlex
- W2174690427
- Cited by
- 863
- References
- 54
- Citations by year
- 2026: 31 · 2025: 73 · 2024: 69 · 2023: 75 · 2022: 99 · 2021: 109 · 2020: 102 · 2019: 106 · 2018: 90 · 2017: 79 · 2016: 29
- FWCI
- 32.79
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- Funders
- National Institutes of Health (HL092836, EB02597, AR057837, HL099073)
- National Science Foundation (DMR0847287)
- National Sciences and Engineering Research Council of Canada
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- bioprinting, coaxial extrusion, 3D tissue constructs, cell migration, cell alignment, cell-laden bioinks
- MeSH terms
- Alginates, Cell Survival, Extracellular Matrix, Gelatin, Hexuronic Acids, Humans, Ultraviolet Rays, Viscosity, Microscopy, Confocal, Hydrogel, Polyethylene Glycol Dimethacrylate, Glucuronic Acid, Tissue Engineering, Microfluidic Analytical Techniques, Tissue Scaffolds, Human Umbilical Vein Endothelial Cells, Bioprinting, Printing, Three-Dimensional
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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