Cited by 1273D Printing in Biomedical ResearchCancer Cells and MetastasisMicrofluidic and Capillary Electrophoresis Applications

Biomimetic Model of Tumor Microenvironment on Microfluidic Platform

Chung, Minhwan, Ahn, Jungho, Son, Kyungmin, Kim, Sudong, Jeon, Noo Li·2017·Advanced Healthcare Materials

Abstract

The “Tumor microenvironment” (TME) is a complex, interacting system of the tumor and its surrounding environment. The TME has drawn more attention recently in attempts to overcome current drug resistance and the recurrence of cancer by understanding the cancer and its microenvironment systematically, beyond past reductionist approaches. However, a lack of experimental tools to dissect the intricate interactions has hampered in‐depth research into the TME. Here, a biomimetic TME model using a microfluidic platform is presented, which enables the interaction between TME constituents to be studied in a comprehensive manner. Paracrine interactions of cocultured tumor cell lines (SK‐OV‐3, MKN‐74, and SW620) with primary fibroblasts show marked morphological changes in the tumor cells, depending on the type of tumor cells, and, importantly, the composition of the extracellular matrix. Furthermore, this model allows direct observation of angiogenesis induced by the tumor–stroma interaction. Finally, reconstituting simultaneous angiogenesis and lymphangiogenesis induced by the tumor‐stromal interaction with TME mimicking extrinsic factors is enabled. It is believed that the in vitro biomimetic model and the experimental concepts described will help to shed light on the complex biology of the TME.

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Crossref · 10.1002/adhm.201700196

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  • Conventional 2D cell monolayers and 3D cell culture models are unable to recapitulate the complex biochemical and biophysical factors of the tumor microenvironment in vivo because they lack the systemic nature of tumors, leading to weak predictive ability.

    “2D cell monolayers and three-dimensional cell culture models have been widely employed as initial screening models to provide a cost-efficient and simplified method to elucidate the mechanism of cancer biology or identify the efficacy and safety of a drug candidate14–16; however, they are unable to recapitulate complex biochemical and biophysical factors of the tumor microenvironment (TME) in vivo”

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Authors
Chung, M., Ahn, J., Son, K., Kim, S., & Jeon, N. L.
Affiliations
Seoul National University (KR)
Corresponding author
Jeon, Noo Li
Venue
Advanced Healthcare Materials
Publisher
Wiley
Published
2017-05-24
Volume
6
Issue
15
ISSN
  • 2192-2640 (ISSN-L)
  • 2192-2659
OpenAlex
W2620210433
Cited by
126
References
46
Citations by year
2026: 3 · 2025: 6 · 2024: 14 · 2023: 10 · 2022: 14 · 2021: 23 · 2020: 23 · 2019: 18 · 2018: 14 · 2017: 1
FWCI
6.89
Type
journal article
Language
en
Open access
closed
Locations
  • Advanced Healthcare Materials (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Science, ICT and Future Planning (NRF‐2015R1A2A1A09005662, 2016R1A4A1010796)
  • Seoul National University
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
tumor microenvironment, biomimetic systems, microfluidic platform, tumor-stroma interactions, extracellular matrix, paracrine signaling, co-culture, fibroblasts, cancer cell lines, angiogenesis, lymphangiogenesis, chemoresistance
MeSH terms
Equipment Design, Humans, Materials Testing, Neoplasms, Experimental, Equipment Failure Analysis, Tissue Engineering, Biomimetic Materials, Cell Line, Tumor, Lab-On-A-Chip Devices, Tumor Microenvironment, Batch Cell Culture Techniques

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