Next-Generation Liver Medicine Using Organoid Models
Open accessAbstract
“Liver medicine” refers to all diagnostic and treatment strategies of diseases and conditions that cause liver failure directly or indirectly. Despite significant advances in the field of liver medicine in recent years, improved tools are needed to efficiently define pathophysiology of liver diseases and provide effective therapeutic options to patients. Recently, organoid technology has been established as the state-of-the-art cell culture tool to study human biology in health and disease. In general, organoids are simplified three dimensional (3D) mini-organ structures that can be grown in a 3D matrix where the structural and functional aspects of real organs are efficiently recapitulated. The generation of organoids is facilitated by exogenous factors that regulate multiple signaling pathways and promote the self-renewal, proliferation, and differentiation of the cells to promote spontaneous self-organization and tissue-specific organogenesis. Newly established protocols suggest that liver-specific organoids can be derived from either pluripotent stem cells or liver specific stem/progenitor cells. Today, robust and long-term cultures of organoids with the closest physiology to in vivo liver, in terms of cellular composition and function, open a new era in studying and understanding the disease pathology as well as high-throughput drug screening. Of note, these next-generation cell culture systems carry an immense potential to be further improved by genome editing and bioengineering technologies to foster the development of patient-specific therapeutic options for clinical applications. Here, we will discuss recent advances and challenges on the generation of human liver organoids and highlight emerging concepts on their potential applications in liver medicine.
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Three-dimensional cell cultures, especially organoids, have been proposed for mimicking the architecture and genetic expression of native human liver tissue.
“To overcome the limitations of these 2D in vitro models, three-dimensional (3D) cell cultures-especially organoids-have been proposed for mimicking the architecture and genetic expression of native human liver tissue (Akbari et al., 2019;Sorrentino et al., 2020).”
From Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip (2024) · cites this paper as Akbari et al., 2019
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Social Responsibilities of Vietnam’s Exporter Enterprises Toward Consumer Loyalty in the EU Market
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Organoids: New Research Tool in Cancer Diagnostics and Therapeutics
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- Authors
- Akbari, S., Arslan, N., Senturk, S., & Erdal, E.
- Affiliations
- Izmir Biomedicine and Genome Center (TR)
- Dokuz Eylül University (TR)
- Corresponding author
- Erdal, Esra
- Venue
- Frontiers in Cell and Developmental Biology
- Publisher
- Frontiers Media SA
- Published
- 2019-12-20
- Volume
- 7
- Pages
- 345-345
- ISSN
- 2296-634X (ISSN-L)
- PMC
- PMC6933000
- OpenAlex
- W2994771578
- Cited by
- 72
- References
- 137
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 10 · 2023: 15 · 2022: 6 · 2021: 19 · 2020: 9
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Cell and Developmental Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (115S465)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- liver organoids, liver disease, liver failure, pluripotent stem cells, 3D cell culture, organogenesis, high-throughput drug screening, genome editing, bioengineering, patient-specific therapy
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