Biocatalytic reduction of short-chain carboxylic acids into their corresponding alcohols with syngas fermentation
Abstract
Short-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed with catalytic hydrogenation or with strong chemical reducing agents, here, a biological conversion route was explored. The potential of carboxydotrophic bacteria, such as Clostridium ljungdahlii and Clostridium ragsdalei, as biocatalysts for conversion of short-chain carboxylic acids into alcohols, using syngas as a source of electrons and energy is demonstrated. Acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, and n-caproic acid were converted into their corresponding alcohols. Furthermore, biomass yields and fermentation stoichiometry from the experimental data were modeled to determine how much metabolic energy C. ljungdahlii generated during syngas fermentation. An ATP yield of 0.4-0.5 mol of ATP per mol CO consumed was calculated in the presence of hydrogen. The ratio of protons pumped across the cell membrane versus electrons transferred from ferredoxin to NAD(+) via the Rnf complex is suggested to be 1.0. Based on these results, we provide suggestions how n-butyric acid to n-butanol conversion via syngas fermentation can be further improved.
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Pure cultures of C. ljungdahlii and "C. ragsdalei" have been reported to convert externally added carboxylic acids (acetate, propionate, n-butyrate, iso-butyrate, n-valerate, and n-caproate) into their corresponding alcohols using syngas as the source of electrons.
“Pure cultures of C. ljungdahlii and “C. ragsdalei” have been reported to convert externally added carboxylic acids (acetate, propionate, n-butyrate, iso-butyrate, n-valerate, and n-caproate) into their corresponding alcohols using syngas as the source of electrons (Perez et al., 2013; Isom et al., 2015).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Perez et al., 2013
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- Authors
- Perez, J. M., Richter, H., Loftus, S. E., & Angenent, L. T.
- Affiliations
- Cornell University (US)
- Corresponding author
- Angenent, Largus T.
- Venue
- Biotechnology and Bioengineering
- Publisher
- Wiley
- Published
- 2013-01-15
- Volume
- 110
- Issue
- 4
- Pages
- 1066-1077
- ISSN
- 0006-3592 (ISSN-L)
- 1097-0290
- OpenAlex
- W1975537901
- Cited by
- 126
- References
- 37
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 8 · 2023: 5 · 2022: 9 · 2021: 7 · 2020: 15 · 2019: 7 · 2018: 7 · 2017: 15 · 2016: 12 · 2015: 8 · 2014: 13 · 2013: 14
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Keywords
- short-chain carboxylic acids, carboxydotrophic bacteria, syngas fermentation, biocatalytic reduction, Clostridium ljungdahlii, acetic acid, propionic acid, valeric acid, hexanoic acid, isobutyric acid, ATP yield, Rnf complex, proton translocation
- MeSH terms
- Alcohols, Carboxylic Acids, Clostridium, Fermentation, Gases, Oxidation-Reduction, Biomass, Biocatalysis
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